1 research outputs found
Surface Preparation Strategies for Improved Parallelization and Reproducible MALDI-TOF MS Ligand Binding Assays
Immunoassays are employed in academia and the healthcare
and biotech
industries for high-throughput, quantitative screens of biomolecules.
We have developed monolayer-based immunoassays for MALDI-TOF MS. To
improve parallelization, we adapted the workflow to photolithography-generated
arrays. Our work shows Parylene-C coatings provide excellent “solvent
pinning” for reagents and biofluids, enabling sensitive MS
detection of immobilized components. With a unique MALDI-matrix crystallization
technique we show routine interassay RSD <10% at picomolar concentrations
and highlight platform compatibility for relative and label-free quantitation
applications. Parylene-arrays provide high sample densities and promise
screening throughputs exceeding 1000 samples/h with modern liquid-handlers
and MALDI-TOF systems