Number of repeat units of six VNTR loci in four clones obtained from pandemic <i>Vibrio parahaemolyticus</i> strains VpKX and PMC57.5 and mutation rates after prolonged in vitro culturing.

<p>Mutat rate: Mutation rate (x 10⁴).</p

Phylogenetic tree of the mutants from the populations of the four different clones obtained after serial subculturing of pandemic <i>V. parahaemolyticus</i>.

<p>Tree was obtained by MST-Manhattan. The founder of each cluster is indicated above the clusters.</p

Frequency of observed and expected mutants according to a geometric distribution, with 1, 2, 3, or 4 repeat unit changes for each of the clones examined.

<p>The expected frequency of mutants according to geometric distribution is shown in bold italics.</p

Frequency of mutants with increasing differences in the number of repeat units.

<p>Black bars correspond to percent of predicted mutants. Combined bars correspond to percentage of observed mutants, white sections of bars correspond to deletions and gray to insertions.</p

Phylogenetic tree of native isolates of pandemic <i>V. parahaemolyticus</i> obtained worldwide.

<p>Phylogenetic tree generated by MST-Manhattan for native isolates obtained from southern Chile (blue) northern Chile (red), Southeast Asia (brown) and Tokyo (yellow).</p

Mutation rates of VNTRs of pandemic <i>V parahaemolyticus,</i> in relation to the number of repeats in each VNTR.

<p>White circles correspond to the mutation rates observed for any VNTR, circles in black correspond to the mutation rates observed for VNTR1 in the different clones. The best fit equation for VNTR1 was y = 0.1736×–1.1245 (R² = 0.9674) while that for all the VNTRs was y =  0.1598×– 0.7923 (R² = 0.8052).</p

Phylogenetic trees for KX-1 population obtained by MST-Manhattan (A) and MST categorical (B).

<p>Each circle corresponds to the different cluster of mutants. Numbers in the lines correspond to the differences in VNTRs repeat units between clusters.</p