Physiological and biochemical adaptation to salinity in wild halophytes suitable for Mediterranean agriculture

Due to the accelerating processes of soil salinization and shortage of fresh water, the practice of saline agriculture is gaining momentum in many areas of the world. However, there are some concerns that using saline water for irrigation may be non-environmentally sustainable, with potential to cause irreversible soil degradation. In addition, there is a lack of information on the morphological, physiological, and biochemical changes that can occur in plants when irrigated with saline water. In light of the above, the major aim of this work was to investigate the effects of a range of water salinity levels and irrigation regimes on the performances of salt tolerant species promising as future crop plants for saline agriculture. The following objectives were addressed: To determine the effects of different water regimes (leaching irrigation vs. no leaching irrigation) with water at increasing salinity concentrations on the growth, ion accumulation and water relations of Sorghum bicolor plants grown under saline soil conditions. To describe the germination response of Salicornia europaea seeds across a wide range of water salinity levels through six reliable indices for screening salinity tolerance at the seed germination stage. To explore the different physiological responses of six wild halophytes commonly found in the Mediterranean area (Artemisia absinthium, Artemisia vulgaris, Atriplex halimus, Chenopodium album, Salsola komarovii, and Sanguisorba minor), and rank their tolerance after exposure to growing levels of water salinity. To identify the main adaptation mechanisms that distinguish C3 from C4 halophytes when exposed to increasing salinity in the growth media, through a comparative study between the C3 species Atriplex hortensis and the C4 species Atriplex halimus. To identify the main adaptation mechanisms that distinguish annual from perennial halophytes when exposed to severe conditions of salinity and drought, through a comparative analysis between two annual Salicornia spp. and the perennial Sarcocornia fruticosa

Growth, yield and quality attributes of guar (Cyamopsis tetragonoloba L.) genotypes grown under different planting dates in a semi-arid region of Pakistan

Guar (Cyamopsis tetragonoloba L.) is grown in semi-arid regions worldwide as a forage, vegetable, and green manure crop. An experiment was conducted to evaluate growth, forage yield and nutritional quality of guar genotypes grown under different sowing dates. To this end, seven genotypes, viz., Desi Punjab, Farmi Punjab, Desi Sindh, Farmi Sindh, BR-90, BR-2017 and Baluchistan were grown at three sowing times (mid-May, late-May and early-June) during the two years 2020 and 2021. Results showed that maximum leaf area and pods per plant were recorded in genotype Farmi Punjab, while maximum fresh and dry forage yield were archived in genotype ‘BR-90’. Similarly, maximum crude protein in the forage and gum content in mature seeds, were also recorded in BR-90. In addition, mid-May was proved to be the best sowing time for guar genotypes studied in view of achieving maximum morphological traits, fresh and dry forage yield and forage quality, compared to later planting times. Moreover, late sowing caused reduction in yield and other related attributes. It is perceived that synthetic guar variety BR-90 and planting time at mid-May are the best for semi-arid region of Punjab, Pakistan

Effects of soil and water salinity in a sorghum pot experiment

Salinity is associated with reduced water availability because of the drop in soil water potential. Under salt stress, sorghum can lower leaf water potential to maintain water uptake and cell hydration, resulting in osmotic adjustment (Yang et al., 1990; Weimberg et al., 1984). The objective of this investigation was to determine the effects on sorghum growth and leaf water status at varying levels of soil and water salinit

Data on seed germination using LED versus fluorescent light under growth chamber conditions

The present investigation attempted to assess the influence o ftwo light sources, LED versus fluorescent light, on seed germination of nine aromatic species belonging to the genus Artemisia, Atriplex, Chenopodium, Salicornia, Sanguisorba, Portulaca and Rosmarinus. Pre-germination test was carried out in petri dishes, evidencing the need to overcome seed dormancy through cold stratification in Salicornia europaea. Thereafter, seeds were germinated in small trays with peat moss substrate in two growth chambers illuminated with eithe rLED or fluorescent light featuring similar photosynthetic photon flux density. Germination lasted 20 days, during which time five indexes of germination performance (germination percentage, speed of germination, germinatio nenergy, germination rate index, and mean daily germination) were evaluated. At the end, shoo tlength and seedling fresh weight were assessed as early growth traits. Data are made available to allow critical evaluation of experimental outcome

mandala crop model

This software refers to the submission " Playing a crop simulation model using symbols and sounds: the 'mandala' ". The article is currently under consideration by the journal in silico Plants. mandala.vl is the vvvv patch, the README.md file provides instructions to set the sounds of the mandala the folder mandalamodel.zip contains the following folders: cropModelvvvv: the C# source code of the crop model. The solution file is cropModelvvvv.sln files: the csv files soil and crop parameters instrument mandala: the file sounds of mandala runner: the console application to run the crop model weather: the csv files with input weather dataThis research was performed using in part funding from the Agritech National Research Center and received funding from the European Union Next-Generation EU (PIANO NAZIONALE DI RIPRESA E RESILIENZA (PNRR)—MISSIONE 4 COMPONENTE 2, INVESTIMENTO 1.4—D.D. 1032 17/06/2022, CN00000022)

Physiological Adaptation to Water Salinity in Six Wild Halophytes Suitable for Mediterranean Agriculture

Owing to the high interspecific biodiversity, halophytes have been regarded as a tool for understanding salt tolerance mechanisms in plants in view of their adaptation to climate change. The present study addressed the physiological response to salinity of six halophyte species common in the Mediterranean area: Artemisia absinthium, Artemisia vulgaris, Atriplex halimus, Chenopodium album, Salsola komarovii, and Sanguisorba minor. A 161-day pot experiment was conducted, watering the plants with solutions at increasing NaCl concentration (control, 100, 200, 300 and 600 mM). Fresh weight (FW), leaf stomatal conductance (GS), relative water content (RWC) and water potential (WP) were measured. A principal component analysis (PCA) was used to describe the relationships involving the variables that accounted for data variance. A. halimus was shown to be the species most resilient to salinity, being able to maintain FW up to 300 mM, and RWC and WP up to 600 mM; it was followed by C. album. Compared to them, A. vulgaris and S. komarovii showed intermediate performances, achieving the highest FW (A. vulgaris) and GS (S. komarovii) under salinity. Lastly, S. minor and A. absinthium exhibited the most severe effects with a steep drop in GS and RWC. Lower WP values appeared to be associated with best halophyte performances under the highest salinity levels, i.e., 300 and 600 mM NaCl

Recovery from Salinity and Drought Stress in the Perennial Sarcocornia fruticosa vs. the Annual Salicornia europaea and S. veneta

Current agricultural problems, such as the decline of freshwater and fertile land, foster saline agriculture development. Salicornia and Sarcocornia species, with a long history of human consumption, are ideal models for developing halophyte crops. A greenhouse experiment was set up to compare the response of the perennial Sarcocornia fruticosa and the two annual Salicornia europaea and S. veneta to 30 days of salt stress (watering with 700 mM NaCl) and water deficit (complete withholding of irrigation) separate treatments, followed by 15 days of recovery. The three species showed high tolerance to salt stress, based on the accumulation of ions (Na+, Cl−, Ca2+) in the shoots and the synthesis of organic osmolytes. These defence mechanisms were partly constitutive, as active ion transport to the shoots and high levels of glycine betaine were also observed in non-stressed plants. The three halophytes were sensitive to water stress, albeit S. fruticosa to a lesser extent. In fact, S. fruticosa showed a lower reduction in shoot fresh weight than S. europaea or S. veneta, no degradation of photosynthetic pigments, a significant increase in glycine betaine contents, and full recovery after the water stress treatment. The observed differences could be due to a better adaptation of S. fruticosa to a drier natural habitat, as compared to the two Salicornia species. However, a more gradual stress-induced senescence in the perennial S. fruticosa may contribute to greater drought tolerance in this species

Effect of Recurrent Salt and Drought Stress Treatments on the Endangered Halophyte <i>Limonium angustebracteatum</i> Erben

Limonium angustebracteatum is an endemic halophyte from the Spanish Mediterranean coastal salt marshes. To investigate this species’ ability to cope with recurrent drought and salt stress, one-year-old plants were subjected to two salt stress treatments (watering with 0.5 and 1 M NaCl solutions), one water stress treatment (complete irrigation withholding), or watered with non-saline water for the control, across three phases: first stress (30 days), recovery from both stresses (15 days), and second stress (15 days). Growth and biochemical parameters were determined after each period. The plants showed high salt tolerance but were sensitive to water deficit, as shown by the decrease in leaf fresh weight and water content, root water content, and photosynthetic pigments levels in response to the first water stress; then, they were restored to the respective control values upon recovery. Salt tolerance was partly based on the accumulation of Na+, Cl− and Ca2+ in the roots and predominantly in the leaves; ion levels also decreased to control values during recovery. Organic osmolytes (proline and total soluble sugars), oxidative stress markers (malondialdehyde and H2O2), and antioxidant compounds (total phenolic compounds and flavonoids) increased by various degrees under the first salt and water stress treatments, and declined after recovery. The analysed variables increased again, but generally to a lesser extent, during the second stress phase, suggesting the occurrence of stress acclimation acquired by the activation of defence mechanisms during the first stress period