9 research outputs found
Additional file 2: of Comparative genomics of European avian pathogenic E. Coli (APEC)
Cumulative table. (XLSX 87Â kb
Details of strains used in the phenotypic microarray assay.
<p>Details of strains used in the phenotypic microarray assay.</p
Additional file 3: of Comparative genomics of European avian pathogenic E. Coli (APEC)
Hits. (XLSX 2118Â kb
Principal component analysis for metabolic profiles obtained from an analysis of the phenotypic microarray data set.
<p>ST131 strains are denoted by red blocks, and non-ST131 strains by blue blocks.</p
Cluster heat map showing the signal values of <i>E. coli</i> ST131 and non-ST131 isolates obtained from 190 biochemical tests using PM assay, with red showing no utilisation through to green showing high levels of utilisation.
<p>A UPGMA dendrogram informed by the metabolic profile is presented above the heatmap. ST131 strains are represented by red blocks, and non-ST131 strains by blue blocks.</p
Additional file 1: of Comparative genomics of European avian pathogenic E. Coli (APEC)
Assembly statistics. (XLSX 51Â kb
ACT comparisons of the (A) <i>idn</i> (B) <i>ydd</i> and (C) <i>asc</i> loci from CFT073 (upper genome) and the core ST131 genome (lower genome).
<p>ACT comparisons of the (A) <i>idn</i> (B) <i>ydd</i> and (C) <i>asc</i> loci from CFT073 (upper genome) and the core ST131 genome (lower genome).</p
Heat map showing a comparison of results obtained from testing 25 API biochemical substrates on <i>E. coli</i> ST131 and four major ExPEC STs representing the whole substrates used in phenotypic profiling assay.
<p>Darker shaded areas indicate higher percentage of strains capable of utilizing the substrate. The inability of ST131 to utilise 5KG, Dulcitol (DUL), DARA, cellobiose and esculin is highlighted by red circle.</p
Data_Sheet_1_A multi-country One Health foodborne outbreak simulation exercise: cross-sectoral cooperation, data sharing and communication.PDF
IntroductionThe awareness of scientists and policy makers regarding the requirement for an integrated One Health (OH) approach in responding to zoonoses has increased in recent years. However, there remains an overall inertia in relation to the implementation of practical cross-sector collaborations. Foodborne outbreaks of zoonotic diseases continue to affect the European population despite stringent regulations, evidencing the requirement for better ‘prevent, detect and response’ strategies. Response exercises play an essential role in the improvement of crisis management plans, providing the opportunity to test practical intervention methodologies in a controlled environment.MethodsThe One Health European Joint Programme simulation exercise (OHEJP SimEx) aimed at practicing the OH capacity and interoperability across public health, animal health and food safety sectors in a challenging outbreak scenario. The OHEJP SimEx was delivered through a sequence of scripts covering the different stages of a Salmonella outbreak investigation at a national level, involving both the human food chain and the raw pet feed industry.ResultsA total of 255 participants from 11 European countries (Belgium, Denmark, Estonia, Finland, France, Italy, Norway, Poland, Portugal, Sweden, the Netherlands) took part in national level two-day exercises during 2022. National evaluations identified common recommendations to countries aiming to improve their OH structure to establish formal communication channels between sectors, implement a common data sharing platform, harmonize laboratory procedures, and reinforce inter-laboratory networks within countries. The large proportion of participants (94%) indicated significant interest in pursuing a OH approach and desire to work more closely with other sectors.DiscussionThe OHEJP SimEx outcomes will assist policy makers in implementing a harmonized approach to cross-sector health-related topics, by highlighting the benefits of cooperation, identifying gaps in the current strategies and suggesting actions required to better address foodborne outbreaks. Furthermore, we summarize recommendations for future OH simulation exercises, which are essential to continually test, challenge and improve national OH strategies.</p