Elucidating the domain architecture and functions of non-core RAG1: The capacity of a non-core zinc-binding domain to function in nuclear import and nucleic acid binding

<p>Abstract</p> <p>Background</p> <p>The repertoire of the antigen-binding receptors originates from the rearrangement of immunoglobulin and T-cell receptor genetic loci in a process known as V(D)J recombination. The initial site-specific DNA cleavage steps of this process are catalyzed by the lymphoid specific proteins RAG1 and RAG2. The majority of studies on RAG1 and RAG2 have focused on the minimal, core regions required for catalytic activity. Though not absolutely required, non-core regions of RAG1 and RAG2 have been shown to influence the efficiency and fidelity of the recombination reaction.</p> <p>Results</p> <p>Using a partial proteolysis approach in combination with bioinformatics analyses, we identified the domain boundaries of a structural domain that is present in the 380-residue N-terminal non-core region of RAG1. We term this domain the Central Non-core Domain (CND; residues 87-217).</p> <p>Conclusions</p> <p>We show how the CND alone, and in combination with other regions of non-core RAG1, functions in nuclear localization, zinc coordination, and interactions with nucleic acid. Together, these results demonstrate the multiple roles that the non-core region can play in the function of the full length protein.</p

A solar cycle of spacecraft anomalies due to internal charging

International audienceIt is important to appreciate how the morphology of internal charging of spacecraft systems, due to penetrating electrons, differs from that of the more common surface charging, due to electrons with lower energy. A specific and recurrent anomaly on a geostationary communication satellite has been tracked for ten years so that solar cycle and seasonal dependencies can be clearly established. Concurrent measurements of sunspot number, solar wind speed and 2-day >2 MeV electron fluence are presented to highlight pertinent space weather relationships, and the importance of understanding the complex particle interaction processes involved

Technical Note: Impact of nonlinearity on changing the a priori of trace gas profiles estimates from the Tropospheric Emission Spectrometer (TES)

International audienceNon-linear optimal estimates of atmospheric profiles from the Tropospheric Emission Spectrometer (TES) may contain a priori information that varies geographically, which is a confounding factor in the analysis and physical interpretation of an ensemble of profiles. A common strategy is to transform these profile estimates to a common prior using a linear operation thereby facilitating the interpretation of profile variability. However, this operation is dependent on the assumption of not worse than moderate non-linearity near the solution of the non-linear estimate. We examines the robustness of this assumption when exchanging the prior by comparing atmospheric retrievals from the Tropospheric Emission Spectrometer processed with a uniform prior with those processed with a variable prior and converted to a uniform prior following the non-linear retrieval. We find that linearly converting the prior following a non-linear retrieval is shown to have a minor effect on the results as compared to a non-linear retrieval using a uniform prior when compared to the expected total error, with less than 10% of the change in the prior ending up as unbiased fluctuations in the profile estimate results

Kinetics of catalysis with surface disorder

We study the effects of generalised surface disorder on the monomer-monomer model of heterogeneous catalysis, where disorder is implemented by allowing different adsorption rates for each lattice site. By mapping the system in the reaction-controlled limit onto a kinetic Ising model, we derive the rate equations for the one and two-spin correlation functions. There is good agreement between these equations and numerical simulations. We then study the inclusion of desorption of monomers from the substrate, first by both species and then by just one, and find exact time-dependent solutions for the one-spin correlation functions.Comment: LaTex, 19 pages, 1 figure included, requires epsf.st

Rotationally invariant family of L\'evy like random matrix ensembles

We introduce a family of rotationally invariant random matrix ensembles characterized by a parameter $\lambda$. While $\lambda=1$ corresponds to well-known critical ensembles, we show that $\lambda \ne 1$ describes "L\'evy like" ensembles, characterized by power law eigenvalue densities. For $\lambda > 1$ the density is bounded, as in Gaussian ensembles, but $\lambda <1$ describes ensembles characterized by densities with long tails. In particular, the model allows us to evaluate, in terms of a novel family of orthogonal polynomials, the eigenvalue correlations for L\'evy like ensembles. These correlations differ qualitatively from those in either the Gaussian or the critical ensembles.Comment: 9 pages, 5 figure