26 research outputs found
Tissue-engineered cartilage framework using lyophilized perichondrium to generate flexible cartilage
An injectable cell-based implant for cartilage repair: physical, morphological, and biomechanical analysis
Activity-directed Isolation of Spasmolytic (anti-cholinergic) Alkaloids from Brugmansia arborea (L.) Lagerheim
Producing a flexible tissue-engineered cartilage framework using expanded polytetrafluoroethylene (ePTFE) membrane as a pseudoperichondrium
BACKGROUND: Both native and engineered cartilage is brittle and fractures easily without perichondrium. The aim of this study was to understand the role of the perichondrium and try to enhance the flexible properties of tissue-engineered cartilage using expanded polytetrafluoroethylene (ePTFE) membrane as a pseudoperichondrium. METHODS: The study was conducted in two phases. In phase I, native swine auricular cartilage of different thicknesses was studied by histologic evaluation and failure testing. Next, isolated perichondrium was bonded to native cartilage slices using fibrin glue or Dermabond and tested to failure. In phase II, swine auricular chondrocytes were suspended in fibrin glue. The chondrocyte-fibrin glue composites were then bound to expanded polytetrafluoroethylene membrane in two trilaminar configurations: In group EC-1, the membrane was in the center, whereas it was on the surfaces in group EC-2. Specimens were implanted into nude mice for 4 weeks, 8 weeks, 12 weeks, and 8 months and subjected to histologic evaluation and failure testing. RESULTS: In phase I, the results demonstrated that perichondrium securely bonded to the cartilage plays an important role in maintaining the flexible nature of elastic cartilage. In phase II, failure testing revealed that specimens in group EC-1 (expanded polytetrafluoroethylene core) were fractured during bending and destroyed after torsion, whereas those in group EC-2 (cartilage core) returned to their original shape without fracturing even after rigorous torsion. Histologic analysis demonstrated that transplanted chondrocytes penetrated into the microporous structure of expanded polytetrafluoroethylene and created a bond to it. CONCLUSION: It is possible to engineer flexible cartilage using expanded polytetrafluoroethylene as a pseudoperichondrium
Improved mass and shape control of tissue engineered cartilage constructs by the addition of TGF-1, IGF-1, and FGF-2 to fibrin glue polymer
Morphological, biomechanical, and biochemical characteristics of tissue engineered cartilage by non-articular chondrocytes for cartilage repair
Tissue engineered cartilage integration to live and devitalized cartilage : a study by reflectance mode confocal microscopy and standard histology
This study investigated the in vivo formation of engineering cartilage within living or devitalized cartilage discs using reflectance mode confocal microscopy and conventional light microscopy. Pig articular chondrocytes were suspended in fibrin glue and placed between two cartilage discs. Four experimental groups were prepared: in groups 1 and 2, the cell-hydrogel composite was placed between two live or between two devitalized cartilage discs, respectively; in groups 3 and 4, acellular fibrin glue was placed between two live or between two devitalized cartilage discs, respectively. Samples were implanted in the back of nude mice and analyzed after 2, 5, and 8 weeks. Results showed that engineered cartilage seems to grow more homogenously when the cell-seeded gel was placed between devitalized cartilages than when it was placed between live cartilage matrices. Confocal microscopy provides valuable information on the integration of tissue-engineered cartilage with native tissue and could be useful for nondestructive imaging in vivo