ECONOMIC, ENVIRONMENTAL AND ENERGY USE IMPLICATIONS OF SHORT-SEASON COTTON PRODUCTION: TEXAS LOWER RIO GRANDE VALLEY

Crop Production/Industries,

Interactions between Upf1 and the Decapping Factors Edc3 and Pat1 in Saccharomyces cerevisiae

In Saccharomyces cerevisiae, mRNA transcripts with premature termination codons are targeted for deadenylation independent decapping and 5′ to 3′ decay in a quality control pathway termed nonsense-mediated decay (NMD). Critical factors in NMD include Upf1, Upf2, and Upf3, as well as the decapping enzyme, Dcp2/Dcp1. Loss of Upf2 or Upf3 leads to the accumulation of not only Upf1 and Dcp2 in P-bodies, but also of the decapping-activators Pat1, Dhh1, and Lsm1. An interaction between Upf1 and Dcp2 has been identified, which might recruit Dcp2 to the NMD decapping complex. To determine the nature and significance of the Dcp2-Upf1 interaction, we utilized the yeast two-hybrid assay to assess Upf1 interactions with various mRNA decapping factors. We find that although Dcp2 can interact with Upf1, this interaction is indirect and is largely dependent on the Edc3 protein, which interacts with the N-terminal domain of Upf1 at an overlapping, but not identical, site as Upf2. We also found that Pat1 has an independent two-hybrid interaction with the N-terminus of Upf1. Assessment of both reporter and endogenous NMD transcripts suggest that the decapping stimulators, including Edc3 and Pat1, as well as Edc1 and Edc2, are not essential for NMD under normal conditions. This work defines a larger decapping complex involved in NMD, but indicates that components of that complex are not required for general NMD and might either regulate a subset of NMD transcripts or be essential for proper NMD under different environmental conditions

Symbolic Magnifying Lens Abstraction in Markov Decision Processes

In this paper, we combine abstraction-refinement and symbolic techniques to fight the state-space explosion problem when model checking Markov decision processes (MDPs). The abstract-refinement technique, called "magnifying-lens abstraction" (MLA), partitions the state-space into regions and computes upper and lower bounds for reachability and safety properties on the regions, rather than the states. To compute such bounds, MLA iterates over the regions, analyzing the concrete states of each region in turn - as if one was sliding a magnifying lens across the system to view the states. The algorithm adaptively refines the regions, using smaller regions where more detail is required, until the difference between the bounds is below a specified accuracy. The symbolic technique is based on multi-terminal binary decision diagrams (MTBDDs) which have been used extensively to provide compact encodings of probabilistic models. We introduce a symbolic version of the MLA algorithm, called "symbolic MLA", which combines the power of both practical techniques when verifying MDPs. An implementation of symbolic MLA in the probabilistic model checker PRISM and experimental results to illustrate the advantages of our approach are presented

Keys to Canola Production in South Texas

4 pp.Canola is a term patented by the Canadian government. It refers to rapeseed (Brassica napus), which has specific chemical properties that lend themselves to the production of a high-quality cooking oil and an edible meal used in livestock rations. This publication discusses the growing, marketing and processing of canola

Neonicotinoid Insecticides Alter Induced Defenses and Increase Susceptibility to Spider Mites in Distantly Related Crop Plants

Chemical suppression of arthropod herbivores is the most common approach to plant protection. Insecticides, however, can cause unintended, adverse consequences for non-target organisms. Previous studies focused on the effects of pesticides on target and non-target pests, predatory arthropods, and concomitant ecological disruptions. Little research, however, has focused on the direct effects of insecticides on plants. Here we demonstrate that applications of neonicotinoid insecticides, one of the most important insecticide classes worldwide, suppress expression of important plant defense genes, alter levels of phytohormones involved in plant defense, and decrease plant resistance to unsusceptible herbivores, spider mites Tetranychus urticae (Acari: Tetranychidae), in multiple, distantly related crop plants.Using cotton (Gossypium hirsutum), corn (Zea mays) and tomato (Solanum lycopersicum) plants, we show that transcription of phenylalanine ammonia lyase, coenzyme A ligase, trypsin protease inhibitor and chitinase are suppressed and concentrations of the phytohormone OPDA and salicylic acid were altered by neonicotinoid insecticides. Consequently, the population growth of spider mites increased from 30% to over 100% on neonicotinoid-treated plants in the greenhouse and by nearly 200% in the field experiment.Our findings are important because applications of neonicotinoid insecticides have been associated with outbreaks of spider mites in several unrelated plant species. More importantly, this is the first study to document insecticide-mediated disruption of plant defenses and link it to increased population growth of a non-target herbivore. This study adds to growing evidence that bioactive agrochemicals can have unanticipated ecological effects and suggests that the direct effects of insecticides on plant defenses should be considered when the ecological costs of insecticides are evaluated

A second-generation ligand for the enantioselective rhodium-catalyzed addition of arylboronic acids to alkenylazaarenes

A 2,4,6-trialkylanilide-containing chiral diene has been identified as a superior ligand for the enantioselective rhodiumcatalyzed arylation of alkenylazaarenes with arylboronic acids

Keys to Canola Production in South Texas

4 pp.Canola is a term patented by the Canadian government. It refers to rapeseed (Brassica napus), which has specific chemical properties that lend themselves to the production of a high-quality cooking oil and an edible meal used in livestock rations. This publication discusses the growing, marketing and processing of canola

Managing Insects and Mite Pests of Texas Sorghum

32 pp., 22 tables, 3 figures, 30 color photos, 20 drawingsThis is the complete guide for Texas sorghum growers. It covers an integrated approach to managing insect and mite pests to help growers prevent, diagnose and control damaging infestations. This publication offers suggestions for managing 26 insect and mite pests of sorghum

Is neuroblastoma screening evaluation needed and feasible?

Despite the five million children who have been screened for neuroblastoma in Japan through detection of catecholamine metabolites, it is still uncertain whether screening for this disease is beneficial. The Japanese study has clearly indicated that screening at 6 months or earlier leads to heavy overdiagnosis. It is shown in this paper that screening at a later age may give the same reduction in mortality with possibly less overdiagnosis. However, it is estimated that, even with two screens at 12 and 18 months, the reduction in mortality would not greatly exceed 25%, under realistic hypotheses on the length of the preclinical phase of the disease. The evaluation of the efficacy of this screening strategy would need the recruitment of half a million children per year over 5-7 years and the follow-up of an equal number of controls. Such a trial would improve our knowledge of the natural history of the disease and might help to answer some questions raised recently regarding its biological heterogeneity