METH CPP Preference Score, Locomotor Sensitization and Conditioned Hyperactivity.

<p><b>A)</b> Preference score in WT and mGlu5 KO mice at habituation and test. Preference score is defined as (time spent in the METH-paired compartment – time spent in the saline-paired compartment). <b>B)</b> Locomotor sensitization in WT and mGlu5 KO mice following acute saline or 2 mg/kg METH i.p. injection over 4 consecutive days. <b>C)</b> Conditioned hyperactivity in WT and mGlu5 KO mice during the CPP test. Data (mean±SEM) analysed using two- or three-way RM ANOVA, followed by one-way ANOVA split by the factor ‘genotype’ with a Bonferroni correction. In Figs <b>A)</b> and <b>C)</b>, significant effects of ‘day’ (<i>vs.</i> habituation) are represented by “#” (^###<i>p</i><.001); significant effects of ‘genotype’ (<i>vs</i>. WT on the same day) are represented by ‘*’ (**<i>p</i><.01). In Fig <b>B)</b>, significant effects of ‘day’ (vs. METH day 1) are indicated by ‘$’ for WT mice (^$$$<i>p</i><.001), ‘#’ for mGlu5 KO mice (^###<i>p</i><.001). Significant effects of ‘day’ (vs. saline day 1) are indicated by ‘*’ for WT mice (***<i>p</i><.001) and ‘?’ for mGlu5 KO mice (???<i>p</i><.001). A significant ‘day’ × ‘genotype’ interaction was present in both Fig. A) and C), suggesting <b>A)</b> a greater change degree of change from habituation to test in WT compared to mGlu5 KO mice, and <b>C)</b> a greater change degree of change from habituation to test in mGlu5 KO compared to WT mice. Abbreviations: Saline 1 - day 1 of saline treatment.</p

Lever Responses and Infusions during METH IVSA FR1 Acquisition, Self-administration, Extinction and Reinstatement.

<p>Stable FR1 infusions averaged over 5 days of stable self-administration; extinction data averaged over the final two days of extinction. Data presented as means±SEM. Data analysed using two- or three-way RM ANOVA, followed by one-way ANOVA split by corresponding factor, where appropriate. Significant effects of ‘lever type’ are indicated by hash symbols (<i>vs</i>. inactive lever; ^##<i>p</i><.01, ^###<i>p</i><.001); significant effects of ‘genotype’ are indicated by asterisks (*<i>p</i><.05); significant effects of ‘day’ are indicated by ‘$’ (^$<i>p</i><.05). Abbreviations: ALP - active lever press; FR1 - fixed ratio 1; ILP - inactive lever press.</p

Extinction and reinstatement of the operant response for sucrose.

<p><b>A)</b> Average active lever presses during Stable FR1 and the final 2 days of extinction. Both genotypes significantly decreased their active lever presses during both days of extinction. <b>B)</b> Average active lever presses during extinction and cue-induced reinstatement. Both genotypes increased their active lever pressing in the reinstatement test following extinction. Data (mean±SEM) analysed using two-way RM ANOVA, followed by one-way ANOVA split by the factor ‘genotype’ with a Bonferroni correction. Significant effects of ‘day’ (<i>vs.</i> Stable FR1) are represented by “#” (^##<i>p</i><.01; ^###<i>p</i><.001). Abbreviations: EXT - final extinction score; FR1– fixed ratio 1; Reinst - reinstatement.</p