Pupal production and corresponding sex separation data at 24(SET), water temperature (T°C), larval diet (DIET) and larval densities (L/ML).

<p>Different letters within each column represent statistically significant differences between means with P<0.05 level (GLM with Tukey's post-hoc pairwise comparisons). Parentheses enclose standard errors of each mean value. EXP = Experiment number; N = Replicates number; NPP = pupae passed through the sieve for sex separation; %MPP = percentage of male in the pupae passed; NPP/NL₁ = pupal production; NPNP = pupae not passed through the sieve for sex separation; %MPNP = percentage of male in the pupae not passed.</p><p>*Data from each replicate were generated by processing pupae collected simultaneously from 10 trays stacked in the rack.</p

Mean escape rate of irradiated and unirradiated males from flight organs with tubes of 40 cm (left) and 80 cm (right) at 48 h, 72 h and 120 h from pupal introduction.

<p>Mean represents back-transformed data and bars indicate 95% CIs. Different letters represent statistically significant differences of means at p < 0.05 level (GLM with Tukey's HSD) between treatments at the same time or dose groups.</p

Survival curves of irradiated and unirradiated males.

<p>Crosses at day 5 and day 30 indicate censored data at the selected time for survival comparison between treatments. Different letters represent statistically significant differences of survival distributions at p < 0.05 level (Mantel-Cox log-rank test) for the different levels of radiation.</p

FAO/IAEA larval rearing unit (left) and standard laboratory trays used for <i>Aedes albopictus</i> larval mass rearing procedures (right).

<p>FAO/IAEA larval rearing unit (left) and standard laboratory trays used for <i>Aedes albopictus</i> larval mass rearing procedures (right).</p

Pupal production and larval survival rate registered within three days from the beginning of pupation (24, 48 and 72 hr) resulting from varying larval densities.

<p>Different letters represent statistically significant difference among means with P<0.05 level (GLM with Tukey's post-hoc pairwise comparisons). Parentheses enclose standard errors of each mean value. EXP = experiment number; SET = rearing method; T°C = water temperature; DIET = larval diet; L/ML = larval density; N = replicates number. NP = pupae produced; %M = percentage of males in the pupal collection; LARVAL SURVIVAL = larval survival rate at 72 h from pupation onset.</p

Mean escape rate of irradiated and unirradiated males from the aspirator device after 1h, 2h and 3h of aspiration.

<p>Means represent back-transformed data and bars indicate 95% CIs. Different letters represent statistically significant differences of means at p < 0.05 level (GLM with Tukey's HSD) between treatments at the same time or dose groups.</p

Mean male escape rate (left) and time (right) from flight organs with different tubes heights.

<p>Mean represents back-transformed data and bars indicate 95% CIs. Different letters represent statistically significant differences among means at p < 0.05 level (GLM with Tukey's HSD).</p

Use of the male escape rates from the flight organ and the aspirator devices to predict adult male quality parameters.

<p>Use of the male escape rates from the flight organ and the aspirator devices to predict adult male quality parameters.</p

Mean insemination rate (IR; left y-axis), full insemination rate (FIR; left y-axis) and overall number of spermathecae filled (NSF; right y-axis) by a single irradiated or unirradiated male caged with 10 females during a 5 day period.

<p>Means represents back-transformed data and bars indicate 95%CIS. Different letters represent statistically significant differences of means at p < 0.05 level (GLM with Tukey's HSD) between treatments per each parameter plotted.</p

Historical demographic analyses.

<p>(A) Mismatch distribution computed using the software Arlequin 3.1. Histograms show the observed distribution; lines show the expected distribution under a model of sudden population expansion. (B) Bayesian Skyline Plot, constructed using the software beast 1.6.1. Population size (<i>y</i>-axis) is measured as the product of effective population size per generation length (<i>N</i>_eτ). The solid line is the median estimate, and the grey areas show the 95% Highest Posterior Density (HPD) limits. Time (<b><i>x</i></b> axis) is expressed in years before present (BP).</p