Negative selection pressure analyses at specific codons of the VP1 region of Kenyan EV68 isolates.

<p>Codons that showed significant negative selection pressure are indicated in bold type.</p

Bayesian phylogenetic tree based on nucleotide sequences of partial VP1 genomic region of EV68 strains.

<p>Each reference strain sequence used in the analysis is represented by its GenBank accession number. The tree was estimated using MrBayes 3.2 with a general time-reversible (GTR) substitution model. Posterior probabilities support values are shown as percentages on each node. The scale bar indicates number of nucleotide substitutions per site. Kenyan isolates are shown in blue.</p

WebLogo comparisons of deduced amino acid sequences within BC and DE-loops of Kenyan EV68 isolates.

<p>Each logo is composed of stacks of symbols, one stack for each position in the sequence. The height of the stack indicates the relative frequency of each amino acid at that position. This graphical representation was created using WebLogo available at weblogo.berkeley.edu.</p

VP1 amino acid sequence alignment of Kenyan EV68 isolates alongside those retrieved from GenBank.

<p>Kenyan strains are designated starting with HEV and the other strains bear GenBank accession numbers. The BC and DE-loops are boxed.</p

Rainfall, temperature and relative humidity prevailing in Mombasa at the time of mosquito sampling in 2013 and 2014 (Source: Kenya Meteorological Department, 2013 and 2014).

<p>Rainfall, temperature and relative humidity prevailing in Mombasa at the time of mosquito sampling in 2013 and 2014 (Source: Kenya Meteorological Department, 2013 and 2014).</p

Rank distribution of wet containers sampled indoors and immature <i>Ae</i>. <i>aegypti</i> positivity by container type in Mombasa city during the outbreaks.

<p>Rank distribution of wet containers sampled indoors and immature <i>Ae</i>. <i>aegypti</i> positivity by container type in Mombasa city during the outbreaks.</p

The overall map of Kenya and Mombasa city showing the dengue outbreak sites where vector sampling was conducted.

<p>The overall map of Kenya and Mombasa city showing the dengue outbreak sites where vector sampling was conducted.</p

Mosquito species collected as adults by various methods combined.

<p>Mosquito species collected as adults by various methods combined.</p

Overall and site-specific House, Container and Breteau indices for Mombasa city during the outbreak.

<p>Overall and site-specific House, Container and Breteau indices for Mombasa city during the outbreak.</p

Overall rank distribution of immature <i>Ae</i>. <i>aegypti</i> totals by site and container type in Mombasa city during the outbreak.

<p>Overall rank distribution of immature <i>Ae</i>. <i>aegypti</i> totals by site and container type in Mombasa city during the outbreak.</p