Nachrichten aus der Brüder-Gemeine, 1828, no. 01

The Moravian Church's monthly journal of its worldwide activities, including in Labrador, published from 1819-94

Bondholder Coercion: The Problem of Constrained Choice in Debt Tender Offers and Recapitalizations

<p>The effect MATE1, MATE2 and PMAT inhibitors on the [³H]pentamidine accumulation in hCMEC/D3 (A) and bEnd.3 (B) cells. Cells were incubated with MATE1 inhibitor famotidine (1 μM), MATE2 inhibitor nifekalant (3 μM) or PMAT inhibitor lopinavir (2 μM) and no significant differences were observed compared to control. All data expressed as mean ± SEM, n =.4 passages of cells, with 6 replicates (wells) per timepoint per plate. Data were analysed using two-way ANOVA with SigmaPlot 13.0.</p

Organic cation transporter 1 (OCT1) is involved in pentamidine transport at the human and mouse blood-brain barrier (BBB) - Fig 10

<p>Confocal microscopy image of MRP4 expression in confluent hCMEC/D3 cells (A) and bEnd.3 cells (B). No fluorescent signals were detected when using MRP4 antibody [M4I-10] from Abcam at 1:200 dilution and goat anti-rat Alexa Fluor^® 488 secondary antibody (Abcam, ab181448) at 1:200 dilution.</p

The transporter inhibitors used in this study along with [³H]pentamidine.

<p>All inhibitors were used in the presence of 0.05% DMSO and used at the published concentration ranges where they affect transporter activity.</p

BCRP expression in confluent hCMEC/D3 cells and bEnd.3 cells.

<p>Confocal microscopy image of BCRP expression in confluent hCMEC/D3 cells (A) and bEnd.3 cells (B) was not detected in the plasma membranes when using anti-BCRP antibody (New England Biolabs, 4477S diluted at 1:200)—Western blot band of BCRP was detected at 143 kD which corresponds to the dimer version of BCRP in positive control (C). No band was observed at 70 kD (monomer version of BCRP). Tubulin (55 kD) was used as a loading control. Lane 1 –hCMEC/D3 passage 28, Lane 2- hCMEC/D3 passage 33, Lane 3- bEnd.3 passage 18, Lane 4- bEnd.3 passage 23, Lane 5 –brain endothelial cells isolated from mouse (positive control).</p

Expression of OCT1 was detected by Transmission Electron Microscopy and immunogold labelling.

<p>hCMEC/D3 (p28) and bEnd.3 cells (p23) were grown to confluency on transwell polycarbonate membrane and stained for OCT1 using the method described. Gold nanoparticles on the membranes (arrows) were counted in the 60 sequential images acquired at 11500 x magnification. Significantly increased expression of OCT1 was found on the luminal membrane compared to the abluminal membrane. ***p<0.001 using student’s t-test.</p

Binding of C3 (A and C) and MAC (B and D) to bacteria detected by FACS analysis after incubation in immune human serum pooled from 10 donors

Results are shown as the MFI (calculated as geometric mean multiplied by the percentage of gated cells), and the error bars show the SEM. In B and D, strains were incubated with serum in the presence of magnesium and EGTA to block the CP and LP (-CP/LP). Error bars show the SEM of assays performed in triplicate. There was significantly less binding of complement factors to R3 and S3∷R compared with S3. P < 0.01 in all assays with the Student's test.<p><b>Copyright information:</b></p><p>Taken from "A generic mechanism in for enhanced resistance against bactericidal antibodies"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1423-1434.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413038.</p><p></p

Capsular material was detected around the surface of the sensitive strain S3 (A and B, arrows) but not the corresponding mutant (C and D)

Strains R3 and S3∷R (E and F; and G and H, respectively) expressed larger amounts of capsule, which formed large extracellular aggregates in places. Bars, 400 nm.<p><b>Copyright information:</b></p><p>Taken from "A generic mechanism in for enhanced resistance against bactericidal antibodies"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1423-1434.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413038.</p><p></p

No significant difference was identified between the capsules from strains S3 (A) and R3 (B)

The asterisk denotes the internal standard (acetone), and the prominent and distorted peaks at ∼4.8 parts per million (ppm) in both spectra are caused by partially deuterated water.<p><b>Copyright information:</b></p><p>Taken from "A generic mechanism in for enhanced resistance against bactericidal antibodies"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1423-1434.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413038.</p><p></p

The relative amounts of (A) and (B) transcripts from S3, R3, and S3∷R measured by qrtRT-PCR

Error bars show the SEM of assays performed in triplicate. P < 0.005 for differences in and mRNA levels in R3 and S3∷R versus S3 using the Student's test. (C) Western blot analysis of SiaA and RecA in whole-cell lysates. The strains are indicated above each lane, and the sizes of the proteins (in kD) are shown. (D) Expression of the polysialic capsule detected by FACS. Bacteria were incubated with polyclonal antisera raised against the MCC vaccine (black, capsule-negative strain R3Δ; blue, S3; red, S3∷R; purple, R3). (E) Relative amount of capsule detected by FACS. Results are shown as the MFI (calculated as the geometric mean multiplied by the percentage of positive cells). Error bars show the SEM. P < 0.005 for both strains with increased resistance versus S3 using the Student's test.<p><b>Copyright information:</b></p><p>Taken from "A generic mechanism in for enhanced resistance against bactericidal antibodies"</p><p></p><p>The Journal of Experimental Medicine 2008;205(6):1423-1434.</p><p>Published online 9 Jun 2008</p><p>PMCID:PMC2413038.</p><p></p