Cantilever micro-rheometer for the dcharacterization of sugar solutions

The volume required for the rheological characterization of fluids can be minimized by using micromechanical cantilevers as viscosity sensors. Here, a simple measurement tool for the characterization of sugar solutions is proposed. The sensor consists of a micromechanical cantilever as used in an atomic force microscopy which is integrated into a closed fluid handling system. Fluid properties are derived from an analysis of the power spectral density of the fluctuations of the cantilever deflection signal. The data acquisition system is operated with standard consumer computer components, which limits the costs for the hardware. Measurements with different sugar solutions indicate that the sensor system provides reliable viscosity values for sugar concentrations as they occur in biological systems. The viscosities of the sugar solutions could be evaluated with an error smaller than 5%

Non-leaching antimicrobial surfaces through polydopamine bio-inspired coating of quaternary ammonium salts or an ultrashort antimicrobial lipopeptide.

Bacterial fouling on surfaces significantly increases the resistance of bacteria toward antibiotics, which leads to medical complications and a corresponding financial burden. Here, we report on a general and robust technique for facile modification of various surfaces with different antibacterial agents. Our approach in this study was inspired by the strong adhesion of mussel adhesion proteins (MAPs) to many types of surfaces, including metals, polymers, and inorganic materials. Thus, glass and polymeric slides were dip-coated with dopamine, as a MAP mimic, and the resulting surfaces were characterized. The reactivity of dopamine-coated surfaces toward nucleophilic addition was then confirmed by reacting them with fluorescent probes containing either a free amino or a free thiol group. Laser scanning confocal microscopy (LSCM), X-ray photoelectron spectroscopy (XPS), confocal Raman microscopy, matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectroscopy, and cyclic voltammetry studies collectively suggested that the probes had covalently attached to the surfaces. Fabrication of dopamine-coated surfaces with an antibacterial quaternary amine or an ultrashort lipopeptide analog generated surfaces that effectively kill Escherichia coli and Staphylococcus aureuscells on contact. Moreover, minimal leaching of the fabricated agent was detected after prolonged incubation. This technique could be further developed to a "paint-like" or self-assembling monolayer-like procedure for the preparation of antibacterial surfaces on various materials

Zero Field Assembly of Long Magnetic Dipolar Chains in 2D Polymer Nanocomposite Films

The existence of magnetic dipolar nanoparticle chains at zero field has been predicted theoretically for decades, but these structures are rarely observed experimentally. A prerequisite is a permanent magnetic moment on the particles forming the chain. Here we report on the observation of magnetic dipolar chains of spherical iron oxide nanoparticles with a diameter of \SI{12.8}{\nano\meter}. The nanoparticles are embedded in an ultrathin polymer film. Due to the high viscosity of the polymer matrix, the dominating aggregation mechanism is driven by dipolar interactions. Smaller iron oxide nanoparticles (\SI{9.4}{\nano\meter}) show no permanent magnetic moment and do not form chains but compact aggregates. Mixed monolayers of different iron oxide nanoparticles and polymer at the air-water interface are characterized by Langmuir isotherms and in-situ X-ray reflectometry (XRR). The combination of the particles with a polymer leads to a stable polymer nanocomposite film at the air-water interface. XRR experiments show that nanoparticles are immersed in a thin polymer matrix of \SI{3}{\nano\meter}. Using atomic force microscopy (AFM) on Langmuir-Blodgett films, we measure the lateral distribution of particles in the film. An analysis of single structures within transferred films results in fractal dimensions that are in excellent agreement with 2D simulations.Comment: 34 pages, 9 figures, electronic supporting information, TU Darmstad

Zero Field Assembly of Long Magnetic Dipolar Chains in 2D Polymer Nanocomposite Films

The existence of magnetic dipolar nanoparticle chains at zero field has been predicted theoretically for decades, but these structures are rarely observed experimentally. A prerequisite is a permanent magnetic moment on the particles forming the chain. Here we report on the observation of magnetic dipolar chains of spherical iron oxide nanoparticles with a diameter of \SI{12.8}{\nano\meter}. The nanoparticles are embedded in an ultrathin polymer film. Due to the high viscosity of the polymer matrix, the dominating aggregation mechanism is driven by dipolar interactions. Smaller iron oxide nanoparticles (\SI{9.4}{\nano\meter}) show no permanent magnetic moment and do not form chains but compact aggregates. Mixed monolayers of different iron oxide nanoparticles and polymer at the air-water interface are characterized by Langmuir isotherms and in-situ X-ray reflectometry (XRR). The combination of the particles with a polymer leads to a stable polymer nanocomposite film at the air-water interface. XRR experiments show that nanoparticles are immersed in a thin polymer matrix of \SI{3}{\nano\meter}. Using atomic force microscopy (AFM) on Langmuir-Blodgett films, we measure the lateral distribution of particles in the film. An analysis of single structures within transferred films results in fractal dimensions that are in excellent agreement with 2D simulations.Comment: 34 pages, 9 figures, electronic supporting information, TU Darmstad

Nanostructure and mechanics of mummified type I collagen from the 5300-year-old Tyrolean Iceman

Skin protects the body from pathogens and degradation. Mummified skin in particular is extremely resistant to decomposition. External influences or the action of micro-organisms, however, can degrade the connective tissue and lay the subjacent tissue open. To determine the degree of tissue preservation in mummified human skin and, in particular, the reason for its durability, we investigated the structural integrity of its main protein, type I collagen. We extracted samples from the Neolithic glacier mummy known as ‘the Iceman’. Atomic force microscopy (AFM) revealed collagen fibrils that had characteristic banding patterns of 69 ± 5 nm periodicity. Both the microstructure and the ultrastructure of dermal collagen bundles and fibrils were largely unaltered and extremely well preserved by the natural conservation process. Raman spectra of the ancient collagen indicated that there were no significant modifications in the molecular structure. However, AFM nanoindentation measurements showed slight changes in the mechanical behaviour of the fibrils. Young's modulus of single mummified fibrils was 4.1 ± 1.1 GPa, whereas the elasticity of recent collagen averages 3.2 ± 1.0 GPa. The excellent preservation of the collagen indicates that dehydration owing to freeze-drying of the collagen is the main process in mummification and that the influence of the degradation processes can be addressed, even after 5300 years

Durability Characterization of Mechanical Interfaces in Solar Sail Membrane Structures

The construction of a solar sail from commercially available metallized film presents several challenges. The solar sail membrane is made by seaming together strips of metallized polymer film. This requires seaming together a preselected width and thickness of a base material into the required geometry, and folding the assembled sail membranes into a small stowage volume prior to launch. The sail membranes must have additional features for connecting to rigid structural elements (e.g., sail booms) and must be electrically grounded to the spacecraft bus to prevent charge build up. Space durability of the material and mechanical interfaces of the sail membrane assemblies will be critical for the success of any solar sail mission. In this study, interfaces of polymer/metal joints in a representative solar sail membrane assembly were tested to ensure that the adhesive interfaces and the fastening grommets could withstand the temperature range and expected loads required for mission success. Various adhesion methods, such as surface treatment, commercial adhesives, and fastening systems, were experimentally evaluated and will be discussed

The Effects of Pictorial Realism, Delay of Visual Feedback, and Observer Interactivity on the Subjective Sense of Presence

Two experiments examined the effects of pictorial realism, observer interactivity, and delay of visual feedback on the sense of presence. Subjects were presented pairs of virtual enviornments (a simulated driving task) that differed in one or more ways from each other. After subjects had completed the second member of each pair they reported which of the two had produced the greater amount of presence and indicated the size of this difference by means of a 1-100 scale. As predicted, realism and interactivity increased presence while delay of visual feedback diminished it. According to subjects\u27 verbal responses to a postexperiment interview, pictorial realism was the least influential of the three variables examined. Further, although some subjects reported an increase in the sense of presence over the course of the experiment, most said it reamined unchanged or became weaker

LRRML: a conformational database and an XML description of leucine-rich repeats (LRRs)

Background: Leucine-rich repeats (LRRs) are present in more than 6000 proteins. They are found in organisms ranging from viruses to eukaryotes and play an important role in protein-ligand interactions. To date, more than one hundred crystal structures of LRR containing proteins have been determined. This knowledge has increased our ability to use the crystal structures as templates to model LRR proteins with unknown structures. Since the individual three-dimensional LRR structures are not directly available from the established databases and since there are only a few detailed annotations for them, a conformational LRR database useful for homology modeling of LRR proteins is desirable. Description: We developed LRRML, a conformational database and an extensible markup language (XML) description of LRRs. The release 0.2 contains 1261 individual LRR structures, which were identified from 112 PDB structures and annotated manually. An XML structure was defined to exchange and store the LRRs. LRRML provides a source for homology modeling and structural analysis of LRR proteins. In order to demonstrate the capabilities of the database we modeled the mouse Toll-like receptor 3 (TLR3) by multiple templates homology modeling and compared the result with the crystal structure. Conclusion: LRRML is an information source for investigators involved in both theoretical and applied research on LRR proteins. It is available at http://zeus.krist.geo.uni-muenchen.de/similar to lrrml

Guided Neuronal Growth on Arrays of Biofunctionalized GaAs/InGaAs Semiconductor Microtubes

We demonstrate embedded growth of cortical mouse neurons in dense arrays of semiconductor microtubes. The microtubes, fabricated from a strained GaAs/InGaAs heterostructure, guide axon growth through them and enable electrical and optical probing of propagating action potentials. The coaxial nature of the microtubes -- similar to myelin -- is expected to enhance the signal transduction along the axon. We present a technique of suppressing arsenic toxicity and prove the success of this technique by overgrowing neuronal mouse cells.Comment: 3 pages, 4 figure

Ionization States and Plasma Structures of Mixed-morphology SNRs Observed with ASCA

We present the results of a systematic study using ASCA of the ionization state for six ``mixed-morphology'' supernova emnants (MMSNRs): IC 443, W49B, W28, W44, 3C391, and Kes 27. MMSNRs show centrally filled thermal X-ray emission, which contrasts to shell-like radio morphology, a set of haracteristics at odds with the standard model of SNR evolution (e.g., the Sedov model). We have therefore studied the evolution of the MMSNRs from the ionization conditions inferred from the X-ray spectra, independent of X-ray morphology. We find highly ionized plasmas approaching ionization equilibrium in all the mmsnrs. The degree of ionization is systematically higher than the plasma usually seen in shell-like SNRs. Radial temperature gradients are also observed in five remnants, with cooler plasma toward the limb. In IC 443 and W49B, we find a plasma structure consistent with shell-like SNRs, suggesting that at least some MMSNRs have experienced similar evolution to shell-like SNRs. In addition to the results above, we have discovered an ``overionized'' ionization state in W49B, in addition to that previously found in IC 443. Thermal conduction can cause the hot interior plasma to become overionized by reducing the temperature and density gradients, leading to an interior density increase and temperature decrease. Therefore, we suggest that the ``center-filled'' X-ray morphology develops as the result of thermal conduction, and should arise in all SNRs. This is consistent with the results that MMSNRs are near collisional ionization equilibrium since the conduction timescale is roughly similar to the ionization timescale. Hence, we conclude that MMSNRs are those that have evolved over$\sim10^4$ yr. We call this phase as the ``conduction phase.''Comment: 34 pages, 20 figures, 9 tables, accepted for publication in The Astrophysical Journa