Teknik Isolasi dan Elektroforesis Dna Total pada Kryptopterus Apogon (Bleeker 1851) dari Sungai Kampar Kiri dan Tapung Hilir Kabupaten Kampar Provinsi Riau

Kryptopterus apogon is one of freshwater fish species that was only found in floodplain river in Riau Province. Many species of lais fish are found in Riau Province rivers and morphologically has the same shape therefore it is necessary to find alternative markers to distinguish the species. The important step in genetic analysis is DNA isolation. This study aimed to isolate the total DNA from Kryptopterus apogon. The fish samples were taken from the Kampar Kiri and Tapung Hilir rivers. Isolation of total DNA was done by taking the muscle tissue from the fish tail (1 mm x 1 mm in size). Isolation of total DNA was done using DNA isolation kit (QIAGEN DNeasy Blood & Tissue). After that, the total DNA was checked on 1% agarose gel to estimate the quantity and quality of total DNA. Total isolated DNA of Kryptopterus apogon from two rivers had various quality and quantity. Tha total quantity of DNA molecules ranged from to 100-200 ng/μl, while the total quality was good enough or not fragmented. The DNA was clear, thick, and no smear

Jom Fmipa Volume 1 No. 2 Oktober 291 Pertumbuhan Cacing Tanah (Perionyx SP) Pada Dua Media

This research was conducted to determine the growth of earthworm ( Peryonix sp) in two types of media, that is mixture of palm sheath waste with chicken manures and mixture of vegetables waste with chicken manures. This research was conducted at Biological Garden Department of Biology Math and Science Faculty Riau University. The research used completely randomized design with five treatments and three replications i.e treatments M1 (100% palm sheath waste); M2 (75% palm sheath waste + 25% chicken manure); M3 (50% palm sheath waste + 50% chicken manure); M4 (25% palm sheath waste + 75% chicken manure) and M5 (100% chicken manure), S1 (100% vegetables waste); S2 (75% vegetables waste + 25% chicken manure); S3 (50% vegetables waste + 50% chicken manure); S4 (25% vegetables waste + 75% chicken manure) and S5 (100% chicken manure). Analysis of variance showed that type of media had significantly affected body weight and amount of coccon produced. The biggest body weight and amount coccon were at media M2 and S3 in the day of 20

Mouthpart and digestive tract morphology of the synchronized firefly, Pteroptyx tener (coleoptera: lampyridae)

The firefly, Pteroptyx tener (Coleoptera: Lampyridae) lives in the mangrove area and is economically valuable for ecotourism because of its unique synchronous flashing behavior. It has been observed that the adult species does not have the appearance of sucking type mouthpart morphology though it is believed that its diet depends on the plant sap. This study focused on the morphology of the mouthpart and, anatomy and histology of the digestive tract of P. tener. From gross morphology observation, P. tener has hypognathus mouthpart with a crescent-like narrow apicle mandible. Scanning Transmission Electron Microscope (STEM) image showed that P. tener has a special food canal inside its mandible that runs from the tip to the end part of the mandible. The tip of the mandible could be used as piercing equipment for P. tener to suck plant sap for feeding purposes. Morphological investigations of the digestive tract showed that this species have a small crop, large ventriculus and long intestinal tract that is suitable for a liquid feeder insect. Histological sections show that the digestive tract consist of thick muscle layer especially on the hindgut and thin epithelial layer on all parts of the gut