PLANO TERRITORIAL DE DESENVOLVIMENTO RURAL SUSTENTÁVEL – PTDRS

Neste artigo estará sendo tratada uma questão de grande relevância para o meio rural do Médio Alto Uruguai, do Rio Grande do Sul, pois objetiva relatar e comentar a construção de um Plano Territorial de Desenvolvimento Rural Sustentável - PTDRS definidor das ações que podem contribuir com um bom desempenho da agricultura familiar do referido território. O PTDRS representará um grande instrumento orientador de possíveis atitudes que contribuam com um melhor desenvolvimento deste setor da agricultura regional. O mesmo pode ser entendido como um conjunto organizado de diretrizes, estratégias e compromissos relativos às ações que serão realizadas no futuro, visando ao desenvolvimento sustentável do território, resultante de consensos compartilhados por atores sociais (grupos e segmentos diferenciados da sociedade civil), Estado e Federação, nas decisões tomadas no processo participativo. Também constará neste texto um conjunto de possibilidades que poderão estar nascendo a partir da construção coletiva e propositiva que estarão sendo geradas como indicadores de projetos de desenvolvimento sustentável que contribuirão com uma nova dinâmica desta agricultura familiar. A Universidade Regional Integrada do Alto Uruguai e das Missões-URI Campus de Frederico Westphalen é a entidade executora das ações que, em parceria com entidades regionais, que culminaram com a construção do PTDRS, através de convênio firmado com o Ministério do Desenvolvimento Agrário (MDA) e Secretaria de Desenvolvimento Territorial (SDT).-This article will deal with a question of great relevance for the rural way in the Medium High Uruguay, in the State of Rio Grande do Sul, Brazil, because its aims are to tell and to comment on the construction of a Territorial Plan of Rural Sustainable Development - PTDRS, defining the actions that can contribute for a good development of the family agriculture to the referred territory. The PTDRS will represent a relevant instrument that can contribute to a better development of this branch of regional agriculture. It can also be understood as an organized set of lines of directions relative to strategies and commitments to the actions that will be carried through in the future, aiming at to the sustainable development of the territory as a result of a shared consensus by social actors (groups and diferentiated segments of the civil society), State and Federation, in the decisions taken in the participative process. It is also considered in this text a set of possibilities that may be springing up from the collective and propositive construction that are beinggenerated as indicators of sustainable development projects that will contribute as a new dynamics of this family agriculture. The Integrated Regional University of the Medium High Uruguay and of the Missions - URI, Frederico Westphalen City Campus is the executor entity of the actions that, in partnership with regional entities have culminated in the construction of the PTDRS, through an agreement with the Ministry of the Agrarian Development (MDA), and the Secretary of Territorial Development (SDT)

OXavidin for Tissue Targeting Biotinylated Therapeutics

Avidin is a glycoprotein from hen egg white that binds biotin with very high affinity. Here we describe OXavidin, a product containing aldehyde groups, obtained by ligand-assisted sugar oxidation of avidin by sodium periodate. OXavidin chemically reacts with cellular and tissue proteins through Schiff's base formation thus residing in tissues for weeks while preserving the biotin binding capacity. The long tissue residence of OXavidin as well as that of OXavidin/biotinylated agent complex occurs in normal and neoplastic tissues and immunohistochemistry shows a strong and homogenous stromal localization. Once localized in tissue/tumor, OXavidin becomes an “artificial receptor” for intravenous injected biotin allowing tumor targeting with biotinylated therapeutics like radioisotopes or toxins. Moreover, present data also suggest that OXavidin might be useful for the homing of biotinylated cells. Overall, OXavidin exhibits a remarkable potential for many different therapeutic applications

Pilonidal sinus disease. Preliminary case-control study on heat-related wound dehiscence

Background: Pilonidal disease is a morbid condition of the young population, that could impair quality of life with a high cost for the health care system. No consensus exists on optimal surgical treatment, even if several techniques have been proposed. In this preliminary case-control study we compared excision by knife and diathermy to investigate if wound dehiscence could be related to heat spreading during excision of the sinus. Materials and method: Between January 2017 and February 2018, 29 patients underwent to sinus excision.16 patients underwent sinus excision by diathermy (named "Hot" group, case-group) while 13 patients underwent excision by the knife as the control group (named "Cold" group). The temperature data were recorded for both groups. Were considered primary and secondary outcomes. Results: the cold group has worse outcomes in operative time and blood loss, but better results in post-operative pain at first day and first control, number of weekly and total dressings until healing, time for full wound recovery, days to return to work, patient feeling feedback and scar aspect. Wounds healed within 8-12 days were 84.6% in the Cold group and 18.8% in the Hot one. I° Dindo-Clavien complications were respectively 15.4% and 100.0% for the Cold and Hot group. No differences were recorded for II° Dindo-Clavien complications and in days of hospitalization. Conclusion: cold excision of the sinus pilonidalis has better results both in terms of precarious healing and quality of life, probably because the tissues are not subjected to diathermocoagulation damage and therefore the healing occurs more quickly. (United States National Institutes of Health, www.clinicaltrial.gov, number NCT03764657, www.researchregistry.com UIN 5003)

Synthesis and preliminary in vitro evaluation of DOTA-Tenatumomab conjugates for theranostic applications in tenascin expressing tumors

Tenatumomab is an anti-tenascin murine monoclonal antibody previously used in clinical trials for delivering radionuclides to tumors by both pre-targeting (biotinylated Tenatumomab within PAGRIT) and direct 131Iodine labeling approaches. Here we present the synthesis and in vitro characterization of three Tenatumomab conjugates to bifunctional chelating agents (NHS-DOTA, NCS-DOTA and NCS-DTPA). Results indicate ST8198AA1 (Tenatumomab-DOTAMA, derived by conjugation of NHS-DOTA), as the most promising candidate in terms of conjugation rate and yield, stability, antigen immunoreactivity and affinity. Labeling efficiency of the different chelators was investigated with a panel of cold metals indicating DOTAMA as the best chelator. Labeling of Tenatumomab-DOTAMA was then optimized with several metals and stability performed confirms suitability of this conjugate for further development. ST8198AA1 represents an improvement of the previous antibody forms because the labeling with radionuclides like 177Lu or 64Cu would allow theranostic applications in patients bearing tenascin expressing tumors

Chemical Linkage to Injected Tissues Is a Distinctive Property of Oxidized Avidin

We recently reported that the oxidized avidin, named AvidinOX®, resides for weeks within injected tissues as a consequence of the formation of Schiff's bases between its aldehyde groups and tissue protein amino groups. We also showed, in a mouse pre-clinical model, the usefulness of AvidinOX for the delivery of radiolabeled biotin to inoperable tumors. Taking into account that AvidinOX is the first oxidized glycoprotein known to chemically link to injected tissues, we tested in the mouse a panel of additional oxidized glycoproteins, with the aim of investigating the phenomenon. We produced oxidized ovalbumin and mannosylated streptavidin which share with avidin glycosylation pattern and tetrameric structure, respectively and found that neither of them linked significantly to cells in vitro nor to injected tissues in vivo, despite the presence of functional aldehyde groups. The study, extended to additional oxidized glycoproteins, showed that the in vivo chemical conjugation is a distinctive property of the oxidized avidin. Relevance of the high cationic charge of avidin into the stable linkage of AvidinOX to tissues is demonstrated as the oxidized acetylated avidin lost the property. Plasmon resonance on matrix proteins and cellular impedance analyses showed in vitro that avidin exhibits a peculiar interaction with proteins and cells that allows the formation of highly stable Schiff's bases, after oxidation