A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome highlights the challenges associated with draft genomes and gene prediction in plants

Most published genome sequences are drafts, and most are dominated by computational gene prediction. Draft genomes typically incorporate considerable sequence data that are not assigned to chromosomes, and predicted genes without quality confidence measures. The current Actinidia chinensis (kiwifruit) 'Hongyang' draft genome has 164\ua0Mb of sequences unassigned to pseudo-chromosomes, and omissions have been identified in the gene models

Aberrant processing of plasma vitronectin and high-molecular-weight kininogen precedes the onset of preeclampsia

Marion Blumenstein, Roneel Prakash, Garth J. S. Cooper, Robyn A. Nort

Additional file 5: Figure S4. of Cell separation in kiwifruit without development of a specialised detachment zone

Western analyses of total protein extracts from good-peeling and poor-peeling A. eriantha genotypes. Band patterns are highlighted with black bars in each panel. Peelability stages: ND, detachability not developed; DD detachability developing; FD, fully detachable peel. Immunoreactive bands in outer pericarp (OP) and peel are shown for β-Gal (A, B); EXP3 (C, D) and XTH7 (E, F). No bands were observed using the other antibodies. (JPEG 34 kb

Additional file 1: Figure S1. of Cell separation in kiwifruit without development of a specialised detachment zone

Toluidine blue O-stained sections of skin and hypodermal tissue from the fruit of the good-peeling (A) and poor-peeling (D) Actinidia eriantha genotypes. S, compressed skin layer consisting of dead cells; H1, narrow layer of thin walled hypodermal cells; H2, thicker walled more collenchyma-like hypodermal layer. Bar = 100 μm. (JPEG 1925 kb

Additional file 4: of A manually annotated Actinidia chinensis var. chinensis (kiwifruit) genome highlights the challenges associated with draft genomes and gene prediction in plants

BLASTP comparison of manually edited gene models to the revised ‘Hongyang’ gene models. List of best reciprocal BLASTp matches between the revised Actinidia chinensis ‘Hongyang’ genes [18]and the Red5 gene set (XLSX 436 kb