3 research outputs found
Comparative Analysis by Polymerase Chain Reaction Amplified Minicircles of Kinetoplast DNA of a Stable Strain of Trypanosoma cruzi from São Felipe, Bahia, its Clones and Subclones: Possibility of Predominance of a Principal Clone in this Area
Comparative Analysis by Polymerase Chain Reaction Amplified Minicircles of Kinetoplast DNA of a Stable Strain of Trypanosoma cruzi from São Felipe, Bahia, its Clones and Subclones: Possibility of Predominance of a Principal Clone in this Area
Molecular characterization of one stable strain of Trypanosoma cruzi,
the 21 SF, representative of the pattern of strains isolated from the
endemic area of São Felipe, State of Bahia, Brazil, maintained for
15 years in laboratory by serial passages in mice and classified as
biodeme Type II and zymodeme 2 has been investigated. The kinetoplast
DNA (kDNA) of parental strain, 5 clones and 14 subclones were analyzed.
Schizodeme was established by comparative study of the fragments
obtained from digestion of the 330-bp fragments amplified by polymerase
chain reaction (PCR) from the variable regions of the minicicles, and
digested by restriction endonucleases Rsa I and Hinf I. Our results
show a high percentual of similarity between the restriction fragment
lenght polymorphism (RFLP) for the parental strain and its clones and
among these individual clones and their subclones at a level of 80 to
100%.This homology indicates a predominance of the same "principal
clone" in the 21SF strain and confirms the homogeneity previously
observed at biological and isozymic analysis. These results suggest the
possibility that the T. cruzi strains with similar biological and
isoenzymic patterns, circulating in this endemic area, are
representative of one dominant clone. The presence of "principal
clones" could be responsible for a predominant tropism of the parasites
for specific organs and tissues and this could contribute to the
pattern of clinico-pathological manifestations of Chagas's disease in
one geographical area
Comparative Analysis by Polymerase Chain Reaction Amplified Minicircles of Kinetoplast DNA of a Stable Strain of Trypanosoma cruzi from São Felipe, Bahia, its Clones and Subclones: Possibility of Predominance of a Principal Clone in this Area
Molecular characterization of one stable strain of Trypanosoma cruzi,
the 21 SF, representative of the pattern of strains isolated from the
endemic area of São Felipe, State of Bahia, Brazil, maintained for
15 years in laboratory by serial passages in mice and classified as
biodeme Type II and zymodeme 2 has been investigated. The kinetoplast
DNA (kDNA) of parental strain, 5 clones and 14 subclones were analyzed.
Schizodeme was established by comparative study of the fragments
obtained from digestion of the 330-bp fragments amplified by polymerase
chain reaction (PCR) from the variable regions of the minicicles, and
digested by restriction endonucleases Rsa I and Hinf I. Our results
show a high percentual of similarity between the restriction fragment
lenght polymorphism (RFLP) for the parental strain and its clones and
among these individual clones and their subclones at a level of 80 to
100%.This homology indicates a predominance of the same "principal
clone" in the 21SF strain and confirms the homogeneity previously
observed at biological and isozymic analysis. These results suggest the
possibility that the T. cruzi strains with similar biological and
isoenzymic patterns, circulating in this endemic area, are
representative of one dominant clone. The presence of "principal
clones" could be responsible for a predominant tropism of the parasites
for specific organs and tissues and this could contribute to the
pattern of clinico-pathological manifestations of Chagas's disease in
one geographical area