The evolving definition of salivary gland stem cells

Dysfunction of the salivary gland and irreversible hyposalivation are the main side effects of radiotherapy treatment for head and neck cancer leading to a drastic decrease of the quality of life of the patients. Approaches aimed at regenerating damaged salivary glands have been proposed as means to provide long-term restoration of tissue function in the affected patients. In studies to elucidate salivary gland regenerative mechanisms, more and more evidence suggests that salivary gland stem/progenitor cell behavior, like many other adult tissues, does not follow that of the hard-wired professional stem cells of the hematopoietic system. In this review, we provide evidence showing that several cell types within the salivary gland epithelium can serve as stem/progenitor-like cells. While these cell populations seem to function mostly as lineage-restricted progenitors during homeostasis, we indicate that upon damage specific plasticity mechanisms might be activated to take part in regeneration of the tissue. In light of these insights, we provide an overview of how recent developments in the adult stem cell research field are changing our thinking of the definition of salivary gland stem cells and their potential plasticity upon damage. These new perspectives may have important implications on the development of new therapeutic approaches to rescue radiation-induced hyposalivation

Numerical and Experimental Analysis of the Pressure Signature for different High-Speed Trains

This paper describes a procedure for the validation of numerical codes able to reproduce the pressures in tunnel due to the passage of trains. In the first step, the parameters of the numerical code are set by matching the train-tunnel pressure signature measured during a single-passage of different types of train within the tunnel and in the second step, without changing the parameters, the crossing of two trains is simulated. Within the paper, the methodology is applied to the numerical mono-dimensional code DB-Tunnel while the experimental data are those collected during an experimental research programme carried out in the tunnel La Fornace, on the Italian high-speed railway from Roma to Firenze. The accuracy of the numerical code estimation is evaluated in terms of the maximum pressure generated in the tunnel by the train passing/crossing because this is the key parameter, according to the TSI standard for railway infrastructures

Simplified estimation of the train resistance parameters: full scale experimental tests and analysis

A CEN standard (EN 14067-4, 2005) describes the methodologies for the assessment of the running resistance of railway vehicles starting from full-scale test measurements. According to this standard, the speed dependent terms of the equation of Davis [1] have to be determined by means of coasting tests. In this paper, a new method to estimate the running resistance coefficients from a full-scale coasting test is proposed and compared with the two methods proposed in the CEN standard (the regression method and the speed history identification method). The main advantage of this new method is that it does not require the railway line characteristics to be known and it will be shown that the new method is able to evaluate the coefficients with an accuracy equivalent to that of the other methods considered

Experimental analysis of train slipstream in confined spaces

The train slipstream, i.e. the air velocities induced by the train, is one of the most important aerodynamic effects connected to railway vehicles because it has a direct impact on the safety of passengers on the platform and track workers along the railway line. In recent years, a lot of studies were performed to understand the development of this phenomenon in the open field and specific EU standards, the EN 14067-4 and the TSI (Technical Specifications for Interoperability) were issued. On the other hand, only few studies have been carried out to analyze the train passages in confined spaces (as tunnels, line sections with acoustic barriers, etc.), even if the first results of these analyses have shown that the confinement of the air causes more severe conditions regarding the speed of the air flow. This work aims at studying, through a full-scale experimental campaign, the effects of the flow confinement on the air speed caused by the train passage. In particular, the effects of different parameters, linked to the train i.e. the train type and length, the train speed and the measurement position, and linked to the infrastructure i.e. variations in the local infrastructure geometry, were analyzed

Full scale experimental tests to evaluate the train slipstream in tunnels

The train slipstream, i.e. the air velocity induced by the train, is one of the most important aerodynamic effects connected to railway vehicles because it has a direct impact on the safety of passengers on the platform and track workers along the railway line. In recent years, a lot of studies were performed to understand the development of this phenomenon in open field, and specific EU standards, the EN 14067–4 and the TSI were issued. Instead, only few studies have been carried out to analyse the train slipstream in confined spaces (as tunnels, line sections with acoustic barriers, etc.), even though the first results of these analyses have shown that the confinement of the air causes more severe conditions regarding the speed of the air flow. This work aims at studying, through a fullscale experimental campaign, the effects on the air flow speed caused by the train passage. The effects of different train parameters (i.e. train type and length, etc.) and infrastructure parameters (i.e. geometry variations) were analysed. Lastly, the results of a specific test considering the presence of a stationary train inside the tunnel while another train is passing are described, to simulate scenarios of ordinary railway traffic

Border Carbon Adjustments Based on Avoided Emissions: Addressing the Challenge of Its Design

Carbon pricing is an essential instrument to address climate change. However international differences in carbon control policies may cause not only carbon leakage but also competitiveness disadvantages. In this context, border carbon adjustments are a promising tool for discouraging these problems. But designing a real-world border carbon adjustment instrument implies to consider significant issues: technical feasibility, data availability, the risk of retaliation from developing countries, and its compatibility within the World Trade Organization legal framework. There are still no conclusive answers about a proper design. This paper is an attempt to address the above-mentioned challenges proposing a carbon border tax (CBT) based on avoided emissions. Such a CBT is applied at a product level and not at a sector level, and all international prices are deflated to guarantee that import like goods received a treatment similar to like domestic products. Using the WIOD, we simulate a CBT based on avoided emissions applied by the European Union, and we compare the results with a CBT based on embodied emissions. (c) 2017This work was supported by the Spanish Ministry of Economy and Competitiveness [grant numbers ECO2015-67524-R , ECO2015-68023 , and HAR2015-69620-C2-1-P ]; and the AGAUR of Catalonia's Government [grant number 2014SGR-950 ]

Tremor in motor neuron disease may be central rather than peripheral in origin

BACKGROUND AND PURPOSE: Motor neuron disease (MND) refers to a spectrum of degenerative diseases affecting motor neurons. Recent clinical and post-mortem observations have revealed considerable variability in the phenotype. Rhythmic involuntary oscillations of the hands during action, resembling tremor, can occur in MND, but their pathophysiology has not yet been investigated. METHODS: A total of 120 consecutive patients with MND were screened for tremor. Twelve patients with action tremor and no other movement disorders were found. Ten took part in the study. Tremor was recorded bilaterally using surface electromyography (EMG) and triaxial accelerometer, with and without a variable weight load. Power spectra of rectified EMG and accelerometric signal were calculated. To investigate a possible cerebellar involvement, eyeblink classic conditioning was performed in five patients. RESULTS: Action tremor was present in about 10% of our population. All patients showed distal postural tremor of low amplitude and constant frequency, bilateral with a small degree of asymmetry. Two also showed simple kinetic tremor. A peak at the EMG and accelerometric recordings ranging from 4 to 12 Hz was found in all patients. Loading did not change peak frequency in either the electromyographic or accelerometric power spectra. Compared with healthy volunteers, patients had a smaller number of conditioned responses during eyeblink classic conditioning. CONCLUSIONS: Our data suggest that patients with MND can present with action tremor of a central origin, possibly due to a cerebellar dysfunction. This evidence supports the novel idea of MND as a multisystem neurodegenerative disease and that action tremor can be part of this condition

SSADH variation in primates: Intra- and interspecific data on a gene with a potential role in human cognitive functions

In the present study we focus on the nucleotide and the inferred amino acid variation occurring in humans and other primate species for mitochondrial NAD(+)-dependent succinic semialdehyde dehydrogenase, a gene recently supposed to contribute to cognitive performance in humans. We determined 2527 bp of coding, intronic, and flanking sequences from chimpanzee, bonobo, gorilla, orangutan, gibbon, and macaque. We also resequenced the entire coding sequence on 39 independent chromosomes from Italian families. Four variable coding sites were genotyped in additional populations from Europe, Africa, and Asia. A test for constancy of the nonsynonymous vs. synonymous rates of nucleotide changes revealed that primates are characterized by largely variable d(N)/d(S) ratios. On a background of strong conservation, probably controlled by selective constraints, the lineage leading to humans showed a ratio increased to 0.42. Human polymorphic levels fall in the range reported for other genes, with a pattern of frequency and haplotype structure strongly suggestive of nonneutrality. The comparison with the primate sequences allowed inferring the ancestral state at all variable positions, suggesting that the c.538(C) allele and the associated functional variant is indeed a derived state that is proceeding to fixation. The unexpected pattern of human polymorphism compared to interspecific findings outlines the possibility of a recent positive selection on some variants relevant to new cognitive capabilities unique to humans

Aerodynamic loads in open air of high speed trains: Analysis of experimental data

The homologation of high-speed trains is a demanding and expensive procedure. In particular, the evaluation of train slipstream according to the standard TSI, 2008 is divided in two different test programmes: one concerning the workers at the trackside and the other studying the passengers standing on the platform. This paper presents some slipstream measurements performed on three high speed trains and a comparison between them. The objective is to investigate the slipstream on the platform and relate it to the flow measured at the trackside at the same height with respect to the top of the rail. This topic is currently under revision by the commission in charge of the TSI standard. Interesting evidence concerning the improvements of the aerodynamic performance of new-generation trains are highlighted

Ballast flight under high-speed trains: Wind tunnel full-scale experimental tests

The flying ballast phenomenon has become an important problem, in the last years, because of the development of high speed trains and the consequent increase of the speed up to 350. km/h. The problem is very complex since it is related to both railway infrastructure and train characteristics and since it involves mechanical and aerodynamic effects. The results of an experimental study carried out on the Italian high-speed railway and on a 1:1 real stretch of the railways in wind tunnel are presented in the paper. The study was aimed to analyze the effects of the height of the ballast level, the stone shape in the upper layer of the ballast and the compaction of the ballast bed on the problem. To this purpose a specific wind tunnel test rig was designed to reproduce in the wind tunnel a flow with the same average characteristics of the one measured on the real line, especially in the region close to the ballast and sleepers. Finally, starting from the results of these tests, possible countermeasures to ballast lifting on-set are proposed