Patients’ characteristics and differences in age distribution among patient subgroups.

<p>Patients’ characteristics and differences in age distribution among patient subgroups.</p

Age distribution, tobacco smoke habit and DNA ploidy status in oral potentially malignant disordes (OPMDs) and oral squamous cell carcinomas (OSCCs) patients.

<p>The bottom and the top of each box show the first and third quartile while the line inside the box represents the median (second quartile). The tips of the whiskers represent the minimum and the maximum data value. The number of patients for each category is indicated at the bottom of the corresponding box. The boxes corresponding to DNA diploid OPMDs/OSCCs are white while those corresponding to DNA aneuploid OPMDs/OSCCs have a striped pattern. Significant (MW test) P-values (P < 0.05) are indicated. The FDR q-value method was applied for multiple testing (n = 8) correction and the resulting q-values are indicated.</p

Relationship between ploidy status, age, and smoke habit, in OPMDs^a and OSCCs^b patients.

<p>Relationship between ploidy status, age, and smoke habit, in OPMDs<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0184425#t004fn001" target="_blank">^a</a> and OSCCs<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0184425#t004fn002" target="_blank">^b</a> patients.</p

Mean number of genomic aberrations (MNGA) per patient and age or smoking status in oral potentially malignant disordes (OPMDs) and oral squamous cell carcinomas (OSCCs) patients.

<p>The average number of aberrations per patient is represented as gray dots superimposed over boxes. These present a thick horizontal line indicating the median number per group, and delimitate the 25^th and 75^th percentile, while whiskers show the 95% confidence interval. A) average number of aberrations per patients’ age; B) average number of aberrations per patients’ smoking habit.</p

Patients’ tobacco consumption details (54 former and 76 current smokers).

<p>Patients’ tobacco consumption details (54 former and 76 current smokers).</p

Relationship between DNA aneuploidy and histological diagnosis in OPMDs/OSCCs.

<p>DNA diploid oral potentially malignant disorders (OPMDs) and oral squamous cell carcinomas (OSCCs) are shown in white stacked bars, while DNA aneuploid OPMDs/OSCCs are shown in black stacked bars. Non-dysplastic oral potentially malignant disorder (ND-OPMD); dysplastic oral potentially malignant disorder (D-OPMD). Significant P-values (P < 0.05) are shown. The FDR q-value method was applied for multiple testing (n = 4) correction; q-values < 0.1 are indicated in bold. N = 331 OPMDs/OSCCs; N = 224 ND-OPMDs; N = 34 D-OPMDs; N = 73 OSCCs.</p

Association of CNAs with ploidy status in OPMDs/OSCCs. P-values and q-values of the associations are the indicated.

<p>Association of CNAs with ploidy status in OPMDs/OSCCs. P-values and q-values of the associations are the indicated.</p

Number of bioptic samples used to isolate the nuclei suspension and perform hr DNA-FCM analysis subdivided by oral mucosa subsite and histology.

<p>¹ Non-dysplastic oral potentially malignant disorder.</p><p>² Dysplastic oral potentially malignant disorder.</p><p>³ Oral squamous cell carcinoma, OSCC.</p><p>Number of bioptic samples used to isolate the nuclei suspension and perform hr DNA-FCM analysis subdivided by oral mucosa subsite and histology.</p

Relationship between total CNAs and histological diagnosis in OPMDs/OSCCs.

<p>The bottom and the top of each box show the first and third quartile, respectively, while the line inside the box represents the median (second quartile). Please notice that when the median is not shown, its value = 0. The tips of the whiskers represent the minimum and the maximum data value. Oral potentially malignant disorders (OPMDs); oral squamous cell carcinomas (OSCCs); non-dysplastic oral potentially malignant disorders (ND-OPMDs); dysplastic oral potentially malignant disorders (D-OPMDs). CNAs are referred to as: total focal gains, TFG; total broad gains, TBG; total focal losses, TFL; total broad losses, TBL. Broad gains and broad losses correspond to gains or losses of more than half a chromosome arm, respectively. The boxes corresponding to the number of CNAs detected in ND-OPMD sites are shown in white; the boxes corresponding to the number of CNAs detected in mucosa sites affected by D-OPMDs and OSCCs are shown in gray. Significant MW P-values (P < 0.05) and their corresponding q-values are shown. The FDR q-value method was applied for multiple testing (n = 4) correction; q-values < 0.1 are indicated in bold. N = 94 OPMDs/OSCCs; N = 46 ND-OPMDs; N = 48 D-OPMDs/OSCCs.</p

Relationship between DNA aneuploidy, histological diagnosis in OPMDs/OSCCs and oral subsite TNG or BM.

<p>(A) shows the results of the analysis of oral potentially malignant disorders (OPMDs) and oral squamous cell carcinomas (OSCCs) limited to the tongue (TNG) or buccal mucosa (BM) mucosa; (B) shows the results of the analysis of TNG or BM from patients with OPMDs/OSCCs in multiple oral subsites. Non-dysplastic OPMDs (ND-OPMDs); dysplastic OPMDs (D-OPMDs). DNA diploid oral mucosa sites are shown in white stacked bars, while DNA aneuploid oral mucosa sites are shown in black stacked bars. Significant P-values (P < 0.05) are indicated. The FDR q-value method was applied for multiple testing (n = 4) correction; q-values < 0.1 are indicated in bold. (A) N = 163; N = 22 TNG and N = 82 BM ND-OPMDs; N = 43 TNG and N = 16 BM D-OPMDs/OSCCs. (B) N = 56; N = 18 including TNG and N = 24 including BM ND-OPMDs; N = 7 including TNG and N = 7 including BM D-OPMDs/OSCCs.</p