Chalcogenite- reducing activity assays in crude extracts of MF05 in anaerobiosis.

Enzymatic activity by MF05 crude extracts for (A) sulfite reduction through NADH or NADPH consumption, (B) selenite reduction, through Se0 formation, or (C) tellurite reduction through Te0 formation. The results represent the average of three independent trials ± SD. *, P < 0.05; *** P < 0.001.</p

Excitation and emission fluorescence scan of sulfite and cadmium checkerboards for <i>in vitro</i> synthesis of CdS QDs with NADH or NADPH by crude extracts of MF05 under anaerobic conditions.

Excitation (A and C) and emission (B and D) scan spectra of crude extracts of MF05 treated with Na2SO3 (S) and/or CdCl2 (Cd) in the presence of NADH (A and B) or NADPH (C and D). (TIF)</p

Release of H₂S by MF05 in M9 or M9* media, under aerobic or anaerobic conditions, and fluorescence tracing of CdS QDs formation at different times under anaerobic conditions.

(A) Integrated density of image processing of H2S release by MF05 cells grown in M9 or M9* medium under aerobic or anaerobic conditions with PbS filter papers. (B) CdS QDs formation after anaerobic growth of MF05 in M9* medium treated with 864 μM CdCl2 with their respective negative controls. (C) Excitation and (D) emission spectra scan of Cd-treated MF05 cells. λex: excitation with 590 nm emission wavelength; λem: emission with 370 nm excitation wavelength. For (A) the results represent the average of three independent trials ± SD ***, P < 0.001.</p

Clusters of Orthologous Groups (COGs).

COG functions comparison for E. coli BW25113 (blue) and MF05 (orange). (TIF)</p

Growth curves of <i>E</i>. <i>coli</i> BW25113 and MF05 in M9 or M9* media.

(A) Aerobic, (B) anaerobic E. coli BW25113, and (C) Aerobic, (D) MF05 cells grown in M9 (black circle) or M9* (inverted green triangle) medium. Each point represents the average of three independent trials ± SD. (TIF)</p

SEM EDX of MF05 cell extract samples treatments under anaerobic conditions.

Cells extracts were treated with (A) Na2SO3 + CdCl2, (B)Na2SeO3, (C) Na2TeO3, (D) HAuCl4, (E) AgNO3 or (F) CuSO4. (TIF)</p

Fluorescence emission of sulfite and cadmium checkerboards to check CdS QDs formation by MF05 cells with M9 or M9* medium under aerobic or anaerobic conditions.

Aerobic sulfite + CdCl2 checkerboards assays for E. coli BW25113 grown in (A) M9 or (C) M9* medium, and MF05 grown in (E) M9 or (F) M9* medium. Anaerobic sulfite + CdCl2 checkerboards assays for E. coli BW25113 in (B) M9 or (D) M9* medium, and MF05 grown in (F) M9 or (H) M9* medium. Fluorescence was monitored with 230 and 640 nm of excitation and emission wavelength, respectively. The results represent the average of 3 independent trials.</p

Heatmap of ANI analysis for MF05 against 12 different proteobacteria strains.

Nucleotide identity comparison between Acinetobacter schindleri ACE, Salmonella bongori NCTC 12419, S. enterica serovar Typhi CT18, Shigella sonnei BS1058, S. dysenteriae Sd197, S. flexneri 301, E. coli BW25113, E. coli MG1655, E. coli W3110, E. coli BL21 (DE3), E. coli O157:H7 Sakai, E. coli O104:H21 CSFSAN00236 and MF05.</p

Checkerboards fluorescence emission of Na₂SeO₃ and CdCl₂ or K₂TeO₃ and CdCl₂ under aerobic or anaerobic conditions for MF05 in M9* medium.

Fluorescence was monitored with 230 and 640 nm of excitation and emission wavelength, respectively. Na2SeO3 + CdCl2 in (A) aerobiosis or (B) anaerobiosis, and K2TeO3 + CdCl2 in (C) aerobiosis and (D) anaerobiosis. The results represent the average of three independent trials. (TIF)</p

TEM and SEM EDX of controls whole-cell assays in MF05.

Whole cells were (A) left untreated, or exposed to (B) only CdCl2 or (C) only Na2SO3.TEM (upper images) and SEM EDX (lower images) showed no formation of NP under these conditions. (TIF)</p