57 research outputs found
Products of cultured neuroglial cells: II. The production of fibronectin by C6 glioma cells
The possibility of fibronectin production by C6 glioma cells was examined with assays which require protein synthesis. Proteins produced by C6 cells using radiolabeled amino acid precursors were tested for affinity to collagen by binding to immobilized gelatin. The predominant collagen binding protein made by C6 coelectrophoresed with fibronectin synthesized by control fibroblasts and with the larger of the two proteins in unlabeled fibronectin when applied to polyacrylamide gels with sodium dodecyl sulfate (SDS). In addition, C6 produced a larger collagen binding protein of approximately 270,000 molecular weight. Solubilities in urea solutions of the collagen-binding proteins made by C6 cells and fibroblasts were similar. Immunofluorescence showed fibronectin associated with the C6 cell monolayer, but less abundant than the fibronectin associated with fibroblasts. Results provide evidence for the production of fibronectin by the C6 glioma cell line.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/45400/1/11064_2004_Article_BF00964399.pd
Characterization and use of an allotype-specific monoclonal antibody to placental alkaline phosphatase in the study of cancer-related phosphatase polymorphism.
Alpha-Fetoprotein on Human Peripheral Blood Lymphocytes Does Not Block Complement-dependent Lymphocytotoxicity
Homology between the primary structure of α-fetoprotein, deduced from a complete cDNA sequence, and serum albumin
Improved Diagnosis of Neural Tube Defects: Measurement of Concanavalin A-Reactive Variants of AFP in Amniotic Fluid
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