Essential earth observation variables for high-level multi-scale indicators and policies

Several holistic approaches are based on the description of socio-ecological systems to address the sustainability challenge. Essential Variables (EVs) have the potential to support these approaches by describing the status of the Earth system through monitoring and modeling. The different classes of EVs can be organized along the environmental policy framework of Drivers, Pressures, States, Impacts and Responses. The EV concept represents an opportunity to strengthen monitoring systems by providing observations to seize the fundamental dimensions of the Earth system The Group on Earth Observation (GEO) is a partnership of 113 nations and 134 participating organizations in 2021 that are dedicated to making Earth Observation (EO) data available globally to inform about the state of the environment and enable data-driven decision processes. GEO is building the Global Earth Observation System of Systems, a set of coordinated and independent EO, information and processing systems that interoperate to provide access to EO for users in the public and private sectors. The progresses made in the development of various classes of EVs are described with their main policy targets, Internet links and key references The paper reviews the literature on EVs and describes the main contributions of the EU GEOEssential project to integrate EVs within the work plan of GEO in order to better address selected environmental policies and the SDGs. A new GEO-EVs community has been set to discuss about the current status of the EVs, exchange knowledge, experiences and assess the gaps to be solved in their communities of providers and users. A set of four traits characterizing an EV was put forward to describe the entire socio-ecological system of planet Earth: Essentiality, Evolvability, Unambiguity, and Feasibility. A workflow from the identification of EO data sources to the final visualization of SDG 15.3.1 indicators on land degradation is demonstrated, spanning through the use of different EVs, the definition of the knowledge base on this indicator, the implementation of the workflow in the VLab (a cloud-based processing infrastructure), the presentation of the outputs on a dedicated dashboard and the corresponding narrative through a story map. The concept of EV started in the climate sphere and spread to other domains of the earth system but less so in socio-economic activities. More work is therefore needed to converge on a common definition and criteria in order to complete the implementation of EVs in all GEO focus areas. EVs should screen the entire Earth's social-ecological system, providing a trusted and long-term foundation for interdisciplinary approaches such as ecological footprinting, planetary boundaries, disaster risk reduction, and nexus frameworks, as well as many other policy frameworks such as the SDG

High-performance liquid chromatography–tandem mass spectrometry in the identification and determination of phase I and phase II drug metabolites

Applications of tandem mass spectrometry (MS/MS) techniques coupled with high-performance liquid chromatography (HPLC) in the identification and determination of phase I and phase II drug metabolites are reviewed with an emphasis on recent papers published predominantly within the last 6 years (2002–2007) reporting the employment of atmospheric pressure ionization techniques as the most promising approach for a sensitive detection, positive identification and quantitation of metabolites in complex biological matrices. This review is devoted to in vitro and in vivo drug biotransformation in humans and animals. The first step preceding an HPLC-MS bioanalysis consists in the choice of suitable sample preparation procedures (biomatrix sampling, homogenization, internal standard addition, deproteination, centrifugation, extraction). The subsequent step is the right optimization of chromatographic conditions providing the required separation selectivity, analysis time and also good compatibility with the MS detection. This is usually not accessible without the employment of the parent drug and synthesized or isolated chemical standards of expected phase I and sometimes also phase II metabolites. The incorporation of additional detectors (photodiode-array UV, fluorescence, polarimetric and others) between the HPLC and MS instruments can result in valuable analytical information supplementing MS results. The relation among the structural changes caused by metabolic reactions and corresponding shifts in the retention behavior in reversed-phase systems is discussed as supporting information for identification of the metabolite. The first and basic step in the interpretation of mass spectra is always the molecular weight (MW) determination based on the presence of protonated molecules [M+H]+ and sometimes adducts with ammonium or alkali-metal ions, observed in the positive-ion full-scan mass spectra. The MW determination can be confirmed by the [M-H]- ion for metabolites providing a signal in negative-ion mass spectra. MS/MS is a worthy tool for further structural characterization because of the occurrence of characteristic fragment ions, either MSn analysis for studying the fragmentation patterns using trap-based analyzers or high mass accuracy measurements for elemental composition determination using time of flight based or Fourier transform mass analyzers. The correlation between typical functional groups found in phase I and phase II drug metabolites and corresponding neutral losses is generalized and illustrated for selected examples. The choice of a suitable ionization technique and polarity mode in relation to the metabolite structure is discussed as well

Grid based calibration of SWAT hydrological models

The calibration and execution of large hydrological models, such as SWAT (soil and water assessment tool), developed for large areas, high resolution, and huge input data, need not only quite a long execution time but also high computation resources. SWAT hydrological model supports studies and predictions of the impact of land management practices on water, sediment, and agricultural chemical yields in complex watersheds. The paper presents the gSWAT application as a web practical solution for environmental specialists to calibrate extensive hydrological models and to run scenarios, by hiding the complex control of processes and heterogeneous resources across the grid based high computation infrastructure. The paper highlights the basic functionalities of the gSWAT platform, and the features of the graphical user interface. The presentation is concerned with the development of working sessions, interactive control of calibration, direct and basic editing of parameters, process monitoring, and graphical and interactive visualization of the results. The experiments performed on different SWAT models and the obtained results argue the benefits brought by the grid parallel and distributed environment as a solution for the processing platform. All the instances of SWAT models used in the reported experiments have been developed through the enviroGRIDS project, targeting the Black Sea catchment area