Simulated pulling experiments of an RNA hairpin

Treballs Finals de Grau de Física, Facultat de Física, Universitat de Barcelona, Curs: 2020, Tutors: Jaime Aspas Cáceres, Fèlix Ritort FarranDifferent studies have shown how to approach the folding process of the RNA, with optical tweezers. On this basis, we've present a simple case of the simulation of a single RNA hairpin pulling experiment. During this work, the experimental device simulated is described and modeled, where also its elastic properties have been considered, as they are an important part of the study. Subsequently, information is extracted from the data and the results obtained on the simulation, related with the thermodynamics of kinetics the unfolding/folding of RNA molecule. The simulated model reproduces qualitatively the experimental results

Thermal Analysis of a MEMS-Based Self-Adaptive Microfluidic Cooling Device

This study presents a thermal analysis of a temperature-driven microfluidic cell through a nonlinear self-adaptive micro valve that provides the mechanisms for the system to maintain a given critical temperature in an efficient way. For the description of the dynamics of the microfluidic cell, a system of two ordinary differential equations subjected to a nonlinear boundary condition, which describes the behavior of the valve, is proposed. The solution of the model, for determined conditions, shows the strong nonlinearity between the overall thermal resistance of the device and the heat flux dissipated due to the action of the thermostatic valve, obtaining a variable thermal resistance from 1.6 × 10−5 to 2.0 × 10−4 Km2/W. In addition, a stability analysis of the temperature-driven microfluidic cell is presented. The stability of the device is essential for its proper functioning and thus, to prevent its oscillating behavior. Therefore, this work focuses on assessing the range of design parameters of the self-adaptive micro valve to produce a stable behavior for the entire system. The stability analysis was performed by studying the linear perturbation around the stationary solution, with the model solved for various heat flows, flow rates, and critical temperatures. Finally, a map of the design parameters space, which specifies the region with asymptotic stability, was found. In this map, the critical temperature (temperature at which the valve initiates the buckling) plays and important role.The research leading to these results was performed within the STREAMS project and received funding from the European Community’s Horizon 2020 program under Grant Agreement N◦ 688564

Early and annual projected savings from anti-CGRP monoclonal antibodies in migraine prevention: a cost-benefit analysis in the working-age population

Anti-CGRP monoclonal antibodies; Migraine prevention; Cost-benefitAnticuerpos monoclonales anti-CGRP; Prevención de la migraña; Costo-beneficioAnticossos monoclonals anti-CGRP; Prevenció de la migranya; Cost-beneficiBackground Migraine is one of the main causes of disability worldwide. Anti-CGRP monoclonal antibodies (MAbs) have proven to be safe and efficacious as preventive migraine treatments. However, their use is restricted in many countries due to their apparently high cost. Cost-benefit studies are needed. Objective To study the cost-benefit of anti-CGRP MAbs in working-age patients with migraine. Methods This is a prospective cohort study of consecutive migraine patients treated with anti-CGRP MAbs (erenumab, fremanezumab and galcanezumab) following National reimbursement policy in a specialized headache clinic. Migraine characteristics and the work impact scale (WPAI) were compared between baseline (M0) and after 3 (M3) and 6 months (M6) of treatment. Using WPAI and the municipal average hourly wage, we calculated indirect costs (absenteeism and presenteeism) at each time point. Direct costs (emergency visits, acute medication use) were also analysed. A cost-benefit study was performed considering the different costs and savings of treating with MAbs. Based on these data an annual projection was conducted. Results From 256 treated working-age patients, 148 were employed (89.2% women; mean age 48.0 ± 8.5 years), of which 41.2% (61/148) were responders (> 50% reduction in monthly headache days (MHD)). Statistically significant reductions between M0 and M3/M6 were found in absenteeism (p < 0.001) and presenteeism (p < 0.001). Average savings in indirect costs per patient at M3 were absenteeism 105.4 euros/month and presenteeism 394.3 euros/month, similar for M6. Considering the monthly cost of anti-CGRP MAbs, the cost-benefit analysis showed savings of 159.8 euros per patient at M3, with an annual projected savings of 639.2 euros/patient. Both responders and partial responders (30–50% reduction in MHD) presented a positive cost-benefit balance. The overall savings of the cohort at M3/M6 compensated the negative cost-benefit balance for non-responders (< 30% reduction in MHD). Conclusion Anti-CGRP MAbs have a positive impact in the workforce significantly reducing absenteeism and presenteeism. In Spain, this benefit overcomes the expenses derived from their use already at 3 months and is potentially sustainable at longer term; also in patients who are only partial responders, prompting reconsideration of current reimbursement criteria and motivating the extension of similar cost-benefit studies in other countries

Impact of DC-DC Converters on the Energy Performance of a Dense Concentrator PV Array under Nonuniform Irradiance and Temperature Profiles

Efficiency losses resulting from electrical mismatching in densely packed photovoltaic arrays present a significant challenge, particularly exacerbated in nonuniformly illuminated receivers and under varying temperatures. Serial configurations are particularly susceptible to radiation nonuniformities, while parallel systems are negatively affected by temperature variations. Various authors have recommended the incorporation of electrical voltage and current sources to mitigate these losses. This study explores different electrical connection configurations utilizing concentrated photovoltaic (CPV) cells and DC-DC electrical current converters. A self-adaptive microfluidic cell matrix cooling system is employed to mitigate thermal dispersion caused by the highly nonuniform illumination profile. The obtained results for each configuration are compared with the total electrical power produced by individual cells, operating under identical radiation and temperature conditions to those of the entire array. The results reveal a noteworthy increase in production across all studied configurations, with the parallel–series arrangement demonstrating the most promising practical utility. This configuration exhibited a remarkable 50.75% increase in power production compared with the standard series connection