Is Sugar Sweeter at the Pump? The Macroeconomic Impact of Brazil's Alternative Energy Program

The recent world energy crisis raises serious questions about the extent to which the United States should increase domestic oil production and develop alternative sources of energy. We examine the energy developments in Brazil as an important experiment. Brazil has reduced its share of imported oil more than any other major economy in the world in the last 30 years, from 70 percent in the 1970s to only 10 percent today. Brazil has largely achieved this goal by: (1) increasing domestic oil production and (2) developing one of the world's largest and most competitive sources of renewable energy -- sugarcane ethanol -- that now accounts for 50 percent of Brazil's total gasoline consumption. A counterfactual analysis of economic growth in Brazil from 1980-2008 suggests that GDP is almost 35 percent higher today because of increased domestic oil production and the development of sugarcane ethanol. We also find a notable reduction in business-cycle volatility as a result of Brazil's progression to a more diversified energy program. Nearly three-fourths of the welfare benefits have come from domestic oil drilling, however, as rents have been paid to domestic factors of production during a time of rising oil prices. We discuss the potential implications of Brazil's energy program for the U.S. economy by conducting historical counterfactual exercises on U.S. real GDP growth since the 1970s.

Environmental control of amnesic effects of various agents in goldfish

Fish given a single electroconvulsive shock or intracranial administration of puromycin, acetoxycycloheximide, or KCl immediately following shock avoidance training show amnesia on retraining days later. When the treatments are given 24 hr after training no amnesia develops. But amnesia can be obtained 24 hr after training with KCl, puromycin and acetoxycycloheximide, if the fish are replaced in the intertrial environment for a brief period just prior to injection. Implications of these results to the consolidation hypothesis of memory formation are discussed. Previously unreported results on the amnesic effects of intracranial KCl in goldfish are also described.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/33008/1/0000392.pd

Spatial discrimination in goldfish following bilateral tectal ablation

Goldfish were classically conditioned to discriminate between right and left or nasal and temporal presentations of a spot-of-light conditioned stimulus (CS). After the conditioning, the fish were administered bilateral optic tectum ablation followed by weekly sessions of conditioning trials to test for retention or relearning of the discrimination response. As their behavioral photosensitivity is greatly decreased, the ablates were dark-adapted prior to each session and trials were administered in darkness. Right x left discrimination was retained postoperatively but the nasal x temporal discrimination was blocked. Sham-operated controls discriminated between the nasal and temporal CS when dark-adapted and tested in darkness. Subsequent transection of the optic nerves obliterated response to the CS, indicating that tectum ablates detect and respond to the CS retinally and not extraretinally. We conclude that memory of visual spatial learning is mediated by non-tectal brain structures and that the ablate can discriminate between right- and left-eye input but sees the CS too diffusely to distinguish its location within the monocular field.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/26590/1/0000131.pd

Nervus terminalis innervation of the goldfish retina and behavioral visual sensitivity

The possibility that axon terminals of the nervus terminalis in the goldfish retina regulate visual sensitivity was examined psychophysically. Fish were classically conditioned to respond in darkness to a diffuse red light conditioned stimulus. Bilateral ablation of the olfactory bulb and telencephalon had no significant effect on response threshold which was measured by a staircase method. Retinopetal nervus terminalis fibres thus appear to play no role in maintaining scotopic photosensitivity.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/27173/1/0000170.pd

Stabilising Lyme Regis – a strategic approach

Coastal erosion and landslides have been a constant threat to Lyme Regis in West Dorset, UK for over 250 years. By the 1980s, the frequency and scale of coastal erosion and land instability had reached a point whereby the local council realised that a change from the previous ad hoc repair and protection approach was needed to secure the long-term future of the town. An environmental improvements initiative was developed from then onwards to provide a strategic and integrated programme of coast protection and cliff stabilisation measures designed to mitigate the increasing threat of climate change, coastal erosion and landslides, while respecting the site’s unique heritage and environmental interests. This paper outlines the background and principal phases of the project that have been successfully delivered over the period 1990–2015

Autoradiographic localization of sex steroid-concentrating cells in the brain of the teleost Macropodus opercularis (Osteichthyes: Belontiidae)

Tritiated estradiol or testosterone was administered to gonadectomized male paradise fish, Macropodus opercularis, to investigate the neuroanatomical location of sex steroid-retaining cells. Each male was sacrificed 2 hr following intraperitoneal injection of the labeled hormone. Autoradiograms were prepared, and sections were taken from the entire brain, the anterior spinal cord, and the pituitary. Following 4 to 12 months of exposure, the distribution of labeled cells was seen to be the same for males which had received the estradiol as those which received testosterone, but estradiol resulted in a greater number of labeled cells. Steroid-concentrating cells were located in the ventral telencephalon, preoptic area, lateral tuberal nucleus, nucleus of the lateral recess of the third ventricle, and caudal portion of the posterior periventricular nucleus. In addition, the caudal pars distalis of the pituitary contained many labeled cells. No steroid-retaining cells were seen in the mesencephalon, rhombencephalon, or anterior spinal cord.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/22799/1/0000355.pd

Mitogen Kinase Kinase (MKK7) Controls Cytokine Production In Vitro and In Vivo in Mice

Mitogen kinase kinase 4 (MKK4) and mitogen kinase kinase 7 (MKK7) are members of the MAP2K family that can activate downstream mitogen-activated protein kinases (MAPKs). MKK4 has been implicated in the activation of both c-Jun N-terminal kinase (JNK) and p38 MAPK, while MKK7 has been reported to activate only JNK in response to different stimuli. The stimuli, as well as the cell type determine which MAP2K member will mediate a given response. In various cell types, MKK7 contributes to the activation of downstream MAPKs, JNK, which is known to regulate essential cellular processes, such as cell death, differentiation, stress response, and cytokine secretion. Previous studies have also implicated the role of MKK7 in stress signaling pathways and cytokine production. However, little is known about the degree to which MKK4 and MKK7 contribute to innate immune responses in macrophages or during inflammation in vivo. To address this question and to elucidate the role of MKK4 and MKK7 in macrophage and in vivo, we developed MKK4- and MKK7-deficient mouse models with tamoxifen-inducible Rosa26 Cre(ERT). This study reports that MKK7 is required for JNK activation both in vitro and in vivo. Additionally, we demonstrated that MKK7 in macrophages is necessary for lipopolysaccharide (LPS)-induced cytokine production, M1 polarization, and migration, which appear to be a major contributor to the inflammatory response in vivo. Conversely, MKK4 plays a significant, but minor role in cytokine production in vivo

The p38 MAPK pathway is essential for skeletogenesis and bone homeostasis in mice

Nearly every extracellular ligand that has been found to play a role in regulating bone biology acts, at least in part, through MAPK pathways. Nevertheless, much remains to be learned about the contribution of MAPKs to osteoblast biology in vivo. Here we report that the p38 MAPK pathway is required for normal skeletogenesis in mice, as mice with deletion of any of the MAPK pathway member–encoding genes MAPK kinase 3 (Mkk3), Mkk6, p38a, or p38b displayed profoundly reduced bone mass secondary to defective osteoblast differentiation. Among the MAPK kinase kinase (MAP3K) family, we identified TGF-β–activated kinase 1 (TAK1; also known as MAP3K7) as the critical activator upstream of p38 in osteoblasts. Osteoblast-specific deletion of Tak1 resulted in clavicular hypoplasia and delayed fontanelle fusion, a phenotype similar to the cleidocranial dysplasia observed in humans haploinsufficient for the transcription factor runt-related transcription factor 2 (Runx2). Mechanistic analysis revealed that the TAK1–MKK3/6–p38 MAPK axis phosphorylated Runx2, promoting its association with the coactivator CREB-binding protein (CBP), which was required to regulate osteoblast genetic programs. These findings reveal an in vivo function for p38β and establish that MAPK signaling is essential for bone formation in vivo. These results also suggest that selective p38β agonists may represent attractive therapeutic agents to prevent bone loss associated with osteoporosis and aging