MLVA16 dendogram of <i>B</i>. <i>abortus</i> isolates from different regions of Costa Rica.

<p>(A) Map of CR indicating the different regions from which <i>B</i>. <i>abortus</i> were isolated (circles). The color of the circles corresponds to the I-IV clusters, respectively. (B) MLVA16 dendogram constructed from the analysis of 107 <i>B</i>. <i>abortus</i> isolates (depicted in blue lines) are compared with MLVA16 of 219 <i>B</i>. <i>abortus</i> representative isolates from other latitudes (indicted in black lines). Clusters I to IV are indicted in the figure. S2 MLVA16 genetic profiles for the CR <i>B</i>. <i>abortus</i> isolates are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182380#pone.0182380.s002" target="_blank">S2 Table</a>.</p

Herd and bovine brucellosis reactors according to management system and sampling procedures^*.

<p>Herd and bovine brucellosis reactors according to management system and sampling procedures^{<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182380#t001fn001" target="_blank">*</a>}.</p

Herd prevalence in a non-random sample according to region and management system 2014–2016.

<p>Herd prevalence in a non-random sample according to region and management system 2014–2016.</p

Prevalence of bovine brucellosis in CR during five decades estimated by agglutination tests.

<p>The prevalence from 1965–1969 was assessed by tube agglutination; the prevalence from 1970–1986 was assessed by card test in combination with 2- mercaptoethanol agglutination assay; the prevalence from 1987–1994 were estimated by RBT [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182380#pone.0182380.ref010" target="_blank">10</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0182380#pone.0182380.ref014" target="_blank">14</a>]. Prevalence values from 2012–2016 assessed by RBT are from this work.</p

Herd prevalence in a random sample according to region and management system 2012–2013.

<p>Herd prevalence in a random sample according to region and management system 2012–2013.</p

Numbers of ovine and caprine herds and numbers of animals by geographical region in Costa Rica (2015).

<p>Numbers of ovine and caprine herds and numbers of animals by geographical region in Costa Rica (2015).</p

Sampling of animal stocks, in the six regions of CR.

<p>The epidemiological regions are as follow: 1, Northern Huetar; 2, Central; 3, Brunca; 4, Chorotega; 5, Caribbean Huetar; 6, Central Pacific. Each red dot represents an animal stock facility.</p