Validation of expression levels of miRNA biomarkers derived from the platelet fraction.

<p>miRNA expression levels of 7 biomarkers (n = 4, *P values<0.001) were validated by qPCR. The p values are calculated based on a Student's t-test of the replicate 2∧ (−ΔCt) values for each miRNA in the control group (normal individuals) and test groups (patients).</p

Sub-clusters of platelet-derived miRNA biomarkers.

<p>Unsupervised hierarchical clustering of miRNA biomarkers based on the Pearson correlation coefficients among the miRNA expression profiles from 20 patients and 20 controls. The coefficient values are shown in the bar scale. Each of the 4 main clusters with significant correlation values are indicated in boxes.</p

Workflow for biomarker derivation and computation of predictive estimates.

<p>(A) Differentially expressed miRNAs were first derived by SAM and those exhibiting a FDR of <1% and frequency of occurrence >90% in 100 iterations, were selected as biomarkers. (B) The decision rules were obtained by SVM classification and the predictive estimates of the selected biomarkers determined by 10-fold cross-validation.</p

Demographics of individuals recruited for this study.

<p>Gender is designated as either M (Male) or F (Female). Disorders are designated as GI (Gastro-intestinal) or CD (Crohn's Disease).</p