1 research outputs found
Two-Photon Optical Interrogation of Individual Dendritic Spines with Caged Dopamine
We introduce a novel caged dopamine
compound (RuBi-Dopa) based
on ruthenium photochemistry. RuBi-Dopa has a high uncaging efficiency
and can be released with visible (blue-green) and IR light in a two-photon
regime. We combine two-photon photorelease of RuBi-Dopa with two-photon
calcium imaging for an optical imaging and manipulation of dendritic
spines in living brain slices, demonstrating that spines can express
functional dopamine receptors. This novel compound allows mapping
of functional dopamine receptors in living brain tissue with exquisite
spatial resolution