NMR Structures of Apo L. casei Dihydrofolate Reductase and Its Complexes with Trimethoprim and NADPH: Contributions to Positive Cooperative Binding from Ligand-Induced Refolding, Conformational Changes, and Interligand Hydrophobic Interactions

bS Supporting Information The enzyme dihydrofolate reductase (DHFR; 5,6,7,8-tetra-hydrofolate:NADPH oxidoreductase, EC 1.5.1.3) catalyzes the reduction of 7,8-dihydrofolate (DHF) to 5,6,7,8-tetrahydro-folate (THF) using NADPH as coenzyme.1 Since THF and its metabolites are precursors of purine and pyrimidine bases, the normal functioning of this enzyme is essential for proliferating cells. This makes DHFR an excellent target for antifolate drugs such as methotrexate (anticancer), pyrimethamine (antimalarial), and trimethoprim (antibacterial). Such agents act by inhibiting the enzyme in parasitic or malignant cells.1,2 The cooperative binding of ligands to DHFR plays an important role not only in the enzyme catalytic cycle (negative cooperativity in THF/ NADPH binding)3 but also in enzyme inhibition (positive cooperativity in antifolate/NADPH binding).4 The effects of positive cooperative binding in controlling enzyme inhibition ar

The importance of active foreign language competence – Maximising choice for graduate translators

This paper will discuss why active foreign language competence is an essential skill that universities must teach translation students to ensure that they can meet the market needs of today and tomorrow. Commencing with a discussion of the native speaker principle and the traditional arguments against translating into the non-native language, this paper will critically examine the native speaker principle, its assumptions and consequences before going on to explain why the academic ideal, propagated particularly at Anglophone universities, of the target language native-speaking translator often does not, for many reasons, fit with what clients really want and need. The paper will conclude by illustrating additional benefits of excellent foreign language competence, aside from the question of translating into the non-native language, to ensure that graduates are equipped to master the many additional skills they must acquire in order to be competitive in the market and to maximise their flexibility and adaptability in an ever-changing industry

Kindertotenlieder

Nun will die Sonn' so hell aufgeh'n.--Nun seh' ich wohl, warum so dunkel Flammen.--Wenn dein Mütterlein.--Oft denk' ich, sie sind nur ausgegangen.--In diesem Wetter

Aus dem Schi-King. Selections

score (77 p.) 30 cm. For high voice and piano

Gedichte von Friedrich Rückert. English & German

Songs (High voice) with piano. For high voice and piano. Text in German and English. v.1. Ein Obdach gegen Sturm und Regen.--v.2. Gestern war ich Atlas.--v.3. Die sieben Siegel.--v.4. Morgenrot.--v.5. Ich sehe wie in einem Spiegel

Um Mitternacht / Gustav Mahler ; [Gedicht von] Rückert.

1 vocal score (7 p.) ; 33 cm. For high voice and piano; acc. originally for orchestra

Gedichte von Friedrich Rückert. English & German

5 v. 34 cm. For high voice and piano. Text in German and English. v.1. Ein Obdach gegen Sturm und Regen.--v.2. Gestern war ich Atlas.--v.3. Die sieben Siegel.--v.4. Morgenrot.--v.5. Ich sehe wie in einem Spiegel

Roland zu Bremen und andre Gedichte von Friedrich Rückert : für eine Singstimme mit Klavierbegleitung, op. 64 / gesetzt von Christian Sinding.

1 score (26 p.) ; 33 cm. In binder’s collection. Roland zu Bremen Beschwichtigter Zweifel Thöricht, wer im Paradies kann wohnen Schmachtend vor Liebe Götter! keine frostige Ewigkeit Und wenn in ihrem Liebesglanz Erlischt die letzte Rose Meine Töne, still und heiter, Zu den Liebsten steigt hinan! Triolet Am Rhein und am Main und am Neckar ist’s schön Gieb den Kuss mir nir nur heute; Ob Du morgen es kannst, wer weiss

Gedichte von Friedrich Rückert. English & German

5 v. 34 cm. For high voice and piano. Text in German and English. v.1. Ein Obdach gegen Sturm und Regen.--v.2. Gestern war ich Atlas.--v.3. Die sieben Siegel.--v.4. Morgenrot.--v.5. Ich sehe wie in einem Spiegel

Gedichte von Friedrich Rückert. English & German

5 v. 34 cm. For high voice and piano. Text in German and English. v.1. Ein Obdach gegen Sturm und Regen.--v.2. Gestern war ich Atlas.--v.3. Die sieben Siegel.--v.4. Morgenrot.--v.5. Ich sehe wie in einem Spiegel