In vitro digestion of emulsions: high spatiotemporal resolution using synchrotron SAXS

International audienceAlthough the biochemical processes of lipid digestion are well-known, the biophysical ones, responsible for the assembly of molecules into functional structures, lack studies resolving both time and space scales. About 35 years ago, the seminal microscopy study of Patton and Carey constituted a major advance to reach this goal. Nowadays, new perspectives arise from the availability of large facilities scattering techniques, able to monitor the dynamics of multi-scale assemblies with unprecedented resolutions. The present small angle X-ray scattering (SAXS) study focused on the roles of the emulsifier and triglyceride in the formation of lipid assemblies during emulsion digestion in vitro. By developing several interpretations of the data in the whole space range (qualitative, shape-dependent and shape-independent models), the characteristic size of the assemblies and their transition times were obtained, which depended on the triglyceride, but not on the emulsifier. The major assembly formed was found to be a spherical mixed micelle, but vesicle was also found to coexist throughout the digestion, although in a lower proportion. The quantitative determination of the sizes and proportions of these assemblies, as well as the evolution of these characteristics during digestion are precious information for nutritional sciences, as these assemblies are the vehicles of lipophilic nutrients and micronutrients towards their absorption site

The transverse asymmetry AT′A_{T'} from quasi-elastic 3He→(e⃗,e′)^3\overrightarrow{\rm He}(\vec{e},e') process and the neutron magnetic form factor.

We have measured the transverse asymmetry from inclusive scattering of longitudinally polarized electrons from polarized 3He nuclei at quasi-elastic kinematics in Hall A at Jefferson Lab with high statistical and systematic precision. The neutron magnetic form factor was extracted based on Faddeev calculations with an experimental uncertainty of less than 2 %