Analysis of GPS radio occultation data from the FORMOSAT-3/COSMIC and Metop/GRAS missions at CDAAC

This study investigates the noise level and mission-to-mission stability of Global Positioning System (GPS) radio occultation (RO) neutral atmospheric bending angle data at the UCAR COSMIC Data Analysis and Archive Center (CDAAC). Data are used from two independently developed RO instruments currently flying in orbit on the FORMOSAT-3/COSMIC (F3C) and Metop/GRAS (GNSS Receiver for Atmospheric Sounding) missions. The F3C 50 Hz RO data are post-processed with a single-difference excess atmospheric phase algorithm, and the Metop/GRAS 50 Hz closed loop and raw sampling (down-sampled from 1000 Hz to 50 Hz) data are processed with a zero-difference algorithm. The standard deviations of the F3C and Metop/GRAS bending angles from climatology between 60 and 80 km altitude from June–December 2009 are approximately 1.78 and 1.13 μrad, respectively. The F3C standard deviation reduces significantly to 1.44 μrad when single-difference processing uses GPS satellites on the same side of the spacecraft. The higher noise level for F3C bending angles can be explained by additional noise from the reference link phase data that are required with single-difference processing. The F3C and Metop/GRAS mean bending angles differences relative to climatology during the same six month period are statistically significant and have values of −0.05 and −0.02 μrad, respectively. A comparison of ~13 500 collocated F3C and Metop/GRAS bending angle profiles over this six month period shows a similar mean difference of ~0.02 ± 0.02 μrad between 30 and 60 km impact heights that is marginally significant. The observed mean difference between the F3C and Metop/GRAS bending angles of ~0.02–0.03 μrad is quite small and illustrates the high degree of re-produceability and mission independence of the GPS RO data at high altitudes. Collocated bending angles between two F3C satellites from early in the mission differ on average by up to 0.5% near the surface due to systematically lower signal-to-noise ratio for one of the satellites. Results from F3C and Metop/GRAS differences in the lower troposphere suggest the Metop/GRAS bending angles are negatively biased compared to F3C with a maximum of several percents near the surface in tropical regions. This bias is related to different tracking depths (deeper in F3C) and data gaps in Metop/GRAS which make it impossible to process the data from both missions in exactly the same way

MicroRNA-143 is a potential tumor suppressor targeting DNA methyltransferases 3a in colorectal cancer

Gastroenterology, 2009, v. 136 n. 5, suppl.1, p. A165, abstract no. 10692009 DDW (Digestive Disease Week) Abstract Supplement , AGA (American Gastroenterological Association) Institute Topic Forum, Oral sessions: Scientific sessions: Microrna and digestive cancers, Oral presentation no. 1069postprin

Pitfalls in the characterization of circulating and tissue-resident human γδ T cells

Dissection of the role and function of human γδ T cells and their heterogeneous subsets in cancer, inflammation, and auto-immune diseases is a growing and dynamic research field of increasing interest to the scientific community. Therefore, harmonization and standardization of techniques for the characterization of peripheral and tissue-resident γδ T cells is crucial to facilitate comparability between published and emerging research. The application of commercially available reagents to classify γδ T cells, in particular the combination of multiple Abs, is not always trouble-free, posing major demands on researchers entering this field. Occasionally, even entire γδ T cell subsets may remain undetected when certain Abs are combined in flow cytometric analysis with multicolor Ab panels, or might be lost during cell isolation procedures. Here, based on the recent literature and our own experience, we provide an overview of methods commonly employed for the phenotypic and functional characterization of human γδ T cells including advanced polychromatic flow cytometry, mass cytometry, immunohistochemistry, and magnetic cell isolation. We highlight potential pitfalls and discuss how to circumvent these obstacles

Can atopic eczema and psoriasis coexist? A systematic review and meta‐analysis

Importance: Previous studies report both co-existence and mutual exclusivity of atopic eczema (AE) and psoriasis, but these have not been appraised systematically. Knowledge of such disease association throws light on disease mechanisms and may influence therapeutic choices. Objective: To summarise evidence for AE and psoriasis occurring in the same person at the same point in time. Planned primary outcome was the incidence, prevalence or risk of psoriasis or eczema. Methods: Ovid MEDLINE and Ovid Embase were searched from inception to 1st February 2020. The search strategy was built around the key terms ‘atopic eczema’, ‘psoriasis’ and ‘co-existence’. Observational studies (cohort, case-control, cross-sectional and case-series) with a minimum of 10 consecutive patients. There were no restrictions on participants, geography or language. Studies were selected, data extracted and critically appraised independently by two reviewers. Data were extracted on the method of diagnosis: health professional (dermatologist, criteria, other), self-reported, not specified. Study quality was assessed using the validated Joanna Brigg’s Institute critical appraisal tool. A random-effects model was used to combine studies. Results: This review included 31 studies and 20 523 individuals with psoriasis and 1, 405 911 with AE. Eight studies reported the prevalence of AE in those with psoriasis and values ranged from 0.17% to 20%: the pooled prevalence was 2% (95%CI 1, 3). Seven studies reported the prevalence of psoriasis in those with AE and values ranged from 0.3% to 12.6%; the pooled prevalence was 2% (95%CI 1, 3). Ten studies were assessed as low risk of bias. Geographical area, method of diagnosis, setting and whether the assessment of diagnosis was blinded partly contributed to the heterogeneity. Conclusions This review provides some evidence for the co-existence of AE and psoriasis. Clinicians should be aware of co-existence at diagnosis, when selecting therapies and when reviewing poor response to treatment

The insertion/deletion (I/D) polymorphism in the Angiotensin-converting enzyme gene and cancer risk: a meta-analysis

<p>Abstract</p> <p>Background</p> <p>The insertion/deletion (I/D) polymorphism in the <it>Angiotensin-converting enzyme </it>(<it>ACE</it>) gene has been implicated in susceptibility to cancer, but a large number of studies have reported inconclusive results. The aim of this study is to assess the association between the I/D polymorphism in the <it>ACE </it>gene and cancer risk by meta-analysis.</p> <p>Methods</p> <p>A search was performed in Pubmed database, Embase database, Chinese Biomedical (CBM) database, China National Knowledge Infrastructure (CNKI) database and Weipu database, covering all studies until August 31, 2010. Statistical analysis was performed by using Revman4.2 and STATA 10.0.</p> <p>Results</p> <p>A total of 25 case-control studies comprising 3914 cancer patients and 11391 controls were identified. No significant association was found between the I/D polymorphism and over all cancer risks (OR = 0.88, 95%CI = 0.73-1.06, P = 0.17 for DD+DI vs. II). In the subgroup analysis by ethnicity, no significant association was found among Asians and Europeans for the comparison of DD+DI vs. II. In the subgroup analysis by cancer types, no significant associations were found among lung cancer, breast cancer, prostate cancer, colorectal cancer, gastric cancer for the comparison of DD+DI vs. II. Results from other comparative genetic models also indicated the lack of associations between this polymorphism and cancer risks.</p> <p>Conclusions</p> <p>This meta-analysis suggested that the <it>ACE </it>D/I polymorphism might not contribute to the risk of cancer.</p

Acute-Phase-HDL Remodeling by Heparan Sulfate Generates a Novel Lipoprotein with Exceptional Cholesterol Efflux Activity from Macrophages

During episodes of acute-inflammation high-density lipoproteins (HDL), the carrier of so-called good cholesterol, experiences a major change in apolipoprotein composition and becomes acute-phase HDL (AP-HDL). This altered, but physiologically important, HDL has an increased binding affinity for macrophages that is dependent on cell surface heparan sulfate (HS). While exploring the properties of AP-HDL∶HS interactions we discovered that HS caused significant remodeling of AP-HDL. The physical nature of this change in structure and its potential importance for cholesterol efflux from cholesterol-loaded macrophages was therefore investigated. In the presence of heparin, or HS, AP-HDL solutions at pH 5.2 became turbid within minutes. Analysis by centrifugation and gel electrophoresis indicated that AP-HDL was remodeled generating novel lipid poor particles composed only of apolipoprotein AI, which we designate β2. This remodeling is dependent on pH, glycosaminoglycan type, is promoted by Ca2+ and is independent of protease or lipase activity. Compared to HDL and AP-HDL, remodeled AP-HDL (S-HDL-SAA), containing β2 particles, demonstrated a 3-fold greater cholesterol efflux activity from cholesterol-loaded macrophage. Because the identified conditions causing this change in AP-HDL structure and function can exist physiologically at the surface of the macrophage, or in its endosomes, we postulate that AP-HDL contains latent functionalities that become apparent and active when it associates with macrophage cell surface/endosomal HS. In this way initial steps in the reverse cholesterol transport pathway are focused at sites of injury to mobilize cholesterol from macrophages that are actively participating in the phagocytosis of damaged membranes rich in cholesterol. The mechanism may also be of relevance to aspects of atherogenesis