Datasets of DNA sequencing (Illumina) of ladybird beetles

The library names refer to:<div><br></div><div>46: Hippodamia convergens (TruSeq Nano, HiSeq 2500 Rapid Mode, 2x250 bp, insert size 550 bp, 250 cycle kit, 2% of the flow cell)</div><div><br></div><div>68: Harmonia axyridis (TruSeq Nano, HiSeq 2500 Rapid Mode, 2x250 bp, insert size 550 bp, 250 cycle kit, 2% of the flow cell)</div><div><br></div><div>BioA: Cycloneda sanguinea (TruSeq Nano, HiSeq 2500 Rapid Mode, 2x250 bp, insert size 550 bp, 250 cycle kit, 2% of the flow cell)</div><div><br></div><div>DebPool: several insect species multiplexed (TruSeq Nano, MiSeq, 2x250 bp, insert size 850 bp, 500 cycle kit, 15% flowcell)</div

Transcriptome-Based Identification of Highly Similar Odorant-Binding Proteins among Neotropical Stink Bugs and Their Egg Parasitoid - Fig 6

<p>Alignment of the deduced amino acid sequences of OBPs from the parasitoid <i>T</i>. <i>podisi</i> and the most similar OBPs obtained from GenBank by BLASTx: (a) TpodOBP1; (b) TpodOBP2; (c) TpodOBP3. Similarity is scored by matrix Blosum62 where the black color indicates 100% identity, darker grey 100% > identity ≥ 80%, lighter grey 80% > identity ≥ 60% and white color identity <60%. The sequence logo is at the top of the alignment. The amino acid percentage identity matrix is presented in Table H in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132286#pone.0132286.s002" target="_blank">S2 File</a>. The conserved Cys are indicated by sequence logo. The species names are abbreviated with four letters, and their full names with all accession numbers of the OBP amino acid sequences are provided in Table I in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132286#pone.0132286.s002" target="_blank">S2 File</a>.</p

Number of GO categories exclusive and common to each species: (a) biological process, and (b) molecular function.

<p>A species was considered to have the category if it had more than 10 transcripts. The radii of the circles are proportional to the number of categories.</p

Physico-chemical predictions for the putative full-length OBPs obtained from the target stink bugs (<i>E</i>. <i>heros</i>, <i>C</i>. <i>ubica</i> and <i>D</i>. <i>melacanthus</i>) and parasitoid (<i>T</i>. <i>podisi</i>).

<p>AA: Amino acid residue number; pI: Isoelectric point; MW: molecular weight (Da); Cys: cysteine; SignalP: signal peptide amino acid location. InterPro domains IPR023316 and SM00708, and families IPR006170 and PF01395. Unless otherwise indicated the “Conserved Cys” is six classical.</p

Most frequent biological processes (a) and molecular functions (b) categories from the antennae of 12 day-old virgin adults of stink bugs and whole body of 20 day-old parasitoid.

<p>The number of transcripts for a particular GO term (at lower hierarchy level) in each category was normalized by the total number of classified transcripts.</p

Phylogenetic relationships of: (a) target parasitoid putative OBPs (in red) and 215 putative other hymenopteran OBPs; (b) Detailed relationships of the putative TpodOBPs.

<p>The trees were constructed with MEGA 6.06 using a LG+G+I model. Values indicated at the nodes are bootstrap values based on 1,000 replicates presented with 50% cut-off bootstrap value for (a) and no cut-off for (b). The species names are abbreviated with four letters, and their full names with all accession numbers of the OBP amino acid sequences are provided in Table I in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132286#pone.0132286.s002" target="_blank">S2 File</a>.</p

Number of reads obtained from the RNA-Seq libraries and Illumina GAIIx sequencing for the stink bug antennae and whole-body parasitoid after quality control, and number of contigs assembled for each species.

<p>The minimum contig length was 300 bp for all samples. QC: quality control.</p

Match species, rank of the highest normalized number of hits for the target stink bugs and parasitoid, Kruskal-Wallis H value (H) and type I error probability (P).

<p>Match species in bold are significantly different among the target stink bugs and parasitoid.</p

Phylogenetic relationships of: (a) target stink bug putative OBPs (in red) and putative 185 other hemipteran OBPs; (b) Detailed relationships of the putative EherOBPs, CubiOBPs, and DimelOBP.

<p>The trees were constructed with MEGA 6.06 using a LG+G+I model. Values indicated at the nodes are bootstrap values based on 1,000 replicates presented with 50% cut-off bootstrap value for (a) and no cut-off for (b). The species names are abbreviated with four letters, and their full names with all accession numbers of the OBP amino acid sequences are provided in Table I in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0132286#pone.0132286.s002" target="_blank">S2 File</a>.</p

Alignment of the predicted tertiary structures of the full-length putative OBPs from the stink bugs <i>E</i>. <i>heros</i>, <i>C</i>. <i>ubica</i> and <i>D</i>. <i>melacanthus</i> and from the parasitoid <i>T</i>. <i>podisi</i>.

<p>Blue shows structural similarities and red shows dissimilarities according to the matrix Blosum60 for: (a) within EherOBPs; (b) within CubiOBPs; (c) between EherOBP3 and CubiOBP3; (d) between EherOBP6 and CubiOBP1; (e) within TpodOBPs; (f) between TpodOBP1 and EherOBP2; (g) between TpodOBP2 and EherOBP1. The structures were generated by I-TASSER server 4.2 and were oriented with the N-terminal to the right side.</p