Bisphenol A Blood and Saliva Levels Prior to and after Dental Sealant Placement in Adults

Purpose: This study examined the effects of a widely used (Delton® Pit & Fissure Sealant – Light Cure Opaque, DENTSPLY Professional, York, PA) pit and fissure sealant material on bisphenol A (BPA) levels in blood and saliva, among both low and high–dose groups over time. Methods: A convenience sample of 30 adults from the Old Dominion University population were randomly and evenly divided into 2 independent variable groups: a low–dose group (1 occlusal sealant application) and high–dose group (4 occlusal sealant applications). A 2 group, time series design was used to examine the presence and concentration of BPA in serum and saliva after sealant placement. Differences comparing low–dose and high–dose groups were examined 1 hour prior (baseline), 1 hour post, 3 hours post and 24 hours after sealant placement, as measured by a direct–competitive BPA Enzyme Linked ImmunoSorbent Assay (ELISA). Hypothesized outcomes were evaluated by applying a parametric, 2 way ANOVA for repeated measures technique to data on the 30 participants ranging in age from 18 to 40 years, and were of mixed gender and ethnicity. Results: BPA was detected in the saliva of all participants prior to sealant placement and ranged from 0.07 to 6.00 ng/ml at baseline. Salivary BPA concentration levels peaked over a 3 hour period following sealant placement and returned to baseline levels within 24 hours. BPA was significantly elevated at all post–sealant placement time periods for both the low–dose (1 occlusal sealant application) and high–dose (4 occlusal sealant applications) groups with peak levels of 3.98 ng/ml and 9.08 ng/ml, respectively. The blood serum did not contain BPA at any point in this investigation. Conclusions: Exposure to BPA from sources other than dental resins contributes to salivary baseline concentration levels and indicates environmental exposure and use of products containing BPA. Use of specific molecular formulations of dental sealant material determines the release of BPA, therefore, dental sealant materials should be reviewed independently when questioning the release of BPA from dental sealants. In addition, dosage amounts of the dental sealant material used in this study do not influence the serum concentration levels of BPA. Further research is needed to examine the cumulative estrogenic effects of BPA from dental sealants

Concert recording 2016-11-13a

[Track 1]. Partita for English horn and organ. Andante sostenuto / Jan Koetsier -- [Track 2]. Niobe / Thea Musgrave -- [Track 3]. Wind quintet in G minor. Allegro / Paul Taffanel -- [Tracks 4-5]. Koncertstuck fur oboe / Julius Rietz

Legionella Eukaryotic-Like Type IV Substrates Interfere with Organelle Trafficking

Legionella pneumophila, the causative agent of Legionnaires' disease, evades phago-lysosome fusion in mammalian and protozoan hosts to create a suitable niche for intracellular replication. To modulate vesicle trafficking pathways, L. pneumophila translocates effector proteins into eukaryotic cells through a Type IVB macro-molecular transport system called the Icm-Dot system. In this study, we employed a fluorescence-based translocation assay to show that 33 previously identified Legionella eukaryotic-like genes (leg) encode substrates of the Icm-Dot secretion system. To assess which of these proteins may contribute to the disruption of vesicle trafficking, we expressed each gene in yeast and looked for phenotypes related to vacuolar protein sorting. We found that LegC3-GFP and LegC7/YlfA-GFP caused the mis-secretion of CPY-Invertase, a fusion protein normally restricted to the yeast vacuole. We also found that LegC7/YlfA-GFP and its paralog LegC2/YlfB-GFP formed large structures around the yeast vacuole while LegC3-GFP localized to the plasma membrane and a fragmented vacuole. In mammalian cells, LegC2/YlfB-GFP and LegC7/YlfA-GFP were found within large structures that co-localized with anti-KDEL antibodies but excluded the lysosomal marker LAMP-1, similar to what is observed in Legionella-containing vacuoles. LegC3-GFP, in contrast, was observed as smaller structures which had no obvious co-localization with KDEL or LAMP-1. Finally, LegC3-GFP caused the accumulation of many endosome-like structures containing undigested material when expressed in the protozoan host Dictyostelium discoideum. Our results demonstrate that multiple Leg proteins are Icm/Dot-dependent substrates and that LegC3, LegC7/YlfA, and LegC2/YlfB may contribute to the intracellular trafficking of L. pneumophila by interfering with highly conserved pathways that modulate vesicle maturation

NeuronBank: A Tool for Cataloging Neuronal Circuitry

The basic unit of any nervous system is the neuron. Therefore, understanding the operation of nervous systems ultimately requires an inventory of their constituent neurons and synaptic connectivity, which form neural circuits. The presence of uniquely identifiable neurons or classes of neurons in many invertebrates has facilitated the construction of cellular-level connectivity diagrams that can be generalized across individuals within a species. Homologous neurons can also be recognized across species. Here we describe NeuronBank.org, a web-based tool that we are developing for cataloging, searching, and analyzing neuronal circuitry within and across species. Information from a single species is represented in an individual branch of NeuronBank. Users can search within a branch or perform queries across branches to look for similarities in neuronal circuits across species. The branches allow for an extensible ontology so that additional characteristics can be added as knowledge grows. Each entry in NeuronBank generates a unique accession ID, allowing it to be easily cited. There is also an automatic link to a Wiki page allowing an encyclopedic explanation of the entry. All of the 44 previously published neurons plus one previously unpublished neuron from the mollusc, Tritonia diomedea, have been entered into a branch of NeuronBank as have 4 previously published neurons from the mollusc, Melibe leonina. The ability to organize information about neuronal circuits will make this information more accessible, ultimately aiding research on these important models

The Ursinus Weekly, March 3, 1978

Ursinus news in brief: Fields gets Ph.D.; Ursinus appoints P.R. director; Dining committee meets; P.E. speakers; Pollick attends pool finals • Energy conservation: The Choice is yours • New gym hours proposed • Sakell replaces Dempsey • Cat placed • Comment: Testimonials • Union answers questions • Letters to the editor • Ruby seeks editor • As you like it: Out of this world • The \u2778 Lantern • Cogger announces placement services • Puppeteer performs • College Bowl held • Crosswinds comes to Ursinus • Grapplers troubled • Four meet streak • Victory for women\u27s b-ballhttps://digitalcommons.ursinus.edu/weekly/1082/thumbnail.jp

Concert recording 2013-11-15

[Track 01]. Amarilli, mia bella / Guilio Caccini ; [Track 02]. Dein blaues auge / Johannes Brahms -- [Track 03]. Come raggio di sol / Antonio Caldara -- [Track 04]. Come ready and see me / Richard Hundley -- [Track 05]. La promessa / Giacchino Rossini -- [Track 06]. Se tu m\u27ami / Giovanni Pergolesi -- [Track 07]. Sonntag / Johannes Brahms -- [Track 08]. Le colibri / Ernest Chausson -- [Track 09]. Paulus. Jerusalem! / Felix Mendelssohn -- [Track 10]. Gia il sole dal Gange / Alessandro Scarlatti -- [Track 11]. Go, lovely Rose / Roger Quilter -- [Track 12]. Si mes vers avaient des ailes / Raynaldo Hahn -- [Track 13]. I\u27m a person too / Leonard Bernstein -- [Track 14]. Les chemins de l\u27amour / Francis Poulenc -- [Track 15]. La Boheme. Donde lieta usci al tuo... / Giacomo Puccini -- [Track 16]. Plum pudding / Leonard Bernstein -- [Track 17]. Queues de boeuf / Leonard Bernstein -- [Track 18]. Tavouk gueunksis / Leonard Bernstein -- [Track 19]. Civet a toute vitesse / Leonard Bernstein -- [Track 20]. When I have sung my songs to you / Ernest Charles -- [Track 21]. Linda di chamournix. Ah! Tardai troppo...o luce di quest\u27anima / Gaetano Donizetti

Concert recording 2018-04-20a

[Track 1]. Caro mio ben / Giuseppe Giordani -- [Track 2]. Alma del core / Antonio Caldara -- [Track 3]. A little bit in love from Wonderful town / Bernstein -- [Track 4]. Im Abendrot / Franz Schubert -- [Track 5]. Something\u27s coming from West side story / Bernstein -- [Track 6]. Che far senza Euridice from Orfeo e Euridice / Christoph Willibald Gluck -- [Track 7]. What a movie from Trouble in Tahiti / Bernstein -- [Track 8]. Simple song from Mass / Bernstein -- [Track 9]. Round midnight / Thelonius Monk -- [Track 10]. My house from Peter Pan / Bernstein -- [Track 11]. Heaven help my heart from Chess / Andersson & Ulvaeus -- [Track 12]. L\u27heure exquise / Reynaldo Hahn -- [Track 13]. Captain Hook\u27s soliloquy from Peter Pan / Bernstein -- [Track 14]. Two love songs. Extinguish my eyes [Track 15]. When my soul touches yours / Bernstein -- [Track 16]. Go on from Mass / Bernstein -- [Track 17]. Wanderers Nachtlied / Schubert -- [Track 18]. Peter, Peter from Peter Pan / Bernstein -- [Track 19]. L\u27escalve / Édouard Lalo -- [Track 20]. Allerseelen / Richard Strauss -- [Track 21]. Nothing more than this from Candide / Bernstein -- [Track 22]. O del mio dolce ardor / Gluck -- [Track 23]. Governer\u27s serenade from Candide / Bernstein -- [Track 24]. Sogno d\u27or / Giacomo Puccini -- [Track 25]. I understand from On the town / Bernstein -- [Track 26]. Dream with me from Peter Pan / Bernstein -- [Track 27]. Il est doux, il est bon from Herodiade / Jules Massenet -- [Track 28]. Easter from Five mystical songs / Ralph Vaughan Williams -- [Track 29]. One hand, one heart from West side story / Bernstein -- [Track 30]. Porgi amor from Les Nozze di Figaro / Wolfgang Amadeus Mozart -- [Track 31]. Glitter and be gay from Candide / Bernstein