Short-term effects of CO₂-induced low pH exposure on target gene expression in Platynereis dumerilii

Objective: Increasing atmospheric CO₂ concentration are causing changes to the seawater carbonate chemistry, lowering the pH and we study potential impacts of these changes at the molecular level in a non-calcifying, marine polychaete species Platynereis dumerilii. Methods: we investigate the relative expression of carbonic anhydrase (CA), Na+/H+ exchangers (NHE), and calmodulin (CaM) genes from P. dumerilii under acidified seawater conditions (pH 7.8) induced by CO₂ using qPCR. Results: mRNA expression of CA in the CO₂-induced worms was significantly up-regulated at low pH conditions (pH 7.8, 1h), suggesting changes in acid-base balance. In contrast, the expression of NHE and CaM showed no significant change. In addition, we compare these results to a previous study using inorganic acid (HCl)-induced pH changes. Conclusions: results suggest that carbonate chemistry has an impact on gene expression that differs from pH-associated change. To our knowledge, this is the first study that compares low pH exposure experiments using HCl and CO₂ as the inducing agents

A steerable UV laser system for the calibration of liquid argon time projection chambers

A number of liquid argon time projection chambers (LAr TPC's) are being build or are proposed for neutrino experiments on long- and short baseline beams. For these detectors a distortion in the drift field due to geometrical or physics reasons can affect the reconstruction of the events. Depending on the TPC geometry and electric drift field intensity this distortion could be of the same magnitude as the drift field itself. Recently, we presented a method to calibrate the drift field and correct for these possible distortions. While straight cosmic ray muon tracks could be used for calibration, multiple coulomb scattering and momentum uncertainties allow only a limited resolution. A UV laser instead can create straight ionization tracks in liquid argon, and allows one to map the drift field along different paths in the TPC inner volume. Here we present a UV laser feed-through design with a steerable UV mirror immersed in liquid argon that can point the laser beam at many locations through the TPC. The straight ionization paths are sensitive to drift field distortions, a fit of these distortion to the linear optical path allows to extract the drift field, by using these laser tracks along the whole TPC volume one can obtain a 3D drift field map. The UV laser feed-through assembly is a prototype of the system that will be used for the MicroBooNE experiment at the Fermi National Accelerator Laboratory (FNAL)

Measurement of the drift field in the ARGONTUBE LAr TPC with 266~nm pulsed laser beams

ARGONTUBE is a liquid argon time projection chamber (LAr TPC) with a drift field generated in-situ by a Greinacher voltage multiplier circuit. We present results on the measurement of the drift-field distribution inside ARGONTUBE using straight ionization tracks generated by an intense UV laser beam. Our analysis is based on a simplified model of the charging of a multi-stage Greinacher circuit to describe the voltages on the field cage rings

Ion and polymer dynamics in polymer electrolytes PPO-LiClO4: II. 2H and 7Li NMR stimulated-echo experiment

We use 2H NMR stimulated-echo spectroscopy to measure two-time correlation functions characterizing the polymer segmental motion in polymer electrolytes PPO-LiClO4 near the glass transition temperature Tg. To investigate effects of the salt on the polymer dynamics, we compare results for different ether oxygen to lithium ratios, namely, 6:1, 15:1, 30:1 and infinity. For all compositions, we find nonexponential correlation functions, which can be described by a Kohlrausch function. The mean correlation times show quantitatively that an increase of the salt concentration results in a strong slowing down of the segmental motion. Consistently, for the high 6:1 salt concentration, a high apparent activation energy E_a=4.1eV characterizes the temperature dependence of the mean correlation times at Tg < T< 1.1T_g, while smaller values E_a=2.5eV are observed for moderate salt contents. The correlation functions are most nonexponential for 15:1 PPO-LiClO4, whereas the stretching is reduced for higher and lower salt concentrations. A similar dependence of the correlation functions on the evolution time in the presence and in the absence of ions indicates that addition of salt hardly affects the reorientational mechanism. For all compositions, mean jump angles of about 15 degree characterize the segmental reorientation. In addition, comparison of results from 2H and 7Li NMR stimulated-echo experiments suggests a coupling of ion and polymer dynamics in 15:1 PPO-LiClO4.Comment: 14 pages, 12 figure

Decay dynamics of quantum dots influenced by the local density of optical states of two-dimensional photonic crystal membranes

We have performed time-resolved spectroscopy on InAs quantum dot ensembles in photonic crystal membranes. The influence of the photonic crystal is investigated by varying the lattice constant systematically. We observe a strong slow down of the quantum dots' spontaneous emission rates as the two-dimensional bandgap is tuned through their emission frequencies. The measured band edges are in full agreement with theoretical predictions. We characterize the multi-exponential decay curves by their mean decay time and find enhancement of the spontaneous emission at the bandgap edges and strong inhibition inside the bandgap in good agreement with local density of states calculations.Comment: 9 pages (preprint), 3 figure

A method to suppress dielectric breakdowns in liquid argon ionization detectors for cathode to ground distances of several millimeters

We present a method to reach electric field intensity as high as 400 kV/cm in liquid argon for cathode-ground distances of several millimeters. This can be achieved by suppressing field emission from the cathode, overcoming limitations that we reported earlier

On the Electric Breakdown in Liquid Argon at Centimeter Scale

We present a study on the dependence of electric breakdown discharge properties on electrode geometry and the breakdown field in liquid argon near its boiling point. The measurements were performed with a spherical cathode and a planar anode at distances ranging from 0.1 mm to 10.0 mm. A detailed study of the time evolution of the breakdown volt-ampere characteristics was performed for the first time. It revealed a slow streamer development phase in the discharge. The results of a spectroscopic study of the visible light emission of the breakdowns complement the measurements. The light emission from the initial phase of the discharge is attributed to electro-luminescence of liquid argon following a current of drifting electrons. These results contribute to set benchmarks for breakdown-safe design of ionization detectors, such as Liquid Argon Time Projection Chambers (LAr TPC).Comment: Minor revision according to editor report. 17 pages, 15 figures, 2 tables. Turboencabulato