Additional file 1: Table S1. of Immune profile of an atypical EAE model in marmoset monkeys immunized with recombinant human myelin oligodendrocyte glycoprotein in incomplete Freund’s adjuvant

Overview of post-mortem MRI parameters of each marmoset monkey of experiment B. (DOC 77 kb

One route of SC migration: the blood vessels.

<p>SC grafted in the spinal cord parenchyma are often localized in white matter around blood vessels (asterisks), evidenced with anti-laminin antibody (blue). While at 7 days SC are present close to the blood vessel wall (A), at 21 days they are embedded in the perivascular space but remote from the vascular wall (B).</p

Comparison of the remyelination potential of GFP-SCs grafted in the cisterna magna or the spinal cord.

<p>Twenty one days after SC delivery in the cisterma magna (A) or the spinal cord (B–F), exogenous remyelination was assessed using an anti-P0 antibody (red). P0 positivity colocalized with GFP-SCs (C, confocal microscopy), more frequently after spinal cord graft than cisterna magna delivery. C is a higher magnification of B and corresponds to the lesion detected on an adjacent section and illustrated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0042667#pone-0042667-g005" target="_blank">Fig 5C</a>. GFP-SCs produce P0+ myelin (red D, E) that surrounds neurofilament (NF) + axons (blue D, F). Inserts are higher magnifications of dashed boxes.</p

Distribution of GFP labeled SC after delivery in the cisterna magna and the spinal cord.

<p>GFP-SC (green) are detected both in the cerebellar parenchyma (A, B) and meninges (C) 7 days (A–C) after cisterna magna delivery as well as 21 days after in the proximal spinal cord (D). GFP-SCs grafted in the spinal cord parenchyma (E, F) are concentrated around blood vessels, some migrate away from the graft toward a lesion (L) identified by MOG immunostaining through white matter (E, arrows). (F) Same field illustrating GFP-SCs, inset is a higher magnification.</p

Interaction of GFP-SCs with myelin and astrocytes after delivery in the cisterna magna or the spinal cord parenchyma.

<p>Combined detection of MOG, GFAP and GFP on cryostat sections showed that GFP-SCs grafted in the cisterna magna (A, D) or the spinal cord (B,C, E, F) are found in demyelinated lesions (delineated by dashed lines) detected by MOG immunostaining (red) both after 7 days (B) and 21 days (A, C) after delivery. GFAP+ astrocytes (blue) interact with GFP-labeled SCs in the cisterna magna (D) and the spinal cord (E, F); 7 days (E) or 21 days (F) after spinal cord graft or 21 days after cisterna magna graft (D).</p

Illustrations of a focal spinal cord lesion of MOG induced EAE in the DA rat.

<p>LFB staining shows a focal demyelinated lesion in the dorsal funiculus with myelin loss (A–B). ED-1 immunostaining highlights the presence of macrophages/microglia cells within the lesion (C). MBP immunohistochemistry illustrates the loss of myelin and the presence of myelin-laden macrophages (close up in insert) is indicative of recent myelin phagocytosis (D).</p

<i>In vitro</i> characterization of transduced SC-GFP.

<p>The majority of SC-GFP (green, A, C) express the SC marker p75 (red, B, C).</p