7 research outputs found
Systematic Study in Mammalian Cells Showing No Adverse Response to Tetrahedral DNA Nanostructure
The
advent of DNA technology has demonstrated great potential in a wide
range of applications, especially in the field of biology and biomedicine.
However, current understanding of the toxicological effects and cellular
responses of DNA nanostructures remains to be improved. Here, we chose
tetrahedral DNA nanostructures (TDNs), a type of nanocarriers for
delivering molecular drugs, as a model for systematic live-cell analysis
of the biocompatibility of TDNs to normal bronchial epithelial cells,
carcinoma cells, and macrophage. We found that the interaction behaviors
of TDNs in different cell lines were very different, whereas after
internalization, most of the TDNs in diverse cell lines were positioned
to lysosomes. By a systematic assessment of cell responses after TDN
exposure to various cells, we demonstrate that internalized TDNs have
good innate biocompatibility. Interestingly, we found that TDN-bearing
cells would not affect the cell cycle progression and accompany cell
division and that TDNs were separated equally into two daughter cells.
This study improves our understanding of the interaction of DNA nanostructures
with living systems and their biocompatibility, which will be helpful
for further designing DNA nanostructures for biomedical applications
Systematic Study in Mammalian Cells Showing No Adverse Response to Tetrahedral DNA Nanostructure
The
advent of DNA technology has demonstrated great potential in a wide
range of applications, especially in the field of biology and biomedicine.
However, current understanding of the toxicological effects and cellular
responses of DNA nanostructures remains to be improved. Here, we chose
tetrahedral DNA nanostructures (TDNs), a type of nanocarriers for
delivering molecular drugs, as a model for systematic live-cell analysis
of the biocompatibility of TDNs to normal bronchial epithelial cells,
carcinoma cells, and macrophage. We found that the interaction behaviors
of TDNs in different cell lines were very different, whereas after
internalization, most of the TDNs in diverse cell lines were positioned
to lysosomes. By a systematic assessment of cell responses after TDN
exposure to various cells, we demonstrate that internalized TDNs have
good innate biocompatibility. Interestingly, we found that TDN-bearing
cells would not affect the cell cycle progression and accompany cell
division and that TDNs were separated equally into two daughter cells.
This study improves our understanding of the interaction of DNA nanostructures
with living systems and their biocompatibility, which will be helpful
for further designing DNA nanostructures for biomedical applications
Systematic Study in Mammalian Cells Showing No Adverse Response to Tetrahedral DNA Nanostructure
The
advent of DNA technology has demonstrated great potential in a wide
range of applications, especially in the field of biology and biomedicine.
However, current understanding of the toxicological effects and cellular
responses of DNA nanostructures remains to be improved. Here, we chose
tetrahedral DNA nanostructures (TDNs), a type of nanocarriers for
delivering molecular drugs, as a model for systematic live-cell analysis
of the biocompatibility of TDNs to normal bronchial epithelial cells,
carcinoma cells, and macrophage. We found that the interaction behaviors
of TDNs in different cell lines were very different, whereas after
internalization, most of the TDNs in diverse cell lines were positioned
to lysosomes. By a systematic assessment of cell responses after TDN
exposure to various cells, we demonstrate that internalized TDNs have
good innate biocompatibility. Interestingly, we found that TDN-bearing
cells would not affect the cell cycle progression and accompany cell
division and that TDNs were separated equally into two daughter cells.
This study improves our understanding of the interaction of DNA nanostructures
with living systems and their biocompatibility, which will be helpful
for further designing DNA nanostructures for biomedical applications
Systematic Study in Mammalian Cells Showing No Adverse Response to Tetrahedral DNA Nanostructure
The
advent of DNA technology has demonstrated great potential in a wide
range of applications, especially in the field of biology and biomedicine.
However, current understanding of the toxicological effects and cellular
responses of DNA nanostructures remains to be improved. Here, we chose
tetrahedral DNA nanostructures (TDNs), a type of nanocarriers for
delivering molecular drugs, as a model for systematic live-cell analysis
of the biocompatibility of TDNs to normal bronchial epithelial cells,
carcinoma cells, and macrophage. We found that the interaction behaviors
of TDNs in different cell lines were very different, whereas after
internalization, most of the TDNs in diverse cell lines were positioned
to lysosomes. By a systematic assessment of cell responses after TDN
exposure to various cells, we demonstrate that internalized TDNs have
good innate biocompatibility. Interestingly, we found that TDN-bearing
cells would not affect the cell cycle progression and accompany cell
division and that TDNs were separated equally into two daughter cells.
This study improves our understanding of the interaction of DNA nanostructures
with living systems and their biocompatibility, which will be helpful
for further designing DNA nanostructures for biomedical applications
Design, Synthesis, and Biological Evaluation of Potent and Selective PROTAC Degraders of Oncogenic KRAS<sup>G12D</sup>
KRASG12D, the most frequent KRAS oncogenic
mutation,
is a promising target for cancer therapy. Herein, we report the design,
synthesis, and biological evaluation of a series of KRASG12D PROTACs by connecting the analogues of MRTX1133 and the VHL ligand.
Structural modifications of the linker moiety and KRAS inhibitor part
suggested a critical role of membrane permeability in the degradation
activity of the KRASG12D PROTACs. Mechanism studies with
the representative compound 8o demonstrated that the
potent, rapid, and selective degradation of KRASG12D induced
by 8o was via a VHL- and proteasome-dependent manner.
This compound selectively and potently suppressed the growth of multiple
KRASG12D mutant cancer cells, displayed favorable pharmacokinetic
and pharmacodynamic properties in mice, and showed significant antitumor
efficacy in the AsPC-1 xenograft mouse model. Further optimization
of 8o appears to be promising for the development of
a new chemotherapy for KRASG12D-driven cancers as the complementary
therapeutic strategy to KRAS inhibition
Design, Synthesis, and Biological Evaluation of Potent and Selective PROTAC Degraders of Oncogenic KRAS<sup>G12D</sup>
KRASG12D, the most frequent KRAS oncogenic
mutation,
is a promising target for cancer therapy. Herein, we report the design,
synthesis, and biological evaluation of a series of KRASG12D PROTACs by connecting the analogues of MRTX1133 and the VHL ligand.
Structural modifications of the linker moiety and KRAS inhibitor part
suggested a critical role of membrane permeability in the degradation
activity of the KRASG12D PROTACs. Mechanism studies with
the representative compound 8o demonstrated that the
potent, rapid, and selective degradation of KRASG12D induced
by 8o was via a VHL- and proteasome-dependent manner.
This compound selectively and potently suppressed the growth of multiple
KRASG12D mutant cancer cells, displayed favorable pharmacokinetic
and pharmacodynamic properties in mice, and showed significant antitumor
efficacy in the AsPC-1 xenograft mouse model. Further optimization
of 8o appears to be promising for the development of
a new chemotherapy for KRASG12D-driven cancers as the complementary
therapeutic strategy to KRAS inhibition
Design, Synthesis, and Biological Evaluation of Potent and Selective PROTAC Degraders of Oncogenic KRAS<sup>G12D</sup>
KRASG12D, the most frequent KRAS oncogenic
mutation,
is a promising target for cancer therapy. Herein, we report the design,
synthesis, and biological evaluation of a series of KRASG12D PROTACs by connecting the analogues of MRTX1133 and the VHL ligand.
Structural modifications of the linker moiety and KRAS inhibitor part
suggested a critical role of membrane permeability in the degradation
activity of the KRASG12D PROTACs. Mechanism studies with
the representative compound 8o demonstrated that the
potent, rapid, and selective degradation of KRASG12D induced
by 8o was via a VHL- and proteasome-dependent manner.
This compound selectively and potently suppressed the growth of multiple
KRASG12D mutant cancer cells, displayed favorable pharmacokinetic
and pharmacodynamic properties in mice, and showed significant antitumor
efficacy in the AsPC-1 xenograft mouse model. Further optimization
of 8o appears to be promising for the development of
a new chemotherapy for KRASG12D-driven cancers as the complementary
therapeutic strategy to KRAS inhibition