Yearly rates of myocardial infraction and infraction index from 1953 to 2016.

<p>(A) Number of papers retrieved for each year from a Scopus “all fields” search for “myocardial infraction”. (Accessed January 4, 2017). (B) The infraction index over the same time frame, calculated as a percentage of errors from the same search for “myocardial infarction”. (Accessed January 4, 2017).</p

The top ten journals ranked by infraction index, based on numbers of papers returning at least one “myocardial infraction” error in a Scopus search (1953 to 2017).

<p>The top ten journals ranked by infraction index, based on numbers of papers returning at least one “myocardial infraction” error in a Scopus search (1953 to 2017).</p

Morphology of ADSCs during adipogenic differentiation.

<p>At (A) Day 1 and (B) Day 7. Treatment conditions were (i) non-induced control media, (ii) adipogenic control, (iii) 10mM LiCl, (iv) 0.5μM BIO, (v) 100pg/mL sFRP4, and (vi) 1ng/mL sFRP4 in both (A) and (B). Scale bar = 10μM.</p

Dose response of ADSCs with Wnt regulators.

<p>In the presence of Wnt activators (A) LiCl, (B) BIO, and (C) Wnt antagonist sFRP4 (* p<0.05 and ** p<0.001).<b>iiiiiiivviv</b></p

Differentiation of ADSCs visualised by staining techniques.

<p>(A) Intracellular lipid globules of adipogenically differentiated ADSCs stained positively by Oil Red O (B) Calcium deposition of osteogenically differentiated ADSCs stained positively by Alizarin Red, (C) Mineralization of osteogenically differentiated ADSCs stained positively by Von Kossa, (D) Glycosaminoglycan deposition of chondrogenically differentiated ADSCs stained positively by Alcian blue, and (E) Fibroblast morphology of undifferentiated ADSCs. Scale bar = 10μM.</p

Morphology of ADSCs during adipogenic differentiation at Day 7.

<p>(A) Before and (B) after Oil red O staining. Treatment conditions were (i) non-induced control media, (ii) adipogenic control, (iii) 0.5μM BIO, (iv) 1ng/mL sFRP4, and (v) 0.5μM BIO + 1ng/mL sFRP4 in both (A) and (B). Scale bar = 10μM in (A) and 250μM in (B). (C) Quantification of the stained lipid droplets were performed using the eluted Oil red O stain via measuring absorbance at 510nm. The readings were normalised to background values of non-induced control ADSCs. The values of all treatment conditions were compared to the adipogenic control group (** p<0.001).</p

Oil Red O Staining and quantification on Day 7.

<p>(A) Microscopic observations of the stained lipid droplets. Treatment conditions were (i) non-induced control, (ii) adipogenic control, (iii) 10mM LiCl, (iv) 0.5μM BIO, (v) 100pg/mL sFRP4, and (vi) 1ng/mL sFRP4. Scale bar = 250μM (B) Quantification of the stained lipid droplets were performed using the eluted Oil red O stain via measuring absorbance at 510nm. The readings were normalised to background values of non-induced control ADSCs. The values of all treatment conditions were compared to the adipogenic control group (* p<0.05 and ** p<0.001).</p