Protein-mediated surface structuring in biomembranes

The lipids and proteins of biomembranes exhibit highly dissimilar conformations, geometrical shapes, amphipathicity, and thermodynamic properties which constrain their two-dimensional molecular packing, electrostatics, and interaction preferences. This causes inevitable development of large local tensions that frequently relax into phase or compositional immiscibility along lateral and transverse planes of the membrane. On the other hand, these effects constitute the very codes that mediate molecular and structural changes determining and controlling the possibilities for enzymatic activity, apposition and recombination in biomembranes. The presence of proteins constitutes a major perturbing factor for the membrane sculpturing both in terms of its surface topography and dynamics. We will focus on some results from our group within this context and summarize some recent evidence for the active involvement of extrinsic (myelin basic protein), integral (Folch-Lees proteolipid protein) and amphitropic (c-Fos and c-Jun) proteins, as well as a membrane-active amphitropic phosphohydrolytic enzyme (neutral sphingomyelinase), in the process of lateral segregation and dynamics of phase domains, sculpturing of the surface topography, and the bi-directional modulation of the membrane biochemical reactivity

Heritage and Sustainability: Regulating Participation

In the past three decades, there has been increasing research carried out on the role of heritage and its processes in achieving broader sustainable development objectives beyond heritage conservation. As part of this movement, people-centered approaches and participation have been widely integrated into international regulations and guidelines on heritage management, stimulating the implementation of case studies-based research worldwide. Despite the wide advocacy of participatory heritage practices’ contributions to more inclusive and culturally sensitive local development in a great variety of projects, there is limited research into the roles these practices can have in addressing sustainability objectives. How are these roles addressed in international heritage regulatory frameworks, and what forms of participation are promoted for their fulfillment? This paper seeks to answer this research question through a content analysis of international declarations, conventions, guidelines, and policy documents focused on the roles and forms of participation that are promoted. A crossed-matched analysis of results reveals that active forms of participation are those most used to promote all roles and subcategories of participation, as a right, as a driver, and as an enabler of sustainable development. However, fewer active forms are presented as complementary at different stages of sustainability-oriented heritage practices. Moreover, a higher incidence of generic forms of participation can be observed in documents addressing international stakeholders, while partnership and intervention are to be found in those targeting regional and local actors. Nevertheless, the low incidence of decisional forms of participation confirms the challenges of power-sharing at all scales. Trends and influences are highlighted, informing heritage research, governance, and policymaking, but also revealing gaps and ambiguities in current regulations that further research encompassing a larger number of documents might confirm.Heritage & Value