MiR-205-5p inhibition by locked nucleic acids impairs metastatic potential of breast cancer cells

Mir-205 plays an important role in epithelial biogenesis and in mammary gland development but its role in cancer still remains controversial depending on the specific cellular context and target genes. We have previously reported that miR-205-5p is upregulated in breast cancer stem cells targeting ERBB pathway and leading to targeted therapy resistance. Here we show that miR-205-5p regulates tumorigenic properties of breast cancer cells, as well as epithelial to mesenchymal transition. Silencing this miRNA in breast cancer results in reduced tumor growth and metastatic spreading in mouse models. Moreover, we show that miR-205-5p knock-down can be obtained with the use of specific locked nucleic acids oligonucleotides in vivo suggesting a future potential use of this approach in therapy

Breast metastasis and lung large-cell neuroendocrine carcinoma: first clinical observation

The lung Large-cell neuroendocrine carcinoma (LCNEC) is a very rare aggressive neuroendocrine tumor with a high propensy to metastasize and very poor prognosis. We report an atypical presentation of lung large-cell neuroendocrine carcinoma was diagnosed from a metastatic nodule on the breast. Our patient is a 59 years-old woman that presented in March 2014 non productive cough. A CT scan showed multiple brain, lung, adrenal gland and liver secondary lesions; moreover, it revealed a breast right nodule near the chest measuring 1.8 cm. The breast nodule and a lung lesions were biopsied and their histology and molecular diagnosis were LCNEC of the lung. To our knowledge, this is the first documented case of breast metastasis from LCNEC of the lung. Furthermore, breast metastasis from extramammary malignancy is uncommon and its diagnosis is difficult but important for proper management and prediction of prognosis. Therefore, a careful clinical history with a thorough clinical examination is needed to make the correct diagnosis. Moreover, metastasis to the breast should be considered in any patient with a known primary malignant tumor history who presents with a breast lump. Anyhow, pathological examination should be performed to differentiate the primary breast cancer from metastatic tumor. Therefore, an accurate diagnosis of breast metastases may not only avoid unnecessary breast resection, more importantly it is crucial to determine an appropriate and systemic treatment

The PS 10 MHz High Level RF System Upgrade

In view of the upgrade of the injectors for the High Luminosity LHC, significantly higher bunch intensity is required for LHC-type beams. In this context an upgrade of the main accelerating RF system of the Proton Synchrotron (PS) is necessary, aiming at reducing the cavity impedance which is the source of longitudinal coupled-bunch oscillations. These instabilities pose as a major limitation for the increase of the beam intensity as planned after LS2. The 10 MHz RF system consists in 11 ferrite loaded cavities, driven by tube-based power amplifiers for reasons of radiation hardness. The cavity-amplifier system is equipped with a wide-band feedback that reduces the beam induced voltage. A further reduction of the beam loading is foreseen by upgrading the feedback system, which can be reasonably achieved by increasing the loop gain of the existing amplification chain. This paper describes the progress of the design of the upgraded feedback system and shows the results of the tests on the new amplifier prototype, installed in the PS during the 2015-16 technical stop. It also reports the first results of its performance with beam, observed in the beginning of the 2016 run

Kaon oscillations in the Standard Model and Beyond using Nf=2 dynamical quarks

We compute non-perturbatively the B-parameters of the complete basis of four-fermion operators needed to study the Kaon oscillations in the SM and in its supersymmetric extension. We perform numerical simulations with two dynamical maximally twisted sea quarks at three values of the lattice spacing on configurations generated by the ETMC. Unwanted operator mixings and O(a) discretization effects are removed by discretizing the valence quarks with a suitable Osterwalder-Seiler variant of the Twisted Mass action. Operators are renormalized non-perturbatively in the RI/MOM scheme. Our preliminary result for BK(RGI) is 0.73(3)(3).Comment: 7 pages, 3 figures, 1 table, proceedings of the XXVII Int'l Symposyum on Lattice Field Theory (LAT2009), July 26-31 2009, Peking University, Beijing (China