Potensi Resistensi Virus HIV-1 terhadap Terapi Anti Retroviral (ART) pada Pasien Voluntary Counseling And Testing (VCT) di Beberapa Kota di Indonesia

Human Immunodeficiency Virus (HIV) is a virus that damages the immune system. The use of antiretroviraltherapy drugs combination (ARV) has demonstrated highly effective in controlling thedevelopment of the HIV virus and prolongs survival. Comprehensive coverage of antiretroviral therapy(ART) also has an impact on the incidence of mutations in the HIV virus that can lead to resistance. Ingeneral, resistance to a particular drug is the result of a mutation in a number of positions in the proteincodinggenes that are targeted by drugs. This study is aimed to find out mutations that related to ARVresistance in some cities in Indonesia. Genotypic assays are used in this study to analyze the viralresistance properties. The samples are from stored biological materials in the Center for Biomedicaland Basic Technology of Health. One hundred and eightspecimens that meet the inclusion criteriawere amplified and sequenced. Further analysis is conducted by uploading sequences to the StanfordUniversity website for the analysis of resistance mutations. Based on the results of this study there aresome mutations in the HIV virus that leads to a low potential of antiretroviral drug resistance of efavirenz(EFV) and nevirapine (NVP). These drugs are used in the first-line treatment of HIV in Indonesia.Overview of the mutations that cause viral resistance to antiretroviral drugs with genetic methods isexpected to serve as baseline data for program development of new drugs or vaccines HIV / AIDS inthe response to HIV in Indonesia