Exercise-induced signalling in different human muscle groups.

<p>Relative work-load during exercise-bout evaluated by A) % of maximal EMG and B) glycogen content in human soleus (SOL), lateral gastrocnemius (Gast) and vastus lateralis (VL) before and after inclined walking exercise at ∼70% VO2max. Measured phosphorylations of C) ERK1/2, D) AMPK and E) eEF2 normalised per total protein in SOL, Gast and VL before and after exercise. F) EMG-normalised phosphorylation per total protein in gastroc. vs. SOL. G) Representative blots. §/§§§ p<0.05/0.001 vs. soleus. *** p<0.001 exercise-effect vs. rest within muscle. ††† p<0.001 muscle-effect, n = 10.</p

Relative protein expression quantified from western blots in human soleus, lateral gastrocnemius (Gastroc.) and lateral vastus of the vastus lateralis muscle (VL). SOD2 showed a borderline significant trend (p = 0.07, 1-way ANOVA).

<p>n = 10.</p><p>*/**/***p<0.05/0.01/0.001 vs. soleus.</p

Overview of experimental protocol for pre-test day and experimental days 1 and 2.

<p>Overview of experimental protocol for pre-test day and experimental days 1 and 2.</p

Blood parameters measured as indicated at various time-points during an inclined walking exercise-bout at ∼70% VO2max.

<p>n = 10.</p><p>*/**/***p<0.05/0.01/0.001 vs. corresponding resting value.</p