Effect of LLL on mRNA expression and production and of IL-1β, IL4, IL-6, IL-8 and IL-10 by LPS (or not) induced MSCs.

<p>MSCs were incubated with or without LPS (10ng/mL) and simultaneous treated LLL for 1h. Values of *P<0.05, **P<0.01and vs. LPS or control were considered statistically significant.</p

LLL decrease nuclear translocation of NF-κB.

<p>The images show the cytoplasmic localization of NF-κB in the control cells (upper panel), the nuclear translocation of NF-κB in cells treated with LPS (middle panel) and LLL treatment blocked the nuclear translocation of NF-κB caused by LPS stimulation (lower panel).</p

Primers used in study for PCR.

<p>Primers used in study for PCR.</p

Percentage of positive PBMC after stimulation with PHA in the absence or presence of 3 types of treated MSCs.

<p>After reduction of LLL treated MSCs, CD25+ cells decreased (**P < 0.01 vs. PHA) and CD69+ cells were also reduced compared to control groups (**P < 0.01 vs. PHA).</p

LLL suppress ROS and NO promotion induced by LPS in MSCs.

<p>MSCs were incubated with LPS (10ng/mL) and simultaneous treated with LLL for 1 h. Error bars represent the mean±SD (n = 3 per group). Values of **P<0.01 vs. LPS and **P<0.01 vs. control were considered statistically significant.</p

LLL suppress the LPS-induced activation of the NF-κB pathway in MSCs.

<p>Protein samples were analyzed by Western blot using anti p65, p-p65, IκBα and p-IκBα antibody (left), GAPDH was used as the internal control for normalization. The bar chart shows the quantitative evaluation of protein bands by densitometry (right). The data represent the mean±SD (n = 3 per group) *P<0.05, **P<0.01 vs. LPS or control were considered statistically significant.</p

Additional file 1: of Lung tumor exosomes induce a pro-inflammatory phenotype in mesenchymal stem cells via NFÎşB-TLR signaling pathway

mRNA expression changes of IL-6, IL-8 and MCP-1 in MSCs stimulated with exosomes after knockdown of TLR2 by siRNA combinations for 24h. (TIF 236 kb