Hydrodynamic stability of a sheared liquid film

We study the hydrodynamic stability of a thin layer of liquid that is sheared by a gas. First, the interface conditions for the free surface approximation of the problem are discussed. We then study the stability of the flow to disturbances with phase speeds smaller than the maximum velocity in the liquid film, i.e. the internal mode, extending previous results and resolving some apparent contradictions. The dynamic effect of the gas is studied by dropping the free surface approximation and solving the Orr-Sommerfeld equation for the gas together with that for the liquid. The effect on the stability of the liquid film is very large, which is explained by the fact that the imaginary part of the wave speed (which determines the stability of the film) is very small. Consequently the free surface approximation is, in general, not correct. We then study the dependence of the critical Reynolds number on the Weber number, on the curvature of the liquid velocity profile and on the properties of the gas. With the gas included, a second mode of instability is found which has a phase velocity that is, in general, larger than the maximum liquid velocity and corresponds to capillary-gravity waves. We compare results with experiments from the literature; good agreement is found. Finally, a suggestion on the relevance of this study to the generation of ‘roll waves’, which are important from a practical point of view, is given

A DNA virus-encoded immune antagonist fully masks the potent antiviral activity of RNAi in Drosophila

International audienceCoevolution of viruses and their hosts may lead to viral strategies to avoid, evade, or suppress antiviral immunity. An example is antiviral RNA interference (RNAi) in insects: the host RNAi machinery processes viral double-stranded RNA into small interfering RNAs (siRNAs) to suppress viral replication, whereas insect viruses encode suppressors of RNAi, many of which inhibit viral small interfering RNA (vsiRNA) production. Yet, many studies have analyzed viral RNAi suppressors in heterologous systems, due to the lack of experimental systems to manipulate the viral genome of interest, raising questions about in vivo functions of RNAi suppressors. To address this caveat, we generated an RNAi suppressor-defective mutant of invertebrate iridescent virus 6 (IIV6), a large DNA virus in which we previously identified the 340R protein as a suppressor of RNAi. Loss of 340R did not affect vsiRNA production, indicating that 340R binds siRNA duplexes to prevent RNA-induced silencing complex assembly. Indeed, vsiRNAs were not efficiently loaded into Argonaute 2 during wild-type IIV6 infection. Moreover, IIV6 induced a limited set of mature microRNAs in a 340R-dependent manner, most notably miR-305-3p, which we attribute to stabilization of the miR-305-5p:3p duplex by 340R. The IIV6 340R deletion mutant did not have a replication defect in cells, but was strongly attenuated in adult Drosophila. This in vivo replication defect was completely rescued in RNAi mutant flies, indicating that 340R is a bona fide RNAi suppressor, the absence of which uncovers a potent antiviral immune response that suppresses virus accumulation ∼100-fold. Together, our work indicates that viral RNAi suppressors may completely mask antiviral immunity