RecQ helicase enhances homologous recombination in plants

AbstractRecQ helicase is a key component in the RecF pathway of Escherichia coli for initiation of homologous recombination. Here, we demonstrate that transient expression of RecQ gene in rice embryogenic cell increases the homologous recombination efficiency as much as 4-fold. Further experiments reveal that this effect is influenced by the RecQ dosage. Stable expression of RecQ in rice dramatically increases the homologous recombination events 20- to 40-fold in leaf tissue from different transgenic lines. This is the first evidence indicating that overexpression of RecQ gene can stimulate homologous recombination in plants

Temperature effects on the nuclear symmetry energy and symmetry free energy with an isospin and momentum dependent interaction

Within a self-consistent thermal model using an isospin and momentum dependent interaction (MDI) constrained by the isospin diffusion data in heavy-ion collisions, we investigate the temperature dependence of the symmetry energy $E_{sym}(\rho, T)$ and symmetry free energy $F_{sym}(\rho, T)$ for hot, isospin asymmetric nuclear matter. It is shown that the symmetry energy $E_{sym}(\rho, T)$ generally decreases with increasing temperature while the symmetry free energy $F_{sym}(\rho, T)$ exhibits opposite temperature dependence. The decrement of the symmetry energy with temperature is essentially due to the decrement of the potential energy part of the symmetry energy with temperature. The difference between the symmetry energy and symmetry free energy is found to be quite small around the saturation density of nuclear matter. While at very low densities, they differ significantly from each other. In comparison with the experimental data of temperature dependent symmetry energy extracted from the isotopic scaling analysis of intermediate mass fragments (IMF's) in heavy-ion collisions, the resulting density and temperature dependent symmetry energy $E_{sym}(\rho, T)$ is then used to estimate the average freeze-out density of the IMF's.used to estimate the average freeze-out density of the IMF's.Comment: 9 pages, 7 figures, 1 figure added to show the temperature dependence of the potential and kinetic parts of the symmetry energy. Revised version to appear in PR

Nuclear symmetry potential in the relativistic impulse approximation

Using the relativistic impulse approximation with the Love-Franey \textsl{NN} scattering amplitude developed by Murdock and Horowitz, we investigate the low-energy (100 MeV$\leq E_{\mathrm{kin}}\leq 400$ MeV) behavior of the nucleon Dirac optical potential, the Schr\"{o}dinger-equivalent potential, and the nuclear symmetry potential in isospin asymmetric nuclear matter. We find that the nuclear symmetry potential at fixed baryon density decreases with increasing nucleon energy. In particular, the nuclear symmetry potential at saturation density changes from positive to negative values at nucleon kinetic energy of about 200 MeV. Furthermore,the obtained energy and density dependence of the nuclear symmetry potential is consistent with those of the isospin- and momentum-dependent MDI interaction with $x=0$, which has been found to describe reasonably both the isospin diffusion data from heavy-ion collisions and the empirical neutron-skin thickness of $^{208}$Pb.Comment: 8 pages, 5 figures, revised version to appear in PR

Differential isospin-fractionation in dilute asymmetric nuclear matter

The differential isospin-fractionation (IsoF) during the liquid-gas phase transition in dilute asymmetric nuclear matter is studied as a function of nucleon momentum. Within a self-consistent thermal model it is shown that the neutron/proton ratio of the gas phase becomes {\it smaller} than that of the liquid phase for energetic nucleons, although the gas phase is overall more neutron-rich. Clear indications of the differential IsoF consistent with the thermal model predictions are demonstrated within a transport model for heavy-ion reactions. Future comparisons with experimental data will allow us to extract critical information about the momentum dependence of the isovector strong interaction.Comment: Rapid Communication, Phys. Rev. C (2007) in pres

Transversus abdominis plane block reduces remifentanil and propofol consumption, evaluated by closed-loop titration guided by bispectral index.

The present prospective, randomized, double-blind study aimed to determine the impact of transversus abdominis plane (TAP) block on propofol and remifentanil consumption, when administered by closed-loop titration guided by processed electroencephalography, i.e., bispectral index (BIS) values. Following institutional review board approval, 60 patients were scheduled for laparoscopic colectomy under general anesthesia. Patients were randomly assigned to receive bilateral TAP block with 20 ml 0.375% ropivacaine (TAP group) or 20 ml 0.9% saline [control (CON) group]. General anesthesia was maintained with propofol and remifentanil administration using closed-loop titration guided by BIS values. The primary outcome was perioperative propofol and remifentanil consumption. The secondary outcomes were hypertensive or hypotensive events requiring treatment, recovery time in PACU and time to first rescue analgesia following surgery. A total of 58 patients participated in the present study. At similar depths of anesthesia, as measured by BIS during the maintenance phase (45-55), patients who received TAP blocks required less propofol (4.2±1.3 vs. 5.5±1.6 mg/kg/h; P<0.001) and remifentanil (0.16±0.05 vs. 0.21±0.05 µg/kg/min; P<0.001). Time to extubation was significantly shorter in the TAP group (9.8±3.2 min) than in the CON group (14.2±4.9 min) (P<0.05). The requirement to treat hemodynamic change was also significantly lower (P<0.05). Pain score at 2 h after surgery was also significantly reduced in the TAP group compared with the CON group (P<0.05), whereas the time to first rescue analgesia was delayed in patients who received TAP block (P<0.05). Postoperative nausea and vomiting occurred at comparable rates in each group (P>0.05). In conclusion, TAP block combined with general anesthesia reduced propofol and remifentanil consumption, shortened time to tracheal extubation and promoted hemodynamic stability in laparoscopic colectomy

NUCLEAR CONSTRAINTS ON PROPERTIES OF NEUTRON STAR CRUSTS

The transition density $\rho_{t}$ and pressure $P_{t}$ at the inner edge separating the liquid core from the solid crust of neutron stars are systematically studied using a modified Gogny (MDI) and 47 popular Skyrme interactions within well established dynamical and thermodynamical methods. It is shown that the widely used parabolic approximation to the full Equation of State (EOS) of isospin asymmetric nuclear matter may lead to huge errors in estimating the \rho_{t} and P_{t}, especially for stiffer symmetry energy functionals $E_{sym}(\rho)$. The \rho_{t} and P_{t} decrease roughly linearly with the increasing slope parameter $L$ of the $E_{sym}(\rho)$ using the full EOS within both methods. It is also shown that the thickness, fractional mass and moment of inertia of neutron star crust are all very sensitive to the parameter $L$ through the $\rho_{t}$. Moreover, it is shown that the $E_{sym}(\rho)$ constrained in the same sub-saturation density range as the neutron star crust by the isospin diffusion data in heavy-ion collisions at intermediate energies limits the transition density and pressure to 0.040 fm^-3}< \rho_{t} < 0.065 fm^-3 and 0.01 MeV/fm^3 < P_{t} < 0.26$MeV/fm^3, respectively. These constrained values for the transition density and pressure are significantly lower than their fiducial values currently used in the literature. Furthermore, the mass-radius relation and several other properties closely related to the neutron star crust are studied by using the MDI interaction. It is found that the newly constrained$\rho_t$and$P_t$together with the earlier estimate of$\Delta I/I>0.014$for the crustal fraction of the moment of inertia of the Vela pulsar impose a stringent constraint of R>= 4.7+4.0M/M_sun km for the radius$R$and mass$M$ of neutron stars.Comment: 55 pages, 20 figures, 2 tables, new results and discussions added, accepted version to appear in Ap

Transcriptional Regulations on the Low-Temperature-Induced Floral Transition in an Orchidaceae Species, Dendrobium nobile: An Expressed Sequence Tags Analysis

Vernalization-induced flowering is a cold-relevant adaptation in many species, but little is known about the genetic basis behind in Orchidaceae species. Here, we reported a collection of 15017 expressed sequence tags (ESTs) from the vernalized axillary buds of an Orchidaceae species, Dendrobium nobile, which were assembled for 9616 unique gene clusters. Functional enrichment analysis showed that genes in relation to the responses to stresses, especially in the form of low temperatures, and those involving in protein biosynthesis and chromatin assembly were significantly overrepresented during 40 days of vernalization. Additionally, a total of 59 putative flowering-relevant genes were recognized, including those homologous to known key players in vernalization pathways in temperate cereals or Arabidopsis, such as cereal VRN1, FT/VRN3, and Arabidopsis AGL19. Results from this study suggest that the networks regulating vernalization-induced floral transition are conserved, but just in a part, in D. nobile, temperate cereals, and Arabidopsis