Morphological changes in the rat aorta endothelium at the clamping sites

This study aimed to investigate on en face preparations the morphological changes in the rat aorta endothelium at the clamping sites while performing surgical anastomosis.Adult male Wistar rats (14-18-month-old, 390-420g) were used for the experiments. Under surgical anesthesia, the postrenal part of the abdominal aorta was dissected, clamped proximally and distally, cut and restored microsurgically using 10-0 suture. The clamping time was 30-40 min. At different days after surgery the animals were sacrificed, their aortas fixed and removed from the body and preparations for en face observation were made.En face preparations, obtained at the first postoperative day, showed complete denudation of the clamping sites. The most interesting endothelial cell population was noted at the borders of the clamping sites on the 3rd day after surgery - a large number of small endothelial cells and also few endothelial cells having very large size. At the later stages, the endothelial cell layer advanced rapidly to the denuded areas, as the complete restoration was observed after day 14

Morphological changes in the wall of great saphenous vein after radiofrequency ablation

Radiofrequency ablation (RFA) is a relatively new method for endoluminal thermal occlusion of the incompetent saphenous veins. The aim of the present study was to investigate microscopically the changes in the venous wall after routine RFA procedures. Short pieces (n=7) from the knee segment of the great saphenous vein were taken during RFA procedures. The removed vein segments were immersion fixed in 10% formalin and proceed to routine histology examination. Microscopically, the venous wall after RFA showed circular disintegration of the intimal layer. In addition, cylindrical medial lesions with disintegration and intercellular splits and gaps were observed. No transmural thermal lesions were seen. The present results highlight the mechanism of predetermined tissue damage after RFA procedures of the great saphenous vein

CONTRALATERAL PROPHYLACTIC MASTECTOMY AND SIMULTANEOUS BREAST RECONSTRUCTION WITH IMPLANTS AFTER ONE SIDED BREAST CANCER: ANALYSIS OF 12 CASES IN CONTEXT OF THE CURRENT TRENDS

The contemporary tendency for increase of prophylactic surgical procedures as part of the sophisticated treatment of breast cancer is the foundation for the present study. Evaluation of risk factors for developing contralateral breast cancer, factors for an increase in prophylactic mastectomies and indications for performing those operations are briefly commented. Analysis of 12 cases of the authors surgical practice is presented regarding treatment approach from the oncological, reconstructive and aesthetic point of view. Results, complications, alternative methods, clinical importance and possible future aspects are discussed