Is HIV-1 RNA dimerization a prerequisite for packaging? Yes, no, probably?

During virus assembly, all retroviruses specifically encapsidate two copies of full-length viral genomic RNA in the form of a non-covalently linked RNA dimer. The absolute conservation of this unique genome structure within the Retroviridae family is strong evidence that a dimerized genome is of critical importance to the viral life cycle. An obvious hypothesis is that retroviruses have evolved to preferentially package two copies of genomic RNA, and that dimerization ensures the proper packaging specificity for such a genome. However, this implies that dimerization must be a prerequisite for genome encapsidation, a notion that has been debated for many years. In this article, we review retroviral RNA dimerization and packaging, highlighting the research that has attempted to dissect the intricate relationship between these two processes in the context of HIV-1, and discuss the therapeutic potential of these putative antiretroviral targets

Evaluating lightning hazards to building environments using explicit numerical solutions of Maxwell's equations

The objective here is to describe the lightning hazards to buildings and their internal environments using advanced formulations of Maxwell's Equations. The method described is the Three Dimensional Finite Difference Time Domain Solution. It can be used to solve for the lightning interaction with such structures in three dimensions with the inclusion of a considerable amount of detail. Special techniques were developed for including wire, plumbing, and rebar into the model. Some buildings have provisions for lightning protection in the form of air terminals connected to a ground counterpoise system. It is shown that fields and currents within these structures can be significantly high during a lightning strike. Time lapse video presentations were made showing the electric and magnetic field distributions on selected cross sections of the buildings during a simulated lightning strike

A Discontinuity in the Distribution of Fixed Point Sums

The quantity $f(n,r)$, defined as the number of permutations of the set $[n]=\{1,2,... n\}$ whose fixed points sum to $r$, shows a sharp discontinuity in the neighborhood of $r=n$. We explain this discontinuity and study the possible existence of other discontinuities in $f(n,r)$ for permutations. We generalize our results to other families of structures that exhibit the same kind of discontinuities, by studying $f(n,r)$ when ``fixed points'' is replaced by ``components of size 1'' in a suitable graph of the structure. Among the objects considered are permutations, all functions and set partitions.Comment: 1 figur

Conditional-moment Closure with Differential Diffusion for Soot Evolution in Fire

The conditional-moment closure (CMC) equation for the evolution of a large Lewis number scalar, soot, is derived starting from the joint probability density function (pdf) equation for the gas-phase mixture fraction, ξ g , and the soot mass fraction, Y s . Unlike previous approaches starting with the joint pdf, the residual terms that result from the typical closure models were retained. A new formulation of the one-dimensional turbulence (ODT) model suitable for spatially evolving flows with buoyant acceleration and radiative transport in participating media was employed to carry out simulations of a prototypical ethene fire. The resulting ODT evolution of ξ g and Y s was used to assess the significance of various terms in the CMC equation including the residual correlations. The terms involving differential diffusion are found to be important along with the soot source terms and the large-scale evolution of both ξ g and Y s . Of particular importance in the regions in mixture-fraction space around the soot production and consumption is a residual term, not previously identified, related to the correlation between the differential diffusion and Y s . This term results in a diffusion-like behavior of Y s in the mixture fraction coordinate that has an apparent Lewis number near unity. In scenarios where the large Lewis number component is a non-negligible component of the mixture fraction (i.e., large soot loading), it is found easier to employ a mixture fraction neglecting this component. Such a mixture-fraction variable has a chemical source term, but this appears easier to model than the differential diffusion and dissipation terms that result when the large Lewis number component is retained in the mixture-fraction definition

Stereospecific four-bond phosphorus-phosphorus spin couplings in phosphazenyl-phosphazenes

Four-bond phosphorus-phosphorus coupling constants have been measured from the 31P NMR spectra of phosphazenylcyclophosphazenes. Their magnitude appears to be related to the conformation adopted by the phosphazenyl-group relative to the phosphazene ring

FEASIBILITY OF AN OKLAHOMA FRESH GREENS AND COWPEAS PACKING COOPERATIVE

Oklahoma's green producers are not benefiting from a growing fresh market. In order to seize the opportunities offered by the growing fresh market for leafy greens, investment in packing facilities have been evaluated. To make use of these facilities during summer months, the addition of a cowpea shelling enterprise is considered. A business plan for a new generation cooperative is estimated using an updated version of "The Packing Simulation Model" (PACKSIM) The business associates PACKSIM with @RISK®, to incorporate risks in the financial analysis.Agribusiness,

Low computation vision-based navigation for a Martian rover

Construction and design details of the Mobot Vision System, a small, self-contained, mobile vision system, are presented. This system uses the view from the top of a small, roving, robotic vehicle to supply data that is processed in real-time to safely navigate the surface of Mars. A simple, low-computation algorithm for constructing a 3-D navigational map of the Martian environment to be used by the rover is discussed

Glass transition and alpha-relaxation dynamics of thin films of labeled polystyrene

The glass transition temperature and relaxation dynamics of the segmental motions of thin films of polystyrene labeled with a dye, 4-[N-ethyl-N-(hydroxyethyl)]amino-4-nitraozobenzene (Disperse Red 1, DR1) are investigated using dielectric measurements. The dielectric relaxation strength of the DR1-labeled polystyrene is approximately 65 times larger than that of the unlabeled polystyrene above the glass transition, while there is almost no difference between them below the glass transition. The glass transition temperature of the DR1-labeled polystyrene can be determined as a crossover temperature at which the temperature coefficient of the electric capacitance changes from the value of the glassy state to that of the liquid state. The glass transition temperature of the DR1-labeled polystyrene decreases with decreasing film thickness in a reasonably similar manner to that of the unlabeled polystyrene thin films. The dielectric relaxation spectrum of the DR1-labeled polystyrene is also investigated. As thickness decreases, the $\alpha$-relaxation time becomes smaller and the distribution of the $\alpha$-relaxation times becomes broader. These results show that thin films of DR1-labeled polystyrene are a suitable system for investigating confinement effects of the glass transition dynamics using dielectric relaxation spectroscopy.Comment: 10 pages, 11 figures, 2 Table

Numerical Arc Segmentation Algorithm for a Radio Conference (NASARC), version 4.0: User's manual

The information in the NASARC (Version 4.0) Technical Manual (NASA-TM-101453) and NASARC (Version 4.0) User's Manual (NASA-TM-101454) relates to the state of Numerical Arc Segmentation Algorithm for a Radio Conference (NASARC) software development through November 1, 1988. The Technical Manual describes the NASARC concept and the algorithms used to implement the concept. The User's Manual provides information on computer system considerations, installation instructions, description of input files, and program operation instructions. Significant revisions were incorporated in the Version 4.0 software over prior versions. These revisions have further enhanced the modeling capabilities of the NASARC procedure and provide improved arrangements of predetermined arcs within the geostationary orbit. Array dimensions within the software were structured to fit within the currently available 12-megabyte memory capacity of the International Frequency Registration Board (IFRB) computer facility. A piecewise approach to predetermined arc generation in NASARC (Version 4.) allows worldwide planning problem scenarios to be accommodated within computer run time and memory constraints with enhanced likelihood and ease of solution

A Search for Variable Stars and Planetary Occultations in NGC2301 I: Techniques

We observed the young open cluster NGC 2301 for 14 nights in Feb. 2004 using the orthogonal transfer CCD camera (OPTIC). We used PSF shaping techniques ("square stars") during the observations allowing a larger dynamic range (4.5 magnitudes) of high photometric precision results ($\le$2 mmag) to be obtained. These results are better than similar observing campaigns using standard CCD imagers. This paper discusses our observational techniques and presents initial results for the variability statistics found in NGC 2301. Details of the variability statistics as functions of color, variability type, stellar type, and cluster location will appear in paper II