Representative images of GFP expression in the mouse cornea injected with different serotypes under stereoscopic microscope at day 3, 7, 14, 21 and 28 after injection.

<p>Images a-e indicates PBS group, f-j indicates AAV2 group, k-o indicates scAAV2 group, p-t indicates AAV8 group, u-y indicates Anc80L65 group. * indicates that images at day 3 post-injection taken under bright illumination.</p

IOP changes of mice injected with PBS, AAV2, scAAV2, AAV8 and Anc80L65 before injection and at different time points after injection.

<p>IOP changes of mice injected with PBS, AAV2, scAAV2, AAV8 and Anc80L65 before injection and at different time points after injection.</p

Representative images of GFP expression in the cornea stroma and endothelial cells of mice injected with different serotypes under confocal microscopy 28 days after injection.

<p>Nuclei were stained with DAPI (blue).</p

Superoleophilic and Flexible Thermoplastic Polymer Nanofiber Aerogels for Removal of Oils and Organic Solvents

Chemical cross-linked poly­(vinyl alcohol-<i>co</i>-ethylene) (EVOH) nanofiber aerogels (NFAs) were fabricated employing an economical and facile freeze-drying process. The manufactured chemical cross-linking nanofiber aerogel was successfully confirmed by scanning electron microscopy, attenuated total reflection-Fourier transform infrared spectrometer, and X-ray diffraction. The resulting aerogels showed high porosity (>99%), superior elasticity, elastic durability, high hydrophobicity, and superoleophilicity without any other hydrophobic modification. The cross-linked EVOH NFAs exhibited excellent absorption capacity (ranging from 45 to 102 times their own weight) when exposed to various oils and organic solvents, which was observed to be higher than that for most sorbents reported in the literature. Consequently, it is envisaged that the cross-linked EVOH NFA would play an important role in many fields of pollution removal

Representative images of GFP expression in the HEK293 and HTM cells infected with PBS, AAV2, scAAV2, AAV8 and Anc80L65 with a MOI of 1×10⁴ GC/cell (a-t).

<p>Images were taken under digital inverted microscope at 1 and 3 days after infection (10x magnification). The scale bar is 400 μm. GFP positive cell percentage of HEK293 and HTM cells 1 and 3 days after infection(u-v). Means and standard deviations of three independent experiments are shown (* = p<0.05, ** = p<0.01, *** = p<0.001).</p

Representative images of GFP expression in the trabecular meshwork and ciliary body of mice injection with different serotypes under confocal microscopy 28 days after injection.

<p>The mouse anterior chamber angle tissue was stained with thrombospondin-1(red). Nuclei were stained with DAPI (blue). C indicates cornea, I indicates iris, CB indicates ciliary body, TM indicates trabecular meshwork, SC indicates schlemm’s canal.</p

A series of Keggin-based Ag^I-belt/cycle structures constructed from 5-phenyl-1H-tetrazole and its derivative through Ag–N and Ag–C bonds

<p>Three Keggin-based compounds containing Ag^I belts and cycles constructed from 5-phenyl-1H-tetrazole (<b>L</b>^<b>1</b>) and its derivative 5-m-tolyl-1H-tetrazole (<b>L</b>^<b>2</b>), [Ag₉<b>L</b>^<b>1</b>₅(PW^VW^VI₁₁O₄₀)]·H₂O (<b>1</b>), [Ag₁₁<b>L</b>^<b>1</b>₆(H₂O)₂(SiMo^VMo^VI₁₁O₄₀)] (<b>2</b>) and [Ag₁₀<b>L</b>^<b>2</b>₈(HPMo₁₂O₄₀)]·H₂O (<b>3</b>), have been synthesized under hydrothermal conditions and characterized by IR spectra and single crystal X-ray diffraction. Compound <b>1</b> shows a channel-like 3-D metal-organic framework with Keggin anions in channels. Adjacent layers of <b>2</b> share the same anions to construct a 3-D framework. Compound <b>3</b> has a 2-D metal-organic layer containing Ag^I cycles. Adjacent layers link through sharing Ag–N bonds and a channel-like 3-D framework is formed. Electrochemical and photocatalytic properties of <b>1</b>–<b>3</b> have been studied. The experimental results show that <b>1</b>–<b>3</b> have excellent catalytic performance for reduction of nitrite and bromate and also have photocatalytic properties for degradation of MB and RhB.</p

Quantitative assessment of cone photoreceptor transduction across the nonhuman primate retina.

<p>For each eye and for each of the foveal, parafoveal, perifoveal and peripheral retina regions, GFP positive cones were counted on histological sections. Shown is the percentage of the average number of GFP-positive cones relative to the average total cone number for each eye and region. Each eye for which viable sections were available was included in this analysis, including those for which the injection was suboptimal or problematic and the bleb may not have extended across the fovea/parafovea.</p

Quantitative analysis of tropism and transgene expression levels in the NHP eye.

<p>Cynomolgus macaques were injected with AAVs expressing 10⁹ or 10¹⁰ GC per eye. Following necropsy at 4–5 month post injection, retinas were sectioned and analyzed for direct fluorescence. Data from a morphometric analysis in the RPE (A) and PR (B) layers is presented with the relative area of transduction on the left and an intensity scoring on the right. AAV2 and AAV8 data are historical data from an analogous, previously reported study <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053463#pone.0053463-Vandenberghe1" target="_blank">[6]</a> [a 10⁹ GC injection was not performed for AAV2 (n/a)]. Eyes for which the injection failed as noted in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053463#pone-0053463-t001" target="_blank">Table 1</a> were excluded from this analysis. Data is presented as average and standard deviation.</p

Design, surgical and ophthalmoscopic observations.

<p>Cells with italicized font are excluded from the quantitative post mortem analysis shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053463#pone-0053463-g001" target="_blank">Fig. 1</a> due to injection issues as highlighted in the notes column; % SR and IV reflect estimates of vector deposit in subretinal and vitreal spaces respectively; GFP Score ranges from 0 (no visible expression) to 4 (broad and intense GFP) and reflects a subjective composite of both intensity and area of transduction as observed by indirect ophthalmoscopy; F: foveal; B: blood; A: visible air bubble; 2x: injection procedure was interrupted following sclerotomy and restarted making use of the initial sclerotomy a second time due to technical concerns; SRPE: subRPE or choroidal injection; JFL: juxtafoveal; M: macular hole; SR: subretinal; IV: intravitreal; ID: identification number; GFP: green fluorescent protein; OD: optic disc.</p