1,013 research outputs found
RR Lyrae Variables in M33. I. Evidence For a Field Halo Population
We present observations of RR Lyrae variables in the Local Group late-type
spiral galaxy M33. Using the Advanced Camera for Surveys on the Hubble Space
Telescope, we have identified 64 ab-type RR Lyraes in M33. We have estimated
reddenings for these stars based on their minimum light V-I colors and
metallicities based on their periods. From the distributions of these
properties, we conclude that the RR Lyraes belong to two populations - one
associated with the halo of M33 and the other with its disk. Given that RR
Lyraes are produced by populations older than ~10 Gyr, this suggests that not
only does the field halo of M33 contain an old component, but so does its disk.
This is one of the best pieces of evidence for the existence of a halo field
component in M33. Using a relation between RR Lyrae absolute magnitude and
metallicity (Mv(RR) = 0.23[Fe/H] + 0.93), we estimate a mean distance modulus
of = 24.67 +/- 0.08 for M33. This places M33 approximately 70 kpc
beyond M31 in line-of-sight distance.Comment: 20 pages, 17 figures, accepted for publication in The Astronomical
Journa
α-Synuclein Concentration Increases Over Time in Plasma Supernatant of Single Donor Platelets
OBJECTIVES: In platelets, α-synuclein is important in calcium-dependent granule release. Notably, cells release α-synuclein in setting of cell damage or death. Therefore, we investigated α-synuclein levels in plasma of single donor platelet (SDP) units during storage. METHODS: Aliquots were obtained from same SDP units for 7 days from day of donation. Additionally, randomly sampled SDP units at same storage time points were also assayed by enzyme-linked immunosorbent assay. RESULTS: α-Synuclein in SDP plasma increased continuously over time at each assayed time point. Significant increases were measured on day 3 (11.7 ± 9.6 ng/mL, P = 0.025), day 5 (15.3 ± 5.9 ng/mL, P = 0.002), and highest on day 7 (23.7 ± 5.6 ng/mL, P \u3c 0.0001) compared to day 0 (1.1 ± 0.8 ng/mL). Similar significant results were obtained in randomly sampled SDP units at same corresponding time points. Flow cytometry showed that platelets had strong expression of α-synuclein and lacked expression of other synucleins. CONCLUSIONS: Increases of α-synuclein during SDP storage is a steady and continuous process that increases with time. Our findings indicate that α-synuclein may represent a biomarker of platelet biological state during storage. Further research will be needed to determine how α-synuclein increases correlate with platelets\u27 function
Use of a Whole-Cell ELISA to Detect Additional Antibodies in Setting of Suspected Heparin-Induced Thrombocytopenia
OBJECTIVES: Type II heparin-induced thrombocytopenia (HIT) is mediated by formation of antibodies to platelet factor 4 (PF4)-heparin complexes. We evaluated anti-PF4-heparin-negative samples for the presence of additional anti-platelet and anti-red blood cell (RBC) antibodies using whole-cell platelet/ RBC ELISAs we developed. METHODS: Seventy-three samples tested for anti-PF4-heparin by ELISA were included: 62 tested negative, 9 tested positive, and 2 had equivocal results. Plasma specimens from healthy donors were used as controls. RESULTS: 100% (9/9) anti-PF4-positive samples had anti-platelet antibodies detected by whole-cell platelet ELISA. 42.2% (27/64) anti-PF4-heparin-negative samples were negative for anti-platelet and anti-RBC antibodies. 32.8% (21/64) negative samples showed reactivity to both platelets and RBC; 12.5% (8/64) negative samples were each reactive with either platelet or RBC ELISA, respectively. Additionally, two samples that tested equivocal by anti-PF4-heparin ELISA had antibodies to both platelets and RBC by whole-cell ELISA. CONCLUSIONS: Our study suggests that patients with thrombocytopenia testing negative for anti-PF4-heparin may still harbor antibodies to platelets. However, additional research is needed to determine the significance of these antibodies. Nevertheless, these findings may encourage clinicians to further investigate patients with possible immune-mediated etiologies of thrombocytopenia and anemia
Infectivity of an Infectious Clone of Banana Streak CA Virus in A-Genome Bananas (Musa acuminata ssp.)
We have characterized the complete genome sequence of an Australian isolate of banana streak CA virus (BSCAV). A greater-than-full-length, cloned copy of the virus genome was assembled and agroinoculated into five tissue-cultured plants of nine different Musa acuminata banana accessions. BSCAV was highly infectious in all nine accessions. All five inoculated plants from eight accessions developed symptoms by 28 weeks post-inoculation, while all five plants of M. acuminata AA subsp. zebrina remained symptomless. Symptoms were mild in six accessions but were severe in Khae Phrae (M. acuminata subsp. siamea) and the East African Highland banana accession Igisahira Gisanzwe. This is the first full-length BSCAV genome sequence reported from Australia and the first report of the infectivity of an infectious clone of banana streak virus
Structural diversity in the type IV pili of multidrug-resistant Acinetobacter
Acinetobacter baumannii is a Gram-negative coccobacillus found primarily in hospital settings that has recently emerged as a source of hospital-acquired infections. A. baumannii expresses a variety of virulence factors, including type IV pili, bacterial extracellular appendages often essential for attachment to host cells. Here, we report the high resolution structures of the major pilin subunit, PilA, from three Acinetobacter strains, demonstrating thatA. baumannii subsets produce morphologically distinct type IV pilin glycoproteins. We examine the consequences of this heterogeneity for protein folding and assembly as well as host-cell adhesion by Acinetobacter. Comparisons of genomic and structural data with pilin proteins from other species of soil gammaproteobacteria suggest that these structural differences stem from evolutionary pressure that has resulted in three distinct classes of type IVa pilins, each found in multiple species
Kinematics of Metal-Poor Stars in the Galaxy. II. Proper Motions for a Large Non-Kinematically Selected Sample
We present a revised catalog of 2106 Galactic stars, selected without
kinematic bias, and with available radial velocities, distance estimates, and
metal abundances in the range 0.0 <= [Fe/H] <= -4.0. This update of the Beers
and Sommer-Larsen (1995) catalog includes newly-derived homogeneous photometric
distance estimates, revised radial velocities for a number of stars with
recently obtained high-resolution spectra, and refined metallicities for stars
originally identified in the HK objective-prism survey (which account for
nearly half of the catalog) based on a recent re-calibration. A subset of 1258
stars in this catalog have available proper motions, based on measurements
obtained with the Hipparcos astrometry satellite, or taken from the updated
Astrographic Catalogue (AC 2000; second epoch positions from either the Hubble
Space Telescope Guide Star Catalog or the Tycho Catalogue), the Yale/San Juan
Southern Proper Motion (SPM) Catalog 2.0, and the Lick Northern Proper Motion
(NPM1) Catalog. Our present catalog includes 388 RR Lyrae variables (182 of
which are newly added), 38 variables of other types, and 1680 non-variables,
with distances in the range 0.1 to 40 kpc.Comment: 31 pages, including 8 figures, to appear in AJ (June 2000), full
paper with all figures embedded available at
http://pluto.mtk.nao.ac.jp/people/chiba/preprint/halo4
The Hubble Space Telescope Extragalactic Distance Scale Key Project. X. The Cepheid Distance to NGC 7331
The distance to NGC 7331 has been derived from Cepheid variables observed
with HST/WFPC2, as part of the Extragalactic Distance Scale Key Project.
Multi-epoch exposures in F555W (V) and F814W (I), with photometry derived
independently from DoPHOT and DAOPHOT/ALLFRAME programs, were used to detect a
total of 13 reliable Cepheids, with periods between 11 and 42 days. The
relative distance moduli between NGC 7331 and the LMC, imply an extinction to
NGC 7331 of A_V = 0.47+-0.15 mag, and an extinction-corrected distance modulus
to NGC 7331 of 30.89+-0.14(random) mag, equivalent to a distance of 15.1 Mpc.
There are additional systematic uncertainties in the distance modulus of +-0.12
mag due to the calibration of the Cepheid Period-Luminosity relation, and a
systematic offset of +0.05+-0.04 mag if we applied the metallicity correction
inferred from the M101 results of Kennicutt et al 1998.Comment: To be published in The Astrophysical Journal, 1998 July 1, v501 note:
Figs 1 and 2 (JPEG files) and Fig 7 (multipage .eps file) need to be
viewed/printed separatel
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