Final multivariate model of the risk of developing open-angle glaucoma for cholesterol-lowering medication.

<p>NSCLDs = non-statin cholesterol-lowering drugs; IOP = intraocular pressure.</p

Univariable analyses of the use of cholesterol-lowering medication at any time during follow-up and the development of open-angle glaucoma.

<p>NSCLDs = non-statin cholesterol-lowering drugs.</p

Baseline characteristics of participants with and without incident open-angle glaucoma (A) and of cholesterol-lowering drug users (either statins or NSCLDs, or both) and non-users (B), with univariable comparisons (mean values with standard deviation between brackets unless stated otherwise).

<p>NSCLDs = non-statin cholesterol-lowering drugs; IOP = intraocular pressure.</p

Multiple linear regression analysis with intraocular pressure at follow-up as the dependent variable.

<p>IOP = intraocular pressure.</p

Association of rs11656696 and rs7555523 with glaucoma.

<p>Association of rs11656696 and rs7555523 with glaucoma.</p

Expression levels of GAS7 and TMCO1 in human ocular tissues.

<p>Quantitative determination of <i>GAS7</i> and <i>TMCO1</i> mRNA expression levels in human ocular tissues by using real-time PCR technology (n = 4). The expression levels were normalized against GAPDH and the results are expressed as copynumber/µg RNA. Co, cornea; Tr, trabecular meshwork; Ir, iris; Le, lens; Ci, ciliary body; Re, retina; Ch, choroid; La, lamina cribrosa; Op, optic nerve.</p

Characteristics of the discovery cohorts.

<p>IOP = intraocular pressure; SD = standard deviation; RS = Rotterdam Study; ERF = Erasmus Rucphen Family study.</p

Gene expression levels in human ocular tissues.

<p>The two genes are ranked by increasing expression, calculated by the mean percentiles (SD) of the expression levels. Gene expression of CB-PE and CB-NPE (n = 4), choroid (n = 3), photoreceptors (n = 3) and RPE (n = 6) were performed on Agilent Human 44k microarray of post-mortem donor eyes without glaucoma or any other ocular diseases.</p>*<p>Data from Liton et al., performed on Affymetrix Human U133 microarray, showing mean percentiles (SD) of human gene expression levels in TM tissue from 3 healthy eyes <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002611#pgen.1002611-Liton1" target="_blank">[28]</a>.</p><p>CB-PE = ciliary body, pigmented epithelium; CB-NPE = ciliary body, non-pigmented epithelium; RPE = retinal pigment epithelium; TM = trabecular meshwork.</p

Regional association plots of the 17p13.1 and 1q24.1 regions in the discovery meta-analysis.

<p>Regional association plots of the 17p13.1 and 1q24.1 regions in the discovery meta-analysis.</p

Results of the meta-analysis of the gene discovery cohorts: loci associated with IOP (p<10⁻⁵).

<p>SNP = single nucleotide polymorphism; Chrom = chromosome; MA(F) = minor allele (frequency); SE = standard error.</p>*<p>number of SNPs with p<10⁻⁵ in the region.</p><p>According NCBI build 37.1, rs11656696 is located at position 10033679 in the growth-arrest-specific gene <i>GAS7</i> while an earlier build allocated the SNP at 9974404 (<a href="http://www.ncbi.nlm.nih.gov" target="_blank">http://www.ncbi.nlm.nih.gov</a>).</p