Additional file 1: Figure S1. of Sex differences in porcine left ventricular myocardial remodeling due to right ventricular pacing

Indices of LV function at different time points for sham-operated pigs and RV-paced pigs. A and B show changes over time in LVPWd (LVPWd—end-diastolic thickness of left ventricle posterior wall); C and D show LVPWs—end-systolic thickness of left ventricle posterior wall, E and F show SV (stroke volume) in right ventricular (RV) paced and sham-operated pigs; G and H show LA/Ao (left atrial/aorta ratio) separately in male and female animals (black squares—RV paced female pigs, black circles—RV paced male pigs, gray squares—female controls, gray circles—male controls). Values are presented as means ± SEM. Data (separately for control and TIC animals) were tested using one-way ANOVA. Figure S2. Relationship between BNP mRNA expression and echocardiographic parameters. Elevated BNP mRNA was significantly correlated with increased LVEDV in males (A) as well as with a decrease in LVEF in males (B) and females (C). Regression lines have been fit to all data points. Figure S3. Relationship between GATA4 mRNA expression and TGFβ1 and BNP expression level as well as LVEF. Elevated GATA4 mRNA was significantly correlated with increased BNP mRNA level in males (A) as well as in females (B). Only in males elevated GATA4 mRNA was also significantly correlated with TGFβ1 BNP mRNA level (C) and with a decrease in LVEF (D). Regression lines have been fit to all data points. Figure S4. Relationship between NGAL mRNA expression and LVEDV. Elevated NGAL mRNA was significantly correlated with increased LVEDV in males. Regression lines have been fit to all data points. Figure S5. Relationship between collagen mRNA level and TGFβ1 expression. Elevated Col1A1 (A), Col1A2 (B), and Col3A1 (C) mRNAs were significantly correlated with an increase in TGFβ1 mRNA level in males. In females increased expression of Col1A1 (D) and Col3A1 (E) was related to TGFβ1 mRNA level. Regression lines have been fit to all data points. Figure S6. Relationship between ECM turnover markers and echocardiographic parameters. (A) Reduced total gelatinolytic activity was significantly correlated with an increase in BNP mRNA level in males. (B) Decreasing water soluble collagen was related to an decrease in NGAL mRNA. (C) In male subjects from the mild HF group, the water soluble collagen correlated with the relative increase in LVEDV. Regression lines have been fit to all data points. Figure S7. Relationship between cardiomyocyte diameter and echocardiographic parameters. In females cardiomyocyte cross-sectional diameter correlated with LVEDV. Regression lines have been fit to all data points. Table S1. Initial values of echocardiography parameters in sham-operated male pigs (controls) and right ventricle paced pigs with induced heart failure. Table S2. Echocardiography parameters reflecting the structure and functioning of left ventricle in sham-operated male pigs (controls) and right ventricle paced pigs with induced heart failure. Table S3. Sex-related differences in selected echocardiography parameters assessed in left ventricular myocardium in male pigs with and without tachycardia-induced cardiomyopathy (the results of the two-way ANOVA). Table S4. Relationships between selected echocardiographic parameters and HF insufficiency in male and female pigs with and without tachycardia-induced cardiomyopathy (the results of Spearman’s rank correlatory coefficients)