THE INFLUENCE OF FOLIAR FERTILIZERS ON RAPE CROPS FROM SCDA CARACAL

Rapeseed crop fertilization is one of the greatest technological elements that had the most significant impact on production.The quantity and quality of the seed production are determined to a large extent also by the technological link fertilizatio, respectively the amount of fertilizers with macro and micronutrients, the report between the fertilising applied elements and the exact moment of fertilizing. Alongside a basic mineral fertilizer, which remains indispensable, foliar fertilization is a measure to increase both the amount of crop and its quality.Given the importance of fertilization in the technology of rape culture and its requirements for secondary macronutrients and micronutrients, in the present study research had been made on the effect of foliar fertilizers on rape culture at SCDA Caracal.The obtained results led to the conclusion that, when it comes to rape, performing more treatments, as well as combining several fertilizers or treatments, lead to obtaining vigorous plants with an increased number of branches and those of capsules on plants so production will be improved quantitatively and qualitatively

THE POTENTIAL FOR USING MICROORGANISMS IN FOOD BIOTECHNOLOGY

Microorganisms are complex systems, mono or multicellular, with prokaryotic or eukaryotic cells, endowed with their own metabolism and genetic continuity, very diverse in shape, size and metabolic activity. The microorganisms used in biotechnologies are known as useful microorganisms, used in the form of starter cultures for fermentation processes which are the basis for the development of the food industry: fermentative yeasts used in the beer production, wine, ethyl alcohol, bakery; lactic and propionic bacteria used in the dairy industry and in food preservation, etc.The agri-food field is one of the great beneficiaries of the development of biotechnology, being able to provide in the near future most of the raw materials needed for human food. Technological processes for obtaining food, with some exceptions (sugar industry, oil plants industry, milling industry), are biotechnologies that are based on the use of microorganisms or their metabolites

THE VALORIZING OF DIFFERENT WOODY WASTES AS NATURAL SUBSTRATES FOR INTENSIVE CULTIVATION OF MUSHROOMS

This paper presents the results of laboratory experiments regarding the valorizing of different types of lignocellulosic wastes coming from woody species through controlled cultivation of two mushroom species, namely Ganoderma lucidum and Pleurotus ostreatus. Both mushroom species were cultivated in controlled conditions of temperature, humidity, and aeration in order to get their carpophores. The main aim of this work was focused on finding out the best way to convert the woody wastes into useful food products, such as mushroom fruit bodies, by using them as growing sources for the mentioned edible and medicinal mushrooms. The final produced carpophores were weighted and the results were compared to find out the optimal variant to be applied for intensive cultivation of mushrooms

Detection and Quantification of Genetically Modified Soybean in Some Food and Feed Products. A Case Study on Products Available on Romanian Market

The aim of this paper is to trace genetically modified soybean in food and feed products present on the Romanian market by using molecular extraction, identification and quantification methodologies. Nine samples (3 food samples, 5 soybean samples and 1 soybean meal) were analysed using the classical and real-time polymerase chain reaction (PCR) method. DNA-genetically modified organism (GMO) was not detected in two of the three analysed samples (food products). However, it could be found in four samples ranging below the limit of 0.9%, and in three samples, above the limit of 0.9%. The results obtained through real-time PCR quantification show that DNA-RRS was detectable in different amounts in different samples: ranging between 0.27% and 9.36% in soy beans, and reaching 50.98% in soybean meal. The current research focuses on how products containing GMO above the limit (it is common knowledge that it is necessary to label the products containing more than 0.9% Genetically Modified DNA) are differentiated on the market with a view to labeling food and feed products in terms of the accidental presence of approved genetically modified plants. The benefits brought by genetic engineering in obtaining genetically modified organisms can be balanced with their public acceptance and with certain known or unknown risks that they can bring

Determination of the Environmental Pollution Potential of Some Herbicides by the Assessment of Cytotoxic and Genotoxic Effects on Allium cepa

The present study aims to evaluate the potential for the pollution of the environment by two herbicides (quizalofop-p-ethyl and cycloxydim), using the Allium test. The species in question is Allium cepa (onion, 2n = 16), one of the most common plant indicators of environmental pollution. The working method consisted of obtaining the meristematic roots of Allium cepa and their treatment with herbicides at three different concentrations (0.5%, 1%, and 1.5%) for each herbicide for 24 h, for comparison with an untreated control. The results obtained from the cytological study indicated a strong cytotoxic and genotoxic effect for both herbicides, but especially for quizalofop-p-ethyl, where the mitotic index decreased from 30.2% (control) to 9.6% for the variant treated with 1.5% herbicide. In this case, a strong mitodepressive effect was shown by a highly significant percentage (35.4%) of chromosomal aberrations and nuclear alterations: stickiness, fragments, C-mitosis, lobulated nucleus, micronuclei, and nuclear erosion. The mitodepressive effect as well as the percentage of chromosomal aberrations increased with a higher herbicide concentration. The obtained results suggest the strong potential for pollution of the two herbicides, particularly at concentrations higher than 0.5%; therefore, we recommend caution in their use to avoid undesirable effects on the environment

Cytogenetic Study on the Biostimulation Potential of the Aqueous Fruit Extract of Hippophae rhamnoides for a Sustainable Agricultural Ecosystem

This cytogenetic study evaluates the biostimulation potential of the aqueous extract of seabuckthorn fruits (AESF) in plant cells, using the Allium cepa species as a test plant. The effects were monitored both at the macroscopic and microscopically level. The onion bulbs were exposed to the action of different concentrations of AESF (0.5, 1, 1.5, 2, and 2.5%) for 72 h. The obtained results showed the positive effect induced by the aqueous extract on the growth of the meristematic roots, but only at concentrations ranging between 0.5–1.5%, when the average length of the roots had values between 2.51–3.40 cm, which means an increase compared to the untreated control with 3.71–40.49%. Within the same concentration range of the AESF, an effect of intensifying the mitotic activity was recorded. On the other hand, at the 2–2.5% concentration of the AESF, there was an inhibitory effect on the growth of meristematic roots. Additionally, concentrations ≥2% of AESF induced a cytotoxic and genotoxic effect through the occurrence of some chromosomal and nuclear abnormalities in A. cepa cells (sticky, laggards, ring chromosomes, and micronucleus). The obtained results suggest the biostimulation potential of the AESF for plant cells and the possibility of using it as an eco-friendly fertilizer

Cytogenetic Study on the Biostimulation Potential of the Aqueous Fruit Extract of <i>Hippophae rhamnoides</i> for a Sustainable Agricultural Ecosystem

This cytogenetic study evaluates the biostimulation potential of the aqueous extract of seabuckthorn fruits (AESF) in plant cells, using the Allium cepa species as a test plant. The effects were monitored both at the macroscopic and microscopically level. The onion bulbs were exposed to the action of different concentrations of AESF (0.5, 1, 1.5, 2, and 2.5%) for 72 h. The obtained results showed the positive effect induced by the aqueous extract on the growth of the meristematic roots, but only at concentrations ranging between 0.5–1.5%, when the average length of the roots had values between 2.51–3.40 cm, which means an increase compared to the untreated control with 3.71–40.49%. Within the same concentration range of the AESF, an effect of intensifying the mitotic activity was recorded. On the other hand, at the 2–2.5% concentration of the AESF, there was an inhibitory effect on the growth of meristematic roots. Additionally, concentrations ≥2% of AESF induced a cytotoxic and genotoxic effect through the occurrence of some chromosomal and nuclear abnormalities in A. cepa cells (sticky, laggards, ring chromosomes, and micronucleus). The obtained results suggest the biostimulation potential of the AESF for plant cells and the possibility of using it as an eco-friendly fertilizer

The Variability for the Biochemical Indicators at the Winter Wheat Assortment and Identifying the Sources with a High Antioxidant Activity

This study presents the variability of some biochemical indicators in the winter wheat assortments tested in south-western Oltenia (Romania) and identification of the sources showing a high antioxidant activity. The peroxidase activity has intensified as the stress induced by treatment with PEG of different concentrations and in different doses increased. Regarding the peroxidase content, among the varieties treated with PEG 10,000 25%, the majority of the Romanian varieties tested showed higher values of the PEG/control treatment ratio, which suggests tolerance to drought. In reverse, the activity of ascorbate peroxidase is lower in tolerant varieties. The varieties with a subunit report have been noted. Among them are the Izvor variety, known as the drought-tolerant variety, as well as other Romanian varieties: Alex, Delabrad, Lovrin 34, etc. An increased activity of catalase was present in most varieties, so there is the possibility of drought tolerance. Among the varieties highlighted are Romanian varieties (Dropia, Trivale, Nikifor, etc.) but also foreign varieties (Kristina, GH Hattyu, Karlygash, etc.). However, the correlation between yield index in the limited assortment and the antioxidant enzyme content ratios between PEG and control treatments does not exist, suggesting that none of these biochemical indicators are a selective indicator for drought tolerance under the experimental condition

The Variability for the Biochemical Indicators at the Winter Wheat Assortment and Identifying the Sources with a High Antioxidant Activity

This study presents the variability of some biochemical indicators in the winter wheat assortments tested in south-western Oltenia (Romania) and identification of the sources showing a high antioxidant activity. The peroxidase activity has intensified as the stress induced by treatment with PEG of different concentrations and in different doses increased. Regarding the peroxidase content, among the varieties treated with PEG 10,000 25%, the majority of the Romanian varieties tested showed higher values of the PEG/control treatment ratio, which suggests tolerance to drought. In reverse, the activity of ascorbate peroxidase is lower in tolerant varieties. The varieties with a subunit report have been noted. Among them are the Izvor variety, known as the drought-tolerant variety, as well as other Romanian varieties: Alex, Delabrad, Lovrin 34, etc. An increased activity of catalase was present in most varieties, so there is the possibility of drought tolerance. Among the varieties highlighted are Romanian varieties (Dropia, Trivale, Nikifor, etc.) but also foreign varieties (Kristina, GH Hattyu, Karlygash, etc.). However, the correlation between yield index in the limited assortment and the antioxidant enzyme content ratios between PEG and control treatments does not exist, suggesting that none of these biochemical indicators are a selective indicator for drought tolerance under the experimental condition