Fraksi Etanol Daun Sembung Rambat (Mikania Micrantha Kunth) sebagai Antiinflamasi terhadap Tikus Putih Jantan Galur Wistar

Tanaman sembung rambat (Mikania micrantha Kunth) merupakan tanaman yang berasal dari family Anacardiaceae. Tanaman sembung rambat diketahui mengandung senyawa metabolit sekunder seperti alkaloid, flavonoid, saponin dan tannin. Flavonoid merupakan senyawa polifenol yang berfungsi sebagai antiinflamasi. Daun sembung rambat banyak mengandung flavonoid yang dapat berkhasiat sebagai antiinflamasi, antibakteri. Tujuan dari penelitian ini adalah untuk melihat pengaruh fraksi etanol daun sembung rambat (Mikania micrantha Kunth) berpengaruh terhadap antiinflamasi terhadap tikus putih jantan galur wistar. Pada penelitian ini daun sembung rambat diekstraksi dengan cara maserasi menggunakan etanol 70%. Setelah didapatkan ekstrak selanjutnya dilakukan prosen fraksinasi. Dari fraksi yang didapat kemudian dibuat 3 varian dosis yaitu 112.5 mg/kgBB, 225 mg/kgBB dan 450 mg/kbBB. Dari 3 dosis tersebut dosis 450 mg/kgBB memiliki pengaruh yang lebih bagus daripada yang lain. Kontrol positif menggunakan Asetosal dan kontrol negatif menggunakan larutan CMC 1%. Hasil penelitian ini menunjukkan bahwa fraksi etanol daun sembung rambat dapat menghambat pembengkakan terhadap kaki tikus putih jantan galur wistar. Makin besar konsentrasi fraksi, maka semakin besar penghambatan bengkak pada kaki tikus. Dosis 450 mg/kbBB memiliki nilai rata-rata AUC - 8,22 mm.menit. Hasil analisis menunjukkan fraksi etanol daaun sembung rambat berpengaruh sebagai antiinflamasi terhadap tikus putih jantan galur wistar. Kata kunci : Mikania micrantha Kunth. Jangka Sorong, Antiinflamasi, Flavonoi

Analisis Kadar Nitrit pada Sosis Sapi Kemasan yang Beredar di Pasar Tradisional Kota Bandar Lampung secara Spektrofotometri Uv-vis

The use of nitrite as a preservative and provider of the stable red color, aroma as well as taste for the meat is allowed within processed meat products, such as beef sausage. However, it should be noted that its use does not exceed the maximum permitted limit so as not to negatively impact human health. Excess nitrite in the human body can cause acute poisoning, namely methemoglobinemia and chronic poisoning as a result of the formation of carcinogenic nitrosamine derivatives. This research aims to determine whether the levels of nitrite contained in the packaged beef sausage distributed in the traditional market of Bandar Lampung is in accordance with the regulations of The Indonesia Food and Drug Authority or Indonesia FDA No. 36 of 2013 which is not more than 30 mg/kg. The analysis of nitrite levels in the sample was carried out using with UV-Vis Spectrophotometry method based on diazotation reaction between nitric acid and sulfanilamide forming diazonium salts. The products were then reacted again with naphthyl ethylenediamine to form a purplish red compound whose absorbance was measured at a wavelength 0f 543,8 nm. From the results of the analytical methods validation, it was obtained that the value of the correlation coefficient was 0,9997, the coefficient of determination 0,9994, the coefficient of variation 1,2121%, the range of percent recovery between 98,630-101,240%, and the detection and the quantitation limit respectively at 0,0187 ppm and 0,0623 ppm. This indicates that all test parameters meet the requirements for their use. The result of determining the average levels of nitrite in the samples show that there were two samples that had levels exceeding the maximum allowed limit, i.e sampel C=40,407 mg/kg and sample D=33,181mg/kg while sample A=18,963 mg/kg, sample B=9,427 mg/kg, sample E=9,423 mg/kg and sample F=8,689 mg/kg did not exceed the maximum allowed limit. Keywords: Beef Sausage, Nitrite, UV-Vis Spectrophotometry, Method AnalysisValidatio

Evaluasi Penggunaan Obat Pada Pasien Hipertensi Rawat Jalan Di Rumah Sakit Umum Wisma Rini Pringsewu Tahun 2019

Hypertension is a non-communicable disease characterized by the increase of systolic blood pressure is >140 mmHg and diastolic blood pressure is >90 mmHg. Hypertension is also the major risk factor of other cardiovascular disease. The incidence rate of hypertension with or without complications increases every year, so the potential for inaccurate use of drugs is increasing. The purpose of this research is to determine the characteristics of hypertension outpatients and the proper hypertension drug use in hypertension outpatients at Wisma Rini General Hospital Pringsewu in 2019 based on the proper patient, the proper indication, the proper drug, and the proper dose using the standard reference of Guidelines JNC VIII. This research is descriptive research, the data was collected retrospectively based on medical records. The sample collection technique in this research is using a purposive sampling technique. The results in this study obtained from 110 patient samples diagnosed with the hypertension showed that most of hypertension patients are women (55,46%), mostly aged 56-65 years old (40%), and the most taken hypertension drug is CCB group amlodipine with 78 patients (71%). The evaluation of antihypertensive drug use obtained is proper patient with 110 patients (100%), proper indication with 110 patients (100%), proper drug with 92 patients (75,6%), and proper dose with 110 patients (100%). Keywords: Antihypertensive, Hypertension, Accuracy drug use

Fraksi Air Ekstrak Daun Wungu (Graptopphyllumpictum L.) Sebagai Antiinflamasi Terhadap Tikus Putih Jantan

This study aims to determine the anti-inflammatory effect of the water extract of Daun wungu (Graptophyllum pictum L.) extract which was observed from the decrease in the volume of edema of rat's feet which was induced by 1 % carrageenan as much as 0,1 ml, was carried out in 25 male white rats divided into 5 groups. Negative controlwas given distilled water given 5 % Na CMC, diclofenac sodium as a positive control with a dose of 0,9 mg/200 g BB and water fraction of wungu leaf extract with a dose 1 (1,125 mg/kg bb), dose 2 (2,25 mg/kg bb) dan dose 3 (4,5 mg/kg bb). The measurement of edema volume is carried out every hour for six hours after induced carrageenan. From the results of testing the water fraction of wungu leaf extract, % inhibition showed that the dose group 3 (4,5 mg/kg bb) had anti-inflammatory activity that was comparable to the positive control group. Of all dosage variatons in this study the effective dose which has the highest percentage of edema is the dose 3 (4,5 mg/kg bb). Based on the results of statistical analysis, the percentage of edema inhibition data on the water fraction of wungu lear extract from all dose variations showed significant differences with negative controls. Keyword : Graptophyllum pictum L Anti-Inflamas